公开(公告)号：US06797478B1

公开(公告)日：2004-09-28

申请号：US09035708

申请日：1998-03-05

申请人： Frank P. Zemlan ,  Thomas A. Campbell

发明人： Frank P. Zemlan ,  Thomas A. Campbell

IPC分类号： G08N3353

CPC分类号： C07K16/18

摘要： Patients having several neurological diseases have been shown to have elevated levels of axonally-derived proteins (i.e. tau and neurofilament proteins) in cerebrospinal fluid (CSF) and in brain tissue. Three monoclonal antibodies (MAbs) recognizing CSF tau proteins were developed. The Mabs were found to label a ladder of 30 kD to 50 kD tau proteins in CSF from patients with disease states producing axonal damage such as head trauma or CNS tumor but not in CSF from controls. High levels of tau protein in CSF were shown to be diagnostic of axonal degeneration in head trauma. An ELISA assay was developed with these MAbs to aid in the diagnosis of patients with axonal damage.

摘要翻译： 具有几种神经系统疾病的患者已被证明在脑脊液（CSF）和脑组织中具有升高的轴突衍生蛋白（即tau和神经丝蛋白）水平。 开发了识别CSF tau蛋白的三种单克隆抗体（MAb）。 发现Mab在患有CSF产生轴突损伤（例如头部创伤或CNS肿瘤）的患者的CSF中标记30kD至50kD tau蛋白的梯度，而在对照组中的CSF中未标记。 CSF中高水平的tau蛋白显示是头部创伤中轴突变性的诊断。 使用这些MAb开发ELISA测定以帮助诊断具有轴突损伤的患者。