公开(公告)号：US5679320A

公开(公告)日：1997-10-21

申请号：US259569

申请日：1994-06-14

申请人： Tikva Vogel ,  Avigdor Levanon ,  Moshe M. Werber ,  Rachel Guy ,  Amos Panet ,  Jacob Hartman ,  Hadassa Shaked

发明人： Tikva Vogel ,  Avigdor Levanon ,  Moshe M. Werber ,  Rachel Guy ,  Amos Panet ,  Jacob Hartman ,  Hadassa Shaked

IPC分类号： A61K49/00 ,  A61K51/08

CPC分类号： A61K49/0004 ,  A61K49/0002 ,  A61K49/0008 ,  A61K51/082 ,  A61K51/088 ,  A61K2123/00

摘要： This invention provides an imaging agent which comprises a polypeptide labeled with an imageable marker, such polypeptide having an amino acid sequence substantially present in the fibrin binding domain of naturally-occurring human fibronectin and being capable of binding to fibrin. The invention further provides a method wherein the imaging agent is used for imagifig a fibrin-containing substance, i.e., a thrombus or atherosclerotic plaque. Further provided are plasmids for expression of polypeptides having an amino acid sequence substantially present in the fibrin binding domain of naturally-occurring human fibronectin and being capable of binding to fibrin, hosts containing these plasmids, methods of producing the polypeptides, methods of treatment using the polypeptides, and methods of recovering, refolding and reoxidizing the polypeptides. The invention also provides for purified polypeptides substantially free of other substances of human origin which have an amino acid sequence substantially present in the fibrin binding domain of naturally-occurring human fibronectin and which are capable of binding to fibrin.

摘要翻译： 本发明提供一种成像剂，其包含用可成像标记物标记的多肽，所述多肽具有基本上存在于天然存在的人纤连蛋白的纤维蛋白结合结构域中并且能够结合纤维蛋白的氨基酸序列。 本发明还提供了一种方法，其中显像剂用于含血纤维蛋白的物质，即血栓或动脉粥样硬化斑块。 还提供了用于表达多肽的质粒，其具有基本上存在于天然存在的人纤连蛋白的纤维蛋白结合结构域中的氨基酸序列，并且能够结合纤维蛋白，含有这些质粒的宿主，产生多肽的方法，使用 多肽和回收，重折叠和再氧化多肽的方法。 本发明还提供基本上不含人源的其它物质的纯化多肽，其具有基本上存在于天然存在的人纤连蛋白的纤维蛋白结合结构域中并且能够结合纤维蛋白的氨基酸序列。