公开(公告)号：US07135341B2

公开(公告)日：2006-11-14

申请号：US11094644

申请日：2005-03-30

申请人： Nery Ortiz ,  Sandra Socarras

发明人： Nery Ortiz ,  Sandra Socarras

IPC分类号： G01N31/00

CPC分类号： G01N33/96 ,  G01N15/1459 ,  G01N2015/1006 ,  G01N2015/1402 ,  G01N2496/05 ,  Y10T436/10 ,  Y10T436/101666 ,  Y10T436/106664 ,  Y10T436/107497 ,  Y10T436/108331 ,  Y10T436/25 ,  Y10T436/25125

摘要： A method of making a reference control containing a nucleated red blood cell component includes providing a blood cell containing a nucleus; treating the blood cell with a treatment solution to alter a nucleus property from a natural value to a target value suitable for simulating nucleated red blood cells on a blood analyzer; and suspending treated blood cell in a suspension medium to form a reference control. The method also includes integrating the nucleated red blood cell component with white blood cell, red blood cell, platelet and reticulocyte components. Further disclosed is a cell treatment composition for altering a nucleus property, which includes a conditioning component, a lytic component for permeating cell membrane, and a fixing component for preserving the cell nucleus. Also disclosed is a method of using the reference control for measurement of nucleated red blood cells on a blood analyzer.

摘要翻译： 制备含有核红细胞成分的参照对照的方法包括提供含有核的血细胞; 用处理溶液处理血细胞以将核性质从天然值改变为适合于在血液分析仪上模拟有核红细胞的目标值; 并将经处理的血细胞悬浮在悬浮介质中以形成参照对照。 该方法还包括将有核红细胞成分与白细胞，红细胞，血小板和网织红细胞成分相结合。 进一步公开了用于改变核性质的细胞处理组合物，其包括调理组分，用于渗透细胞膜的溶解组分和用于保留细胞核的固定组分。 还公开了在血液分析仪上使用用于测量有核红细胞的参考对照的方法。