公开(公告)号：US06828431B1

公开(公告)日：2004-12-07

申请号：US09699295

申请日：2000-10-26

申请人： Tony N. Frudakis ,  Steven G. Reed ,  John M. Smith ,  Lynda E. Misher ,  Davin C. Dillon ,  Marc W. Retter ,  Aijun Wang ,  Yasir A. W. Skeiky ,  Susan L. Harlocker

发明人： Tony N. Frudakis ,  Steven G. Reed ,  John M. Smith ,  Lynda E. Misher ,  Davin C. Dillon ,  Marc W. Retter ,  Aijun Wang ,  Yasir A. W. Skeiky ,  Susan L. Harlocker

IPC分类号： C12Q170

CPC分类号： C07K14/005 ,  A61K35/12 ,  A61K38/00 ,  A61K39/00 ,  A61K48/00 ,  A61K2039/505 ,  A61K2039/5154 ,  A61K2039/53 ,  A61K2039/57 ,  C07K14/47 ,  C07K14/82 ,  C07K2319/00 ,  C12N2740/11022 ,  C12Q1/6886 ,  C12Q2600/158 ,  Y10S436/813

摘要： Compositions and methods for the therapy and diagnosis of cancer, particularly breast cancer, are disclosed. Illustrative compositions comprise one or more breast tumor polypeptides, immunogenic portions thereof, polynucleotides that encode such polypeptides, antigen presenting cell that expresses such polypeptides, and T cells that are specific for cells expressing such polypeptides. The disclosed compositions are useful, for example, in the diagnosis, prevention and/or treatment of diseases, particularly breast cancer.

公开(公告)号：US06811970B2

公开(公告)日：2004-11-02

申请号：US10205641

申请日：2002-07-25

申请人： Nicolas Sluis-Cremer ,  Michael Parniak ,  Alex Pelletier

发明人： Nicolas Sluis-Cremer ,  Michael Parniak ,  Alex Pelletier

IPC分类号： C12Q170

CPC分类号： C12Q1/48 ,  G01N2333/9125 ,  G01N2500/02

摘要： A method for measuring heterodimerization of HIV RT, which comprises the steps of: a) providing a first solution comprising p66 subunit homodimers in the presence of a dissociation agent; b) contacting the first solution with p51 RT subunits and incubating in the presence of a reassociation buffer to allow association of a complex of p66/p51 RT subunits, wherein one of the subunits comprises an affinity tag and the other of the subunits comprises a detectable label; c) contacting the incubate of step b) with an affinity medium under conditions that enable the p66/p51 complex to bind to the affinity medium; and d) determining the amount of complex formed by measuring the level of detectable label bound to the affinity medium (or by measuring the reconstituted RT polymerase activity). Steps a) to d) can be carried out in the presence or absence of a test compound followed by e) comparing the test compound sample to a control sample lacking the compound, whereby modulated p66/p51 complex formation in the test compound sample is indicative of the ability of the compound to modulate, inhibit or enhance heterodimerization. The method can be used to screen for inhibitors of HIV RT dimerization.

摘要翻译： 一种用于测量HIV RT的异源二聚化的方法，其包括以下步骤：a）在解离剂存在下提供包含p66亚基同源二聚体的第一溶液; b）使第一溶液与p51RT亚基接触，并在再结合缓冲液存在下孵育以允许p66 / p51 RT亚基的复合物缔合，其中一个亚基包含亲和标签，另一个亚基包含可检测的 标签; c）使能使p66 / p51复合物与亲和介质结合的条件下使步骤b）的培养物与亲和介质接触; 和d）通过测量与亲和介质结合的可检测标记的水平（或通过测量重构的RT聚合酶活性）来确定形成的复合物的量。 步骤a）至d）可以在存在或不存在测试化合物的情况下进行，随后e）将测试化合物样品与缺少化合物的对照样品进行比较，由此在测试化合物样品中调节的p66 / p51复合体形成是指示性的 的化合物调节，抑制或增强异源二聚化的能力。 该方法可用于筛选HIV RT二聚化抑制剂。

公开(公告)号：US06797464B2

公开(公告)日：2004-09-28

申请号：US10192369

申请日：2002-07-10

申请人： Mario Stevenson ,  Mark Sharkey

发明人： Mario Stevenson ,  Mark Sharkey

IPC分类号： C12Q170

CPC分类号： C12Q1/703

摘要： The invention relates to methods of detecting a drug resistant HIV in a subject. The methods include detecting mutations associated with drug resistance in an HIV 2-LTR circle DNA molecule obtained from a cell of an HIV-positive subject, e.g, an HIV-1-positive human.

