公开(公告)号：US06268127B1

公开(公告)日：2001-07-31

申请号：US09493352

申请日：2000-01-28

申请人： Lynn Bergmeyer ,  Kerry Lee Angie

发明人： Lynn Bergmeyer ,  Kerry Lee Angie

IPC分类号： C12Q168

CPC分类号： C12Q1/6806 ,  C12N15/1003 ,  C12Q2527/125 ,  C12Q2527/101

摘要： Described herein are methods for extracting DNA from serum or plasma, comprising contacting serum or plasma with alkali to yield alkalinized serum or plasma, heating the alkalinized serum or plasma to a temperature ranging from about 100 to about 110° C. for a time ranging from about 5 to about 20 minutes, centrifuging the heated alkalinized serum or plasma to yield a DNA-containing supernatant, allowing the heated alkalinized serum or plasma to cool to room temperature, or about 25° C., and recovering the DNA-containing supernatant. Also disclosed are methods for detecting a DNA-containing microorganism in serum or plasma.

摘要翻译： 本文描述的是从血清或血浆中提取DNA的方法，包括使血清或血浆与碱接触以产生碱化的血清或血浆，将碱化的血清或血浆加热至约100至约110℃的温度，时间范围为 约5至约20分钟，离心加热的碱化血清或血浆以产生含DNA的上清液，使加热的碱化血清或血浆冷却至室温或约25℃，并回收含DNA的上清液。 还公开了用于检测血清或血浆中含有DNA的微生物的方法。