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    COMPOSITIONS AND TECHNIQUES FOR NUCLEIC ACID PRIMER EXTENSION
    2.
    发明申请
    COMPOSITIONS AND TECHNIQUES FOR NUCLEIC ACID PRIMER EXTENSION 审中-公开

    公开(公告)号：US20190241945A1

    公开(公告)日：2019-08-08

    申请号：US16265942

    申请日：2019-02-01

    申请人： OMNIOME, INC.

    发明人： Denis Malyshev ,  Arnold Oliphant

    IPC分类号： C12Q1/6853

    CPC分类号： C12Q1/6853

    摘要： A method of characterizing a nucleic acid that includes steps of (a) contacting a primer-template nucleic acid hybrid with a polymerase and a mixture of nucleotides to produce an extended primer hybrid and to form a stabilized ternary complex including the extended primer hybrid, the polymerase and a nucleotide cognate of the next base in the template, wherein the mixture contains nucleotide cognates of four different base types, wherein the nucleotide cognate of the first base type has a reversible terminator, and wherein nucleotide cognates of the second, third and fourth base types are extendable; (b) detecting the stabilized ternary complex to distinguish the next base from other base types in the template; and (c) determining the presence of a base multiplet in the template nucleic acid, the base multiplet including the first base type followed by the next base.

    NUCLEIC ACID OLIGOMER FOR RNA HYBRID FORMATION
    6.
    发明申请
    NUCLEIC ACID OLIGOMER FOR RNA HYBRID FORMATION 审中-公开

    公开(公告)号：US20190194746A1

    公开(公告)日：2019-06-27

    申请号：US16083347

    申请日：2017-03-09

    申请人： TOKYO UNIVERSITY OF SCIENCE FOUNDATION

    发明人： Takeshi Wada ,  Reijiro Yoshino ,  Rintaro Hara ,  Yohei Nukaga

    IPC分类号： C12Q1/6876 ,  C12Q1/6853 ,  C07H21/04

    CPC分类号： C12Q1/6876 ,  A61K31/7088 ,  A61K48/00 ,  C07H21/04 ,  C12N15/00 ,  C12N15/113 ,  C12Q1/6853 ,  C12Q2527/101 ,  C12Q2527/107 ,  C12Q2600/166

    摘要： A nucleic acid oligomer for RNA hybrid formation, including a structure formed of from 3 to 50 continuous units of at least one unit of a nucleotide unit represented by the following General Formula (1) or a nucleotide unit represented by the following General Formula (2); and having a base length of from 8 bases to 50 bases: in which in the formulae, R1 represents a hydrogen atom, an alkoxy group, an alkenyloxy group, an acyloxy group, a trialkylsilyloxy group, or a halogenyl group, and, in the formulae, each Bs1 independently represents a pyrimidine base that may have a protecting group, a hydrogen atom bonded to a carbon atom at the 5-position of the pyrimidine base may be substituted with a group other than a hydrogen atom, in the formulae, each X independently represents S−Z+ or BH3—Z+, Z+ represents a counter cation, each R2 and R3 independently represents a hydrogen atom, or an alkyl group having from 1 to 10 carbon atoms, and R2 and R3 may be bonded to each other to form a ring.

    PROCESS FOR THE AMPLIFICATION OF NUCLEIC ACIDS AND USE OF A KIT FOR CARRYING OUT THE SAME
    7.
    发明申请
    PROCESS FOR THE AMPLIFICATION OF NUCLEIC ACIDS AND USE OF A KIT FOR CARRYING OUT THE SAME 审中-公开

    公开(公告)号：US20190185914A1

    公开(公告)日：2019-06-20

    申请号：US16328141

    申请日：2017-08-21

    申请人： AGCT GmbH

    发明人： Dmitry CHERKASOV ,  Norbert BASLER ,  Claus BECKER ,  Hans-Joerg HESS ,  Andreas MUELLER-HERMANN

    IPC分类号： C12Q1/686 ,  C12Q1/6806 ,  C12Q1/6853 ,  C12Q1/6876

    CPC分类号： C12Q1/686 ,  C12Q1/6806 ,  C12Q1/6848 ,  C12Q1/6853 ,  C12Q1/6876 ,  C12Q2525/186 ,  C12Q2531/119 ,  C12Q2537/1373 ,  C12Q2525/155 ,  C12Q2537/162

    摘要： It is disclosed a method for amplification of nucleic acids in which substantially use is made of the fact that a pre-defined nucleic acid chain (target sequence) can be multiplied/amplified in the presence of a target sequence-specific activator oligonucleotide. The target sequence-specific activator oligonucleotide causes the separation of re-synthesized complementary primer extension products by means of strand displacement, so that a new primer oligonucleotide can attach to the respective template strand. The thus formed complex of a primer oligonucleotide and a template strand can initiate a new primer extension reaction. The thus formed primer extension products in turn function as templates, so that an exponential amplification reaction results.Here, the activator oligonucleotide itself does not function as a template and preferably is inert over a primer extension reaction. The activator oligonucleotide functions as a co-factor in the reaction that regenerates the single-stranded state of the relevant strand segments of the templates needed for the reaction.By the use of a set of components comprising at least one primer oligonucleotide, at least one polymerase, a set of substrates for polymerase (e.g., dNTPs), and at least one specific activator oligonucleotide a nucleic acid to be amplified can be amplified linearly.By the use of such a set and a second primer oligonucleotide a nucleic acid to be amplified can be amplified exponentially, wherein the resulting specific primer extension products are used for further primer extension reactions as templates.