公开(公告)号：US20190112626A1

公开(公告)日：2019-04-18

申请号：US16090640

申请日：2017-03-30

Applicant: President and Fellows of Harvard College

Inventor： Henry Hung-yi Lee ,  George M. Church ,  Reza Kalhor

IPC: C12P19/34

Abstract: The disclosure provides methods for making a polynucleotide wherein the addition of nucleotides can be physically, chemically and/or enzymatically controlled. The methods include combining a selected nucleotide, cations, an error prone or template independent DNA polymerase at a reaction site including an initiator sequence attached thereto and having a 3′ terminal nucleotide, wherein the reaction reagents can be modulated and under conditions that allow covalent addition of one or more of a selected nucleotide to the 3′ terminal nucleotide such that the selected nucleotide becomes a 3′ terminal nucleotide, and repeating the addition step until the polynucleotide is formed.