摘要翻译： 本发明涉及检测受试者中耐药性HIV的方法。 所述方法包括检测从HIV阳性受试者（例如HIV-1阳性人）的细胞获得的HIV2-LTR圈DNA分子中与耐药性相关的突变。

公开(公告)号：US06797463B2

公开(公告)日：2004-09-28

申请号：US09784232

申请日：2001-02-15

申请人： Nicholas L. Abbott ,  Justin J. Skaife

发明人： Nicholas L. Abbott ,  Justin J. Skaife

IPC分类号： C12Q170

CPC分类号： B82Y30/00 ,  G01N33/54373 ,  G01N33/569 ,  G01N33/56911 ,  Y10S436/807

摘要： Detection apparatus for use in the detection of the presence of a selected pathogen in a sample are disclosed. Such apparatus include: a substrate with a detection region on a surface thereof, the detection region having microstructures including grooves formed therein that will align liquid crystal material in contact therewith, the width and depth of the grooves being in the range of 10 &mgr;m or less; a blocking layer on the surface of the detection region of the substrate that does not disrupt the alignment of liquid crystal material in contact therewith, the blocking layer blocking nonspecific adsorption of pathogens to the surface; and a binding agent on the surface of the detection region of the substrate, the binding agent specifically binding the selected pathogen.

摘要翻译： 公开了用于检测样品中选定病原体的存在的检测装置。 这种装置包括：具有在其表面上具有检测区域的基板，检测区域具有包括形成在其中的凹槽的微结构，其将与其接触的液晶材料对准，凹槽的宽度和深度在10um或更小的范围内 ; 在基板的检测区域的表面上的阻挡层不会破坏与其接触的液晶材料的取向，阻挡层阻止病原体向表面的非特异性吸附; 和底物的检测区域的表面上的结合剂，所述结合剂特异性结合所选择的病原体。

公开(公告)号：US06790612B2

公开(公告)日：2004-09-14

申请号：US10191966

申请日：2002-07-10

申请人： Karen Elizabeth Potts ,  Roberta Lynn Jackson ,  Amy Karen Patick

发明人： Karen Elizabeth Potts ,  Roberta Lynn Jackson ,  Amy Karen Patick

IPC分类号： C12Q170

CPC分类号： G01N33/502 ,  C07K14/005 ,  C12N2770/24222 ,  C12Q1/37 ,  C12Q1/42 ,  C12Q1/706 ,  G01N33/5008 ,  G01N2333/18

摘要： A cell-based assay system in which the detection of the reporter gene activity, or secreted alkaline phosphatase (SEAP), is dependent upon the protease activity of the Hepatitis C virus NS3 gene product. This system can be used to assess the activity of candidate protease inhibitors in a mammalian cell-based assay system. The assay system is simpler than previously described assays due to the use of SEAP which allows the reporter gene activity to be quantified by measuring the amount of secreted gene product in the cell media by monitoring the conversion of luminescent or calorimetric alkaline phosphatase substrate.

摘要翻译： 检测报告基因活性或分泌的碱性磷酸酶（SEAP）的基于细胞的测定系统取决于丙型肝炎病毒NS3基因产物的蛋白酶活性。 该系统可用于评估基于哺乳动物细胞的测定系统中候选蛋白酶抑制剂的活性。 由于使用SEAP，测定系统比以前描述的测定更简单，其允许通过监测发光或量热碱性磷酸酶底物的转化来测量细胞培养基中分泌的基因产物的量来定量报道基因活性。

公开(公告)号：US06777178B1

公开(公告)日：2004-08-17

申请号：US09763625

申请日：2001-06-04

申请人： Yoko Aida

发明人： Yoko Aida

IPC分类号： C12Q170

CPC分类号： C07K16/2833

摘要： A c143 monoclonal antibody which is used for detecting a bovine individual having a possibility of onset of bovine leukemia; a monoclonal antibody which is used for detecting a bovine individual having a possibility of onset of bovine leukemia, wherein the monoclonal antibody has the substantially same reactivity as the c143 monoclonal antibody to a bovine MHC Class II DR molecule to which a possibility of onset of bovine leukemia is attributable; and an agent for diagnosing a possibility of onset of bovine leukemia which comprises the aforementioned monoclonal antibody. Bovine individuals having a possibility of onset of bovine leukemia can be conveniently and accurately detected.

