公开(公告)号：US20240318233A1

公开(公告)日：2024-09-26

申请号：US18394469

申请日：2023-12-22

Applicant: LUMINEX CORPORATION

Inventor： Douglas F. WHITMAN ,  Hongwei ZHANG

IPC: C12Q1/6818 ,  C12Q1/68 ,  C12Q1/6813 ,  C12Q1/6816 ,  C12Q1/6823 ,  C12Q1/6834 ,  C12Q1/6853 ,  C12Q1/6876

CPC classification number: C12Q1/6818 ,  C12Q1/68 ,  C12Q1/6813 ,  C12Q1/6816 ,  C12Q1/6823 ,  C12Q1/6834 ,  C12Q1/6853 ,  C12Q1/6876

Abstract: Methods and compositions for nucleic acid amplification, detection, and genotyping techniques are disclosed. In one embodiment, a nucleic acid molecule having a target-specific primer sequence; an anti-tag sequence 5′ of the target-specific primer sequence; a tag sequence 5′ of the anti-tag sequence; and a blocker between the anti-tag sequence and the tag sequence is disclosed. Compositions containing such a nucleic acid molecule and methods of using such a nucleic acid molecule are also disclosed.

公开(公告)号：US20240318231A1

公开(公告)日：2024-09-26

申请号：US18442644

申请日：2024-02-15

Applicant: Gen-Probe Incorporated

Inventor： Mehrdad MAJLESSI ,  Pamela DOUGLASS ,  Daniel KOLK

IPC: C12Q1/6811 ,  C12Q1/6816 ,  C12Q1/6851 ,  C12Q1/6853 ,  C12Q1/686 ,  C12Q1/6876 ,  C12Q1/6888 ,  C12Q1/70

CPC classification number: C12Q1/6811 ,  C12Q1/6816 ,  C12Q1/6851 ,  C12Q1/6853 ,  C12Q1/686 ,  C12Q1/6876 ,  C12Q1/6888 ,  C12Q1/701 ,  C12Q2600/118 ,  C12Q2600/166

Abstract: Compositions, methods, kits, and uses are provided for detecting or quantifying an Human Parainfluenza virus 1 (HPIV-1), HPIV-2, HPIV-3, and/or HPIV-4 nucleic acid, e.g., using nucleic acid amplification and hybridization assays. In some embodiments, the compositions, methods, kits, and uses target the HN gene of HPIV-1, HPIV-2, and/or HPIV-3 and/or the NP gene of HPIV-4.

公开(公告)号：US20240301482A1

公开(公告)日：2024-09-12

申请号：US18441502

申请日：2024-02-14

Applicant: Natera, Inc.

Inventor： Huseyin Eser KIRKIZLAR ,  Raheleh SALARI ,  Stymir SIGURJONSSON ,  Bernhard ZIMMERMANN ,  Allison RYAN ,  Naresh VANKAYALAPATI

IPC: C12Q1/6858 ,  C12Q1/6853 ,  C12Q1/6869 ,  C12Q1/6886 ,  G16B20/00 ,  G16B20/10 ,  G16H10/40

CPC classification number: C12Q1/6858 ,  C12Q1/6853 ,  C12Q1/6869 ,  C12Q1/6886 ,  G16B20/00 ,  G16B20/10 ,  G16H10/40 ,  C12Q2539/10 ,  C12Q2600/106 ,  C12Q2600/156 ,  Y02A90/10

Abstract: The invention provides improved methods, compositions, and kits for detecting ploidy of chromosome regions, e.g. for detecting cancer or a chromosomal abnormality in a gestating fetus. The methods can utilize a set of more than 200 SNPs that are found within haploblocks and can include analyzing a series of target chromosomal regions related to cancer or a chromosomal abnormality in a gestating fetus. Finally the method may use knowledge about chromosome crossover locations or a best fit algorithm for the analysis. The compositions may comprise more than 200 primers located within haplotype blocks known to show CNV.

公开(公告)号：US12077808B2

公开(公告)日：2024-09-03

申请号：US17735509

申请日：2022-05-03

Applicant: The University of North Carolina at Chapel Hill

Inventor： John Michael Ramsey ,  William Henley ,  Emily Oblath

IPC: C12Q1/68 ,  B01L3/00 ,  B01L7/00 ,  C12Q1/6823 ,  C12Q1/6853 ,  C12Q1/686 ,  G01N35/00

CPC classification number: C12Q1/6823 ,  B01L3/502761 ,  B01L7/52 ,  C12Q1/6853 ,  C12Q1/686 ,  B01J2219/00317 ,  B01J2219/00454 ,  B01J2219/00495 ,  B01J2219/005 ,  B01J2219/00554 ,  B01J2219/00576 ,  B01J2219/00619 ,  B01L3/5027 ,  B01L3/502715 ,  B01L2200/16 ,  B01L2300/0636 ,  B01L2300/0829 ,  B01L2300/0864 ,  G01N2035/00158 ,  C12Q1/6853 ,  C12Q2563/149 ,  C12Q2565/629 ,  C12Q1/6823 ,  C12Q2537/143 ,  C12Q2563/149 ,  C12Q2565/537 ,  C12Q2565/629

Abstract: A microfluidic device includes a plurality of reaction wells; and a plurality of solid supports, and each of the solid supports has a reagent attached thereto. The reagent is attached to the solid support via a labile reagent/support bond such that the reagent is configured to be cleaved from the support via a cleaving operation.

