公开(公告)号：US06221578B1

公开(公告)日：2001-04-24

申请号：US09117217

申请日：1998-07-24

申请人： Marie-Pierre de Béthune ,  Kurt Hertogs ,  Rudi Pauwels

发明人： Marie-Pierre de Béthune ,  Kurt Hertogs ,  Rudi Pauwels

IPC分类号： C12Q170

CPC分类号： C12N15/86 ,  C12N2740/16011 ,  C12N2740/16211 ,  G01N33/5005 ,  G01N33/5008 ,  G01N33/5014 ,  G01N33/502 ,  G01N33/5044 ,  G01N33/505 ,  Y02A90/22 ,  Y02A90/26

摘要： The present invention is drawn to A method of managing HIV chemotherapy of patients who are HIV positive, which comprises transfecting a cell line susceptible to infection by HIV with a sequence from the pol gene of HIV, which sequence encodes a desired target enzyme, obtained by isolating viral RNA from a sample of a biological material from a patient and reverse transcribing the desired region of the pol gene, and a HIV-DNA construct from which the sequence has been deleted, culturing the transfected cells so as to create a stock of chimeric viruses providing an indication of the resistance profile of the circulating virus, assessing the phenotypic sensitivity of the chimeric viruses to an inhibitor of the enzyme encoded by the pol gene of HIV and assigning a value thereto, constructing a data set comprising the value for chimeric virus sensitivity and the corresponding value for a chimeric wild-type strain of HIV, repeating the sensitivity assessment for at least two further inhibitors and thereby constructing at least three such data sets in total, representing the data sets in two dimensional or three dimensional graphical form such that the difference between the chimeric and wild-type sensitivities in the case of each data set provides a visual measure of the resistance of the chimeric stock to treatment by the inhibitor in question, and selecting the optimum inhibitor(s) on the basis of the graphical representation of the resistance so measured.

摘要翻译： 本发明涉及一种HIV HIV阳性患者的HIV化疗方法，其特征在于：以HIV序列编码易感染HIV的细胞系，所述序列编码所需的靶酶 从患者的生物材料样品中分离病毒RNA并逆转录录pol基因的期望区域，以及从该序列已被缺失的HIV-DNA构建体，培养转染的细胞，从而产生嵌合体 提供循环病毒的抗性谱的指示的病毒，评估嵌合病毒对由HIV的pol基因编码的酶的抑制剂的表型敏感性并赋予其值，构建包含嵌合病毒的值的数据集 敏感性和对应于嵌合野生型HIV株的值，重复进行至少两次进一步抑制的敏感性评估 从而构成总共至少三个这样的数据集，以二维或三维图形形式表示数据集，使得在每个数据集的情况下嵌合和灵敏度之间的差异提供了视觉测量 通过所讨论的抑制剂对嵌合体进行治疗的抗性，并且基于如此测量的电阻的图形表示来选择最佳抑制剂。