公开(公告)号：US20230183716A1

公开(公告)日：2023-06-15

申请号：US17870029

申请日：2022-07-21

Applicant: RAMOT AT TEL-AVIV UNIVERSITY LTD.

Inventor： Tamir TULLER ,  Michael PEERI

IPC: C12N15/68 ,  C12N15/69 ,  C12N15/10

CPC classification number: C12N15/68 ,  C12N15/69 ,  C12N15/102

Abstract: Nucleic acid molecule comprising a coding sequence and a region of increased folding energy upstream of a stop codon are provided. Expression vectors and cells comprising the nucleic acid molecule are also provided. Methods for optimizing a coding sequence comprising increasing folding energy in a region upstream of the stop codon are also provided.

公开(公告)号：US20220396801A1

公开(公告)日：2022-12-15

申请号：US17870607

申请日：2022-07-21

Applicant: B. G. NEGEV TECHNOLOGIES AND APPLICATIONS LTD., AT BEN-GURION UNIVERSITY ,  RAMOT AT TEL-AVIV UNIVERSITY LTD

Inventor： Tamir TULLER ,  Michael PEERI ,  Yonatan CHEMLA ,  Lital ALFONTA

IPC: C12N15/67

Abstract: Nucleic acid molecule and vectors comprising regions of high or low folding energy are provided. Methods of producing coding sequences optimized for protein expression comprising introducing a mutation that increases or decreased folding energy are also provided.

公开(公告)号：US20220162595A1

公开(公告)日：2022-05-26

申请号：US17486936

申请日：2021-09-28

Applicant: RAMOT AT TEL-AVIV UNIVERSITY LTD.

Inventor： Tamir TULLER ,  Shir BAHIRI ,  Boaz APT

IPC: C12N15/10 ,  C12N15/67

Abstract: Nucleic acid molecules comprising a mutation that mutation modulates the interaction strength of the nucleic acid molecule to a 16S ribosomal RNA are provided. Methods of improving the translation process of a nucleic acid molecule and producing a nucleic acid molecule optimized for translation, as well as cells comprising the nucleic acid molecules are also provided.