公开(公告)号：US12071672B2

公开(公告)日：2024-08-27

申请号：US17474174

申请日：2021-09-14

Applicant: Wisconsin Alumni Research Foundation

Inventor： David Frazier Jarrard ,  Bing Yang

IPC: C12Q1/68 ,  C12Q1/6806 ,  C12Q1/6853 ,  C12Q1/686 ,  C12Q1/6886

CPC classification number: C12Q1/6886 ,  C12Q1/6806 ,  C12Q1/6853 ,  C12Q1/686 ,  C12Q2337/00 ,  C12Q2600/106 ,  C12Q2600/112 ,  C12Q2600/118 ,  C12Q2600/154 ,  C12Q2600/158

Abstract: A method of detecting the presence of a prostate cancer field defect in a human subject. The method includes (a) obtaining genomic DNA from the human subject and (b) determining methylation status in at least one target region selected from the group consisting of PLA2G16, CAV1, EVX1, MCF2L, FGF1, NCR2, WNT2, EXT1, and SPAG4, wherein methylation changes indicate the presence of prostate cancer or a prostate cancer field defect, wherein the change is relative to tissue from a second human subject who does not have prostate cancer.

公开(公告)号：US20240279728A1

公开(公告)日：2024-08-22

申请号：US18568668

申请日：2022-06-08

Applicant: UTI Limited Partnership

Inventor： Pierre BILLON ,  Lou Anne Ghyslaine BAUDRIER ,  Orlena Aviela Camille BENAMOZIG

IPC: C12Q1/6853

CPC classification number: C12Q1/6853

Abstract: The present disclosure relates to improvements in the Dinucleotide signaTurE CapTure (DTECT) method. The improved detection of a dinucleotide sequence in a target polynucleotide generally involves the steps of Acu1 digestion, heat inactivation and ligation to unique adaptors containing overhangs of two bases complementary to the dinucleotide signature.

公开(公告)号：US12049671B2

公开(公告)日：2024-07-30

申请号：US17409132

申请日：2021-08-23

Applicant: EXACT SCIENCES CORPORATION ,  Mayo Foundation for Medical Education and Research

Inventor： Hatim T. Allawi ,  Michael W. Kaiser ,  Graham P. Lidgard ,  William R. Taylor ,  Tamara J. Sander ,  Abram M. Vaccaro

IPC: C12Q1/6883 ,  C12Q1/6809 ,  C12Q1/6827 ,  C12Q1/6853 ,  C12Q1/6886 ,  G01N33/533 ,  G01N33/574 ,  G16Z99/00

CPC classification number: C12Q1/6883 ,  C12Q1/6809 ,  C12Q1/6827 ,  C12Q1/6853 ,  C12Q1/6886 ,  G01N33/533 ,  G01N33/57419 ,  G16Z99/00 ,  C12Q2600/106 ,  C12Q2600/112 ,  C12Q2600/118 ,  C12Q2600/154 ,  C12Q2600/156 ,  C12Q2600/16

Abstract: Provided herein is technology for neoplasia screening, and particularly, but not exclusively, to methods, compositions, and related uses for detecting the presence of cancer, in particular, colorectal cancer.

公开(公告)号：US20240191306A1

公开(公告)日：2024-06-13

申请号：US18430156

申请日：2024-02-01

Applicant: Case Western Reserve University

Inventor： Sanford D. Markowitz ,  Helen Moinova ,  Amitabh Chak ,  Joseph Willis ,  Thomas LaFramboise

IPC: C12Q1/6886 ,  C12Q1/6827 ,  C12Q1/6853 ,  G01N33/574

CPC classification number: C12Q1/6886 ,  C12Q1/6827 ,  C12Q1/6853 ,  G01N33/574 ,  C12Q2600/154 ,  C12Q2600/156

Abstract: The disclosure provides methods for identifying genomic loci (e.g., vimentin and/or SqBE18) that are differentially methylated in metaplasias (e.g., Barrett's esophagus) and/or neoplastic cancers (e.g., esophageal cancers). Identification of methylated genomic loci has numerous uses, including for example, to characterize disease risk, to predict responsiveness to therapy, to non-invasively diagnose subjects and to treat subjects determined to have gastrointestinal metaplasias and/or neoplasias.

公开(公告)号：US20240124926A1

公开(公告)日：2024-04-18

申请号：US18531603

申请日：2023-12-06

Applicant: Pacific Biosciences of California, Inc.

Inventor： Arnold Oliphant ,  Denis Malyshev

IPC: C12Q1/6853 ,  C12Q1/6869

CPC classification number: C12Q1/6853 ,  C12Q1/6869

Abstract: A method of characterizing a nucleic acid that includes steps of (a) contacting a primer-template nucleic acid hybrid with a polymerase and a mixture of nucleotides to produce an extended primer hybrid and to form a stabilized ternary complex including the extended primer hybrid, the polymerase and a nucleotide cognate of the next base in the template, wherein the mixture contains nucleotide cognates of four different base types, wherein the nucleotide cognate of the first base type has a reversible terminator, and wherein nucleotide cognates of the second, third and fourth base types are extendable; (b) detecting the stabilized ternary complex to distinguish the next base from other base types in the template; and (c) determining the presence of a base multiplet in the template nucleic acid, the base multiplet including the first base type followed by the next base.

公开(公告)号：US20240093294A1

公开(公告)日：2024-03-21

申请号：US18520654

申请日：2023-11-28

Applicant: President and Fellows of Harvard College

Inventor： Xiaoliang Sunney Xie ,  Katsuyuki Shiroguchi ,  Peter A. Sims ,  Tony Z. Jia

IPC: C12Q1/6874 ,  C12N15/10 ,  C12Q1/6853

CPC classification number: C12Q1/6874 ,  C12N15/1065 ,  C12Q1/6853 ,  C12N2320/10

Abstract: Methods and compositions for digital profiling of nucleic acid sequences present in a sample are provided.