公开(公告)号：US20110293529A1

公开(公告)日：2011-12-01

申请号：US13101370

申请日：2011-05-05

申请人： Tao Ji ,  Warren M. Leevy ,  William E. McLaughlin ,  Yawfui Yong

发明人： Tao Ji ,  Warren M. Leevy ,  William E. McLaughlin ,  Yawfui Yong

IPC分类号： A61K49/00 ,  C12Q1/68 ,  C12Q1/37 ,  G01N33/53 ,  B82Y15/00

CPC分类号： G01N33/587 ,  A61K49/0485 ,  B82Y5/00 ,  B82Y15/00 ,  C12Q1/34 ,  G01N33/542

摘要： Fluorescent activatable probes for imaging biological processes and disease detection in vitro and in vivo. There is also described a method for detecting the presence of an enzyme. A sample is contacted with a nanoparticle including a monomer, at least one cleavable spacer comprising at least one fluorescence activation site, and at least two dyes of at least two types. The cleavable spacer is attached at one end to the nanoparticle. The at least two types comprise an energy donor type and an energy acceptor type. At least one dye of one type is embedded in the nanoparticle and the cleavable spacer has at least one dye of the other type attached. The at least two dyes comprise at least one matched pair capable of fluorescence resonance energy transfer. Fluorescence is detected by exposing the sample to a light source, and detecting emitted light with a detector.

摘要翻译： 用于成像生物过程和体外和体内疾病检测的荧光可激活探针。 还描述了检测酶的存在的方法。 将样品与包含单体的纳米颗粒，至少一个可切割间隔基包含至少一个荧光活化位点和至少两种至少两种类型的染料接触。 可切割间隔物的一端连接到纳米颗粒。 至少两种类型包括能量供体类型和能量受体类型。 至少一种一种染料被包埋在纳米颗粒中，并且可切割间隔物具有至少一种所连接的另一种类型的染料。 所述至少两种染料包含能够进行荧光共振能量转移的至少一个匹配对。 通过将样品暴露于光源并用检测器检测发射光来检测荧光。