摘要翻译： 用于检测具有牛白血病发病可能性的牛个体的c143单克隆抗体; 用于检测具有牛白血病发病可能性的牛个体的单克隆抗体，其中所述单克隆抗体与具有牛发病可能性的牛MHC II类DR分子具有与c143单克隆抗体基本相同的反应性 白血病归因于 以及用于诊断包含上述单克隆抗体的牛白血病发病可能性的药剂。 可以方便，准确地检测出具有牛白血病发生可能性的牛个体。

公开(公告)号：US06713251B2

公开(公告)日：2004-03-30

申请号：US09943983

申请日：2001-08-31

申请人： Lieven Stuyver ,  Joost Louwagie ,  Rudi Rossau

发明人： Lieven Stuyver ,  Joost Louwagie ,  Rudi Rossau

IPC分类号： C12Q170

CPC分类号： C12Q1/703

摘要： The present invention relates to a method for the rapid and reliable detection of drug-induced mutations in the reverse transcriptase gene allowing the simultaneous characterization of a range of codons involved in drug resistance using specific sets of probes optimized to function together in a reverse-hybridization assay. More particularly, the present invention relates to a method for determining the susceptibility to antiviral drugs of HIV strains present in a biological sample, comprising: (i) if need be releasing, isolating or concentrating the polynucleic acids present in the sample; (ii) if need be amplifying the relevant part of the reverse transcriptase genes present in said sample with at least one suitable primer pair; (iii) hybridizing the polynucleic acids of step (i) or (ii) with at least two RT gene probes hybridizing specifically to one or more target sequences with said probes being applied to known locations on a solid support and with said probes being capable of simultaneously hybridizing to their respective target regions under appropriate hybridization and wash conditions allowing the detection of homologous targets, or said probes hybridizing specifically with a sequence complementary to any of said target sequences, or a sequence wherein T is replaced by U; (iv) detecting the hybrids formed in step (iii); (v) inferring the nucleotide sequence at the codons of interest and/or the amino acids of the codons of interest and/or antiviral drug resistance spectrum, and possibly the type of HIV isolates involved from the differential hybridization signal(s) obtained in step (iv).

摘要翻译： 本发明涉及用于快速和可靠地检测逆转录酶基因中的药物诱导突变的方法，其允许使用经优化以在反向杂交中一起起作用的特定的探针组来同时表征涉及耐药性的一系列密码子 测定。 更具体地说，本发明涉及确定存在于生物样品中的HIV病毒抗病毒药物易感性的方法，其包括：（i）如果需要释放，分离或浓缩样品中存在的多核酸; （ii）如果需要用至少一个合适的引物对扩增所述样品中存在的逆转录酶基因的相关部分; （iii）将步骤（i）或（ii）中的多核酸与至少两个与一个或多个靶序列特异性杂交的RT基因探针杂交，所述探针被施加到固体支持物上的已知位置，并且所述探针能够 在适当的杂交和洗涤条件下同时与其各自的靶区域杂交，允许检测同源靶标，或所述探针与与任何所述靶序列互补的序列特异性杂交，或其中T被U替代的序列; （iv）检测步骤（iii）中形成的杂交体; （v）推断感兴趣的密码子和/或感兴趣的密码子的氨基酸和/或抗病毒药物抗性谱的核苷酸序列，以及可能的步骤中获得的差异杂交信号所涉及的HIV分离物的类型 （iv）。

