专利检索 ap:("Koji Ushizawa" OR "Michiko Kawamoto" OR "Shoko Yamamoto" OR "Kumiko Yuki" OR "Yoko Ikeda" OR "Mitsuaki Yamamoto") AND inv:"Koji Ushizawa" 第 3 页

21.

发明授权
Method and reagent for quantitative analysis of 3-oxo-5.beta.-steroid 失效
标题翻译： 3-OXO-5（BETA）-STEROID的定量分析方法和试剂

公开(公告)号：US5085989A

公开(公告)日：1992-02-04

申请号：US574525

申请日：1990-08-28

申请人： Koji Ushizawa , Takae Shigihara , Maki Akemichi , Toshiyuki Akimoto , Senkichi Nagasaki , Miyoshi Hirata

发明人： Koji Ushizawa , Takae Shigihara , Maki Akemichi , Toshiyuki Akimoto , Senkichi Nagasaki , Miyoshi Hirata

IPC分类号： C12Q1/32

CPC分类号： C12Q1/32

摘要： A quantitative analysis of 3-oxo-5.beta.-steroid is carried out by acting 3-oxo-5.beta.-steroid.DELTA..sup.4 -dehydrogenase on a sample in the presence of a reducing chromophoric agent, and measuring the optical density of the chromophoric substance thereby produced. A typical reducing chromophoric agent is a tetrazolium compound. The invention also provides a reagent for the quantitative analysis of 3-oxo-5.beta.-steroid comprising 3-oxo-5.beta.-steroid.DELTA..sup.4 -dehydrogenase and a tetrazolium compound. The analysis and reagent provide a simple and reliable method for a liver function test.