公开(公告)号：US20160060588A1

公开(公告)日：2016-03-03

申请号：US14771358

申请日：2015-02-20

Applicant: Kyoto University ,  Nipro Corporation

Inventor： Norio Nakatsuji ,  Yoshihiro Yoshikawa ,  Masakatsu Takeuchi ,  Daiki Tateyama

IPC: C12M1/00 ,  C12M1/12 ,  C12M1/34

CPC classification number: C12M23/58 ,  C12M23/14 ,  C12M25/02 ,  C12M29/00 ,  C12M29/04 ,  C12M41/00

Abstract: A culture system has a culture bag for suspending and culturing pluripotent stem cells in a culture medium; a waste liquid container for storing used culture medium that is connected to the culture bag; a fresh medium container for storing a fresh culture medium that is connected to the culture bag; three-way stopcocks for switching flow channels from the culture bag to the waste liquid container or the fresh culture medium container, etc.; a trap portion for trapping the pluripotent stem cells in the culture medium in the flow toward the waste liquid container side; and a filter portion for subculturing that is formed in fourth and fifth flow channels in parallel to a first flow channel. The first flow channel connects the trap part to the culture bag, and has a mesh by which a cell mass of the pluripotent stem cells can be divided. A pump is used to pump the liquid in the individual flow channels.

Abstract translation: 培养系统具有用于在培养基中悬浮培养多能干细胞的培养袋; 用于储存连接到培养袋的使用的培养基的废液容器; 用于储存连接到培养袋的新鲜培养基的新鲜培养基容器; 用于从培养袋切换到废液容器或新鲜培养基容器等的流动通道的三通旋塞阀; 用于将培养液中的多能干细胞捕获到向废液容器侧流动的捕获部分; 以及用于传代培养的过滤器部分，其形成在与第一流动通道平行的第四和第五流动通道中。 第一流动通道将捕获部分连接到培养袋，并且具有可以分开多能干细胞的细胞块的网。 泵用于在各个流动通道中泵送液体。