公开(公告)号：US10858682B2

公开(公告)日：2020-12-08

申请号：US16195608

申请日：2018-11-19

Applicant: DSM IP ASSETS B.V.

Inventor： Johannes Petrus Smits ,  Michael Petrus Jozef Berkhout ,  Bertus Noordam

IPC: C12P19/02 ,  C12P19/14 ,  C13K1/02

Abstract: The invention relates to a process for the hydrolysis of cellulose containing biomass which comprises a liquefaction step in which a first enzyme or first enzyme composition is added to liquefy at least part of the solids present in the biomass and to keep the viscosity of the cellulose containing biomass below 1000 cP, preferably below 800 cP, more preferably below 600 cP in the liquefaction step; followed by a saccharification step in which a second enzyme composition is added to form oligomeric and/or monomeric sugars; and whereby the first enzyme or first enzyme composition is different from the second enzyme composition; whereby the first enzyme or first enzyme composition comprises an endoglucanase; whereby the second enzyme composition comprises a cellulase; and whereby the first enzyme or first enzyme composition comprises more endoglucanase than the second enzyme composition (expressed in protein wt %).

公开(公告)号：US10724057B2

公开(公告)日：2020-07-28

申请号：US15934636

申请日：2018-03-23

Applicant: DSM IP ASSETS B.V.

Inventor： Bertus Noordam ,  Michael Petrus Jozef Berkhout ,  Aida Hiseni

IPC: C12P7/10 ,  C12P7/16 ,  C12P7/18 ,  C12P7/20 ,  C12P7/40 ,  C12P7/42 ,  C12P7/46 ,  C12P7/48 ,  C12P7/54 ,  C12P7/56 ,  C12P17/10 ,  C12P19/02 ,  C12P19/14 ,  C12P35/00 ,  C12P5/02

Abstract: The invention relates to a process for the preparation of a sugar product from ligno-cellulosic material, comprising the following steps: a) optionally pre-treatment of the ligno-cellulosic material; b) optionally washing of the optionally pre-treated ligno-cellulosic material; c) enzymatic hydrolysis of the optionally washed and/or optionally pre-treated ligno-cellulosic material using an enzyme composition comprising at least two cellulase and whereby the enzyme composition at least comprises GH61; and d) optionally recovery of a sugar product; wherein during part of the time of the enzymatic hydrolysis, oxygen is added to the ligno-cellulosic material and during part of the time of the enzymatic hydrolysis less oxygen is added to the ligno-cellulosic material compared to the other part of the time of the enzymatic hydrolysis, preferably no oxygen is added to the ligno-cellulosic material.

公开(公告)号：US20190085366A1

公开(公告)日：2019-03-21

申请号：US16195608

申请日：2018-11-19

Applicant: DSM IP ASSETS B.V.

Inventor： Johannes Petrus Smits ,  Michael Petrus Jozef Berkhout ,  Bertus Noordam

IPC: C12P19/02 ,  C12P19/14 ,  C13K1/02

Abstract: The invention relates to a process for the hydrolysis of cellulose containing biomass which comprises a liquefaction step in which a first enzyme or first enzyme composition is added to liquefy at least part of the solids present in the biomass and to keep the viscosity of the cellulose containing biomass below 1000 cP, preferably below 800 cP, more preferably below 600 cP in the liquefaction step; followed by a saccharification step in which a second enzyme composition is added to form oligomeric and/or monomeric sugars; and whereby the first enzyme or first enzyme composition is different from the second enzyme composition; whereby the first enzyme or first enzyme composition comprises an endoglucanase; whereby the second enzyme composition comprises a cellulase; and whereby the first enzyme or first enzyme composition comprises more endogluconase than the second enzyme composition (expressed in protein wt %).

公开(公告)号：US10174351B2

公开(公告)日：2019-01-08

申请号：US14655349

申请日：2014-01-09

Applicant: DSM IP ASSETS B.V.

Inventor： Johannes Petrus Smits ,  Michael Petrus Jozef Berkhout ,  Bertus Noordam

IPC: C12P19/02 ,  C12P19/14 ,  C13K1/02

Abstract: The invention relates to a process for the hydrolysis of cellulose containing biomass which comprises a liquefaction step in which a first enzyme or first enzyme composition is added to liquefy at least part of the solids present in the biomass and to keep the viscosity of the cellulose containing biomass below 1000 cP, preferably below 800 cP, more preferably below 600 cP in the liquefaction step; followed by a saccharification step in which a second enzyme composition is added to form oligomeric and/or monomeric sugars; and whereby the first enzyme or first enzyme composition is different from the second enzyme composition; whereby the first enzyme or first enzyme composition comprises an endoglucanase; whereby the second enzyme composition comprises a cellulase; and whereby the first enzyme or first enzyme composition comprises more endogluconase than the second enzyme composition (expressed in protein wt %).