公开(公告)号：US10907150B2

公开(公告)日：2021-02-02

申请号：US16008376

申请日：2018-06-14

Applicant: WISCONSIN ALUMNI RESEARCH FOUNDATION

Inventor： Jared Matthew Carlson-Stevermer ,  Krishanu Saha ,  Amr Ashraf Abdeen ,  Lucille Katherine Kohlenberg

IPC: C12N15/11 ,  C12N15/10 ,  C12N9/22 ,  C12N15/90

Abstract: Described herein are modified guide RNAs such as a single guide RNA including, from 5′ to 3′, a single-stranded protospacer sequence, a first complementary strand of a binding region for the Cas9 polypeptide, an aptamer that binds a biotin-binding molecule, and a second complementary strand of the binding region for the Cas9 polypeptide. Also described is an RNP complex including the modified guide RNA and a Cas9 polypeptide or active fragment thereof. Also included are methods of modifying target genes in cells using the modified guide RNAs.