公开(公告)号：US20110177602A1

公开(公告)日：2011-07-21

申请号：US13000385

申请日：2009-06-24

申请人： Dietmar Appelhans ,  Mathias Lakatos ,  Wolfgang Pompe

发明人： Dietmar Appelhans ,  Mathias Lakatos ,  Wolfgang Pompe

IPC分类号： G01N31/22 ,  B82Y15/00

CPC分类号： G01N33/587 ,  B82Y5/00 ,  G01N33/54346 ,  G01N33/54373 ,  G01N2333/395 ,  Y10T436/107497

摘要： A composite structure, for marking biomolecules in a biological, biochemical or medicinal system, comprises: at least one nano particle and at least one dendritic macromolecule, which has an inner region with branched, especially perfectly branched to highly branched, structures and a periphery, which comprises surface groups of the dendritic macromolecule, wherein a plurality, especially more than 50%, of the surface groups have in the periphery of the dendritic macromolecule, in each case, at least one functional group of first type, wherein the functional group of first type comprises at least one monosaccharide-, oligosaccharide- and/or polysaccharide unit, and wherein the dendritic macromolecule stabilizes the nano particle.

摘要翻译： 用于在生物，生物化学或药物系统中标记生物分子的复合结构包括：至少一个纳米颗粒和至少一个树突状大分子，其具有带有支链，特别是完全分支到高度支化的结构和外围的内部区域， 其包括树枝状高分子的表面基团，其中多个，特别是大于50％的表面基团在树突状高分子的周围具有至少一种第一类型的官能团，其中所述官能团为 第一类型包含至少一种单糖，寡糖和/或多糖单元，并且其中所述树突状高分子使纳米颗粒稳定。

公开(公告)号：US20100285505A1

公开(公告)日：2010-11-11

申请号：US12666299

申请日：2008-06-27

申请人： Kai Ostermann ,  Wolfgang Pompe ,  Dagmar Wersing ,  Mathias Lakatos ,  Michael Mertig ,  Gerhard Rödel ,  Simone Thierfelder

发明人： Kai Ostermann ,  Wolfgang Pompe ,  Dagmar Wersing ,  Mathias Lakatos ,  Michael Mertig ,  Gerhard Rödel ,  Simone Thierfelder

IPC分类号： G01N33/53 ,  C12M1/34

CPC分类号： G01N33/587 ,  G01N33/54373 ,  G01N2333/395

摘要： The invention relates to devices and methods for detecting a substance by cell surface polarizations and the detection thereof by means of PPR or particle-mediated fluorescence. The device for detecting a substance by cell surface polarization according to the invention has cells, wherein a gene the expression of which leads to the polarized presentation of a protein on the surface of the cell is placed under the control of a promotor which can be regulated by the substance to be detected, nanoparticles which are functionalized with a molecule which can bind specifically to the surface-exposed protein, and at least one optical measurement device, such that an accumulation of the nanoparticles on the surface of the cells can be detected by particle plasmon resonance or particle-mediated fluorescence.

摘要翻译： 本发明涉及用于通过细胞表面极化检测物质的装置和方法，并且其通过PPR或颗粒介导的荧光进行检测。 根据本发明的用于通过细胞表面极化检测物质的装置具有细胞，其中表达导致细胞表面上的蛋白质的极化表达的基因置于可调节的启动子的控制下 通过待检测的物质，用能够与表面暴露蛋白特异性结合的分子官能化的纳米颗粒和至少一种光学测量装置，使得纳米颗粒在细胞表面上的积聚可以通过 颗粒等离子体共振或粒子介导的荧光。

公开(公告)号：US20100221817A1

公开(公告)日：2010-09-02

申请号：US12597296

申请日：2008-04-25

申请人： Kai Ostermann ,  Wolfgang Pompe ,  Horst Böttcher ,  Gerhard Rödel ,  Annett Gross

发明人： Kai Ostermann ,  Wolfgang Pompe ,  Horst Böttcher ,  Gerhard Rödel ,  Annett Gross

IPC分类号： C12M1/34

CPC分类号： C12Q1/02

摘要： The invention relates to whole-cell sensors for monitoring bioavailable nitrogen, phosphorus and sulphur, individually or in at least one combination in a medium, and to the use thereof. The whole-cell sensors consist of genetically modified yeast cells which are immobilised in a xerogel matrix and contain at least one marker gene controlled by a promoter of a gene, the transcription of said gene being significantly increased or reduced in the absence of nitrogen, phosphorus or sulphur, and the yeast cells are at least coupled to a signal detector.

摘要翻译： 本发明涉及用于单独监测生物可利用的氮，磷和硫的全细胞传感器，或在介质中的至少一种组合及其用途。 全细胞传感器由遗传修饰的酵母细胞组成，其固定在干凝胶基质中并含有至少一个由基因启动子控制的标记基因，所述基因的转录在不存在氮，磷的情况下显着增加或减少 或硫，并且酵母细胞至少耦合到信号检测器。

公开(公告)号：US06384196B1

公开(公告)日：2002-05-07

申请号：US09275397

申请日：1999-03-24

申请人： Karl Weis ,  Wolfgang Pompe ,  Jens Bradt

发明人： Karl Weis ,  Wolfgang Pompe ,  Jens Bradt

IPC分类号： A61K3817

CPC分类号： D01F4/00 ,  A61L27/425 ,  C08L89/06 ,  D01F1/10

摘要： The invention relates to a process for the preparation of mineralized collagen fibrils, where the fibril formation and mineralization take place in one process step, and to the use thereof as bone substitute material. The collagen is recombinant collagen.

摘要翻译： 本发明涉及一种用于制备矿化胶原纤维的方法，其中原纤维形成和矿化在一个工艺步骤中发生，并且其作为骨替代材料的用途。 胶原蛋白是重组胶原蛋白。