公开(公告)号：US20100028955A1

公开(公告)日：2010-02-04

申请号：US12507743

申请日：2009-07-22

申请人： Kai Qin Lao ,  Neil Straus

发明人： Kai Qin Lao ,  Neil Straus

IPC分类号： C12P19/34 ,  C12N9/00

CPC分类号： C12Q1/6853 ,  C12Q2537/143 ,  C12Q2531/119 ,  C12Q2525/15

摘要： The present disclosure relates to the amplification of target nucleic acid sequences for various sequencing and/or identification techniques. This can be accomplished via the use of target primers and isothermal multiple strand displacement (MDA) processes. The use of these target primers and MDA, as described herein, allows for, for example, the reduction in the amplification of undesired hybridization events (such as primer dimerization and the “jackpot mutation” effect of PCR) while allowing for the amplification of the target nucleic acid sequences.

摘要翻译： 本公开涉及用于各种测序和/或鉴定技术的靶核酸序列的扩增。 这可以通过使用目标引物和等温多链置换（MDA）过程来实现。 如本文所述，这些靶引物和MDA的使用允许例如减少不想要的杂交事件（例如引物二聚化和PCR的“累积突变”效应）的扩增，同时允许扩增 靶核酸序列。

公开(公告)号：US20080161197A1

公开(公告)日：2008-07-03

申请号：US11955197

申请日：2007-12-12

申请人： Kai Qin Lao

发明人： Kai Qin Lao

IPC分类号： C40B30/00 ,  C12P19/34

CPC分类号： C12P19/34 ,  C12Q1/6848 ,  C12Q2521/107 ,  C12Q2525/155 ,  C12Q2525/173

摘要： The present teachings provide methods for amplifying a plurality of target nucleic acids. In some embodiments, a first oligo-dT-universal primer comprising a 3′ oligo-dT portion and a first 5′ universal portion is used to reverse transcribe a plurality of 3′ poly-A tail-containing nucleic acids. A poly-A tail is added to the 3′ end of the first strand products to form a two-tailed reaction product. The two-tailed reaction product is amplified in a PCR, wherein the PCR comprises the first oligo-dT-universal primer, and a second oligo-dT-universal primer, wherein the second oligo-dT-universal primer comprises a 3′ oligo-dT portion and a second 5′ universal portion, wherein the second 5′ universal portion of the second oligo-dT-universal primer comprises a different sequence than the first 5′ universal portion of the first oligo-dT-universal primer. The present teachings also provide compositions and kits for amplifying target nucleic acids containing monomorphic tails.

摘要翻译： 本教导提供了扩增多个靶核酸的方法。 在一些实施方案中，使用包含3'oligo-dT部分和第一个5'通用部分的第一寡聚-DT通用引物逆转录数多个含3'多聚氨基酸的核酸。 将poly-A尾添加到第一链产物的3'末端以形成双尾反应产物。 双链反应产物在PCR中扩增，其中PCR包含第一寡聚-D-通用引物和第二寡聚-DT通用引物，其中第二寡聚-D-通用引物包含3'寡聚 - dT部分和第二5'通用部分，其中第二寡聚-D-通用引物的第二个5'通用部分包含与第一寡聚-DT通用引物的前5'通用部分不同的序列。 本教导还提供用于扩增含有单形尾的靶核酸的组合物和试剂盒。