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公开(公告)号：US20230272380A1
公开(公告)日：2023-08-31
申请号：US18313482
申请日：2023-05-08
Applicant: President and Fellows of Harvard College
Inventor： Reza Kalhor , Prashant G. Mali , George M. Church
IPC: C12N15/11 , C12N15/10 , C12Q1/6853 , C12N9/22 , C12N15/113
CPC classification number: C12N15/11 , C12N15/1096 , C12Q1/6853 , C12N15/10 , C12N9/22 , C12N15/113 , C12N2310/20 , C12N2310/3519 , C12N2310/531
Abstract: A functional engineered guide RNA sequence is provided including a spacer sequence and a scaffold sequence, wherein the scaffold sequence includes a primer binding site for reverse transcription.
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公开(公告)号：US10851369B2
公开(公告)日：2020-12-01
申请号：US16310643
申请日：2017-06-21
Applicant: President and Fellows of Harvard College
Inventor： Reza Kalhor , Javier Fernandez Juarez , Henry Hung-yi Lee , George M. Church
Abstract: A method of processing a collection of nucleic acid sequences is provided including connecting an adaptor to one or more or each nucleic acid sequence in the collection to create a processed nucleic acid template library, wherein the adaptor includes a first DNA sequence encoding a PAM sequence and at least a tracr mate.
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公开(公告)号：US10745814B2
公开(公告)日：2020-08-18
申请号：US16099487
申请日：2017-05-09
Applicant: President and Fellows of Harvard College
Inventor： Reza Kalhor , Henry Hung-yi Lee , George M. Church
IPC: C25B3/10 , C12Q1/6844 , C12P19/34 , C25B3/02 , C25B3/04 , C12Q1/6806
Abstract: The present disclosure provides methods of activating an enzyme, such as error prone or template independent polymerase, using electricity to alter pH of a reaction zone and reaction site from an inactivating pH at which the enzyme is inactive to an activating pH at which the enzyme is active to add a nucleotide to an initiator or growing polymer chain. The activating pH can then be changed back to an inactivating pH and the process repeated as many times as desired to produce a target nucleic acid sequence.
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公开(公告)号：US20190264202A1
公开(公告)日：2019-08-29
申请号：US16346569
申请日：2017-11-01
Applicant: President and Fellows of Harvard College
Inventor： George M. Church , Reza Kalhor , Prashant G. Mali
IPC: C12N15/11 , C12Q1/6853 , C12N15/10
Abstract: A functional engineered guide RNA sequence is provided including a spacer sequence and a scaffold sequence, wherein the scaffold sequence includes a primer binding site for reverse transcription.
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公开(公告)号：US20180057867A1
公开(公告)日：2018-03-01
申请号：US15800291
申请日：2017-11-01
Applicant: President and Fellows of Harvard College
Inventor： Chao-ting Wu , Reza Kalhor
IPC: C12Q1/68
CPC classification number: C12Q1/6832 , C12Q2537/162 , C12Q2543/10
Abstract: The present invention relates to methods of hybridizing nucleic acid probes to genomic DNA.
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Patent Agency Ranking
公开(公告)号：US20190177720A1
公开(公告)日：2019-06-13
申请号：US16310643
申请日：2017-06-21
Applicant: President and Fellows of Harvard College
Inventor： Reza Kalhor , Javier Fernandez Juarez , Henry Hung-yi Lee , George M. Church
Abstract: A method of processing a collection of nucleic acid sequences is provided including connecting an adaptor to one or more or each nucleic acid sequence in the collection to create a processed nucleic acid template library, wherein the adaptor includes a first DNA sequence encoding a PAM sequence and at least a tracr mate.
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公开(公告)号：US11732258B2
公开(公告)日：2023-08-22
申请号：US16346569
申请日：2017-11-01
Applicant: President and Fellows of Harvard College
Inventor： Reza Kalhor , Prashant G. Mali , George M. Church
IPC: C12N15/00 , C12N15/11 , C12N15/10 , C12Q1/6853 , C12N9/22 , C12N15/113
CPC classification number: C12N15/11 , C12N9/22 , C12N15/10 , C12N15/1096 , C12N15/113 , C12Q1/6853 , C12N2310/20 , C12N2310/3519 , C12N2310/531 , C12Q1/6853 , C12Q2521/107 , C12Q2525/155 , C12Q2525/197 , C12Q2525/301
Abstract: A functional engineered guide RNA sequence is provided including a spacer sequence and a scaffold sequence, wherein the scaffold sequence includes a primer binding site for reverse transcription.
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公开(公告)号：US20210108243A1
公开(公告)日：2021-04-15
申请号：US16951254
申请日：2020-11-18
Applicant: President and Fellows of Harvard College
Inventor： Henry Hung-yi Lee , George M. Church , Reza Kalhor
IPC: C12P19/34 , C12Q1/6844
Abstract: The disclosure provides methods for making a polynucleotide wherein the addition of nucleotides can be physically, chemically and/or enzymatically controlled. The methods include combining a selected nucleotide, cations, an error prone or template independent DNA polymerase at a reaction site including an initiator sequence attached thereto and having a 3′ terminal nucleotide, wherein the reaction reagents can be modulated and under conditions that allow covalent addition of one or more of a selected nucleotide to the 3′ terminal nucleotide such that the selected nucleotide becomes a 3′ terminal nucleotide, and repeating the addition step until the polynucleotide is formed.
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公开(公告)号：US20190161743A1
公开(公告)日：2019-05-30
申请号：US16099473
申请日：2017-05-09
Applicant: President and Fellows of Harvard College
Inventor： George M. Church , Reza Kalhor , Prashant G. Mali
Abstract: CRISPR/Cas9 methods are provided where a guide RNA is engineered to self-target and inactivate a nucleic acid encoding the guide RNA itself.
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公开(公告)号：US20190145013A1
公开(公告)日：2019-05-16
申请号：US16099487
申请日：2017-05-09
Applicant: President and Fellows of Harvard College
Inventor： Reza Kalhor , Henry Hung-yi Lee , George M. Church
Abstract: The present disclosure provides methods of activating an enzyme, such as error prone or template independent polymerase, using electricity to alter pH of a reaction zone and reaction site from an inactivating pH at which the enzyme is inactive to an activating pH at which the enzyme is active to add a nucleotide to an initiator or growing polymer chain. The activating pH can then be changed back to an inactivating pH and the process repeated as many times as desired to produce a target nucleic acid sequence.
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