公开(公告)号：USRE38442E1

公开(公告)日：2004-02-24

申请号：US09798641

申请日：2001-03-02

申请人： David Y. Zhang ,  Margaret Brandwein

发明人： David Y. Zhang ,  Margaret Brandwein

IPC分类号： C12Q170

CPC分类号： C12Q1/6862 ,  C12Q1/6813 ,  C12Q1/682 ,  C12Q1/6834 ,  C12Q1/6844 ,  C12Q1/6853 ,  C12Q1/686 ,  C12Q1/6865 ,  G01N33/536 ,  C12Q2563/131 ,  C12Q2563/143 ,  C12Q2565/519

摘要： An improved method allowing for rapid sensitive and standardized detection of a target nucleic acid from a pathogenic microorganism or virus or normal or abnormal gene in a sample is provided. The method involves hybridizing a target nucleic acid to several non-overlapping oligonucleotide probes that hybridize to adjacent regions in the target nucleic acid, the probes being referred to capture/amplification probes and amplifications probes, respectively, in the presence of paramagnetic beads coated with a ligand binding moiety. Through the binding of a ligand attached to one end of the capture/amplification probe and the specific hybridization of portions of the probes to adjacent sequences in the target nucleic acid, a complex comprising the target nucleic acid, the probes and the paramagnetic beads is formed. The probes may then ligated together to form a contiguous ligated amplification sequence bound to the beads, which complex may be denatured to remove the target nucleic acid and unligated probes. Alternatively, separate capture and amplification probes may be used which form continuous full-length or circular probes, and may be directly detected or amplified using a suitable amplification technique, e.g., PCR, RAM or HSAM for detection. The detection of the ligated amplification sequence, either directly or following amplification of the ligated amplification sequence, indicates the presence of the target nucleic acid in a sample. Methods for the detection of the ligated amplification sequence, including hybridization signal amplification method and ramification- extension amplification method, are also provided.

公开(公告)号：US06696242B1

公开(公告)日：2004-02-24

申请号：US08470246

申请日：1995-06-06

申请人： Sergei A. Tsarev ,  Suzanne U. Emerson ,  Robert H. Purcell

发明人： Sergei A. Tsarev ,  Suzanne U. Emerson ,  Robert H. Purcell

IPC分类号： C12Q170

CPC分类号： C12N15/1131 ,  A61K38/00 ,  A61K39/00 ,  C07H21/00 ,  C07K14/005 ,  C12N7/00 ,  C12N2310/3125 ,  C12N2310/314 ,  C12N2310/315 ,  C12N2310/321 ,  C12N2310/341 ,  C12N2310/345 ,  C12N2310/346 ,  C12N2710/14143 ,  C12N2770/28122 ,  C12N2770/28123 ,  Y10S436/82 ,  C12N2310/3521

摘要： A strain of hepatitis E virus from Pakistan (SAR-55) implicated in an epidemic of enterically transmitted non-A, non-B hepatitis, now called hepatitis E, is disclosed. The invention relates to the expression of the whole structural region of SAR-55, designated open reading frame 2 (ORF-2), in a eukaryotic expression system. The expressed protein is capable of forming HEV virus-like particles which can serve as an antigen in diagnostic immunoassays and as an immunogen or vaccine to protect against infection by hepatitis E.

摘要翻译： 披露了一种来自巴基斯坦的丙型肝炎病毒（SAR-55），涉及到目前被称为戊型肝炎的非传染性非乙型肝炎的流行病。 本发明涉及真核表达系统中SAR-55整体结构域的表达，其命名为开放阅读框架2（ORF-2）。 表达的蛋白质能够形成HEV病毒样颗粒，其可以在诊断免疫测定中作为抗原，并作为免疫原或疫苗以防止乙型肝炎感染。

公开(公告)号：US06689558B2

公开(公告)日：2004-02-10

申请号：US09779233

申请日：2001-02-08

申请人： Casey Case

发明人： Casey Case

IPC分类号： C12Q170

CPC分类号： G01N33/502 ,  G01N33/5008 ,  G01N33/5023 ,  G01N33/5041 ,  G01N33/6872 ,  G01N2500/00

摘要： Disclosed herein are compositions and method useful in screening a compound for its interaction and/or effect with a molecular target and/or cellular process.

摘要翻译： 本文公开了可用于筛选化合物用于分子靶和/或细胞过程的相互作用和/或效应的组合物和方法。