公开(公告)号：US20200270686A1

公开(公告)日：2020-08-27

申请号：US16089114

申请日：2017-03-28

Applicant: Osaka University

Inventor： Kohji Nishida ,  Atsushi Kumanogoh ,  Noriyasu Hashida

IPC: C12Q1/6883

Abstract: The principal purpose of the present invention is to provide a biomarker that makes it possible to conveniently and accurately assess corneal or conjunctival disease, and can use lacrimal fluid as the sample thereof. In addition, a main object of the present invention is to provide a biomarker that makes it possible to conveniently and accurately evaluate central serous chorioretinopathy. The present invention also provides a diagnostic kit containing a reagent capable of detecting the biomarker, and a diagnosis method that uses the biomarker. It is possible to use mitochondrial DNA included in lacrimal fluid as the biomarker for corneal or conjunctival disease.

公开(公告)号：US20190390274A1

公开(公告)日：2019-12-26

申请号：US16480938

申请日：2018-01-26

Applicant: Osaka University

Inventor： Kohji Nishida ,  Ryuhei Hayashi ,  Toru Okubo

IPC: C12Q1/6881

Abstract: An object of the present invention is to provide a method for determining anatomical sites of a body surface tissue. Provided is a method for determining an anatomical site of origin of a body surface tissue specimen, the method comprising the steps of:(A) selecting at least 8 kinds of genes from the group consisting of a Hox gene family and a Pax6 gene; (B) measuring the expression levels of the genes selected in the above (A) in the body surface tissue specimen; and (C) determining the anatomical site of origin of the body surface tissue specimen based on the expression levels or a combination of the expression levels measured in the above (B).

公开(公告)号：US10457913B2

公开(公告)日：2019-10-29

申请号：US15543021

申请日：2016-01-12

Applicant: Osaka University

Inventor： Kohji Nishida ,  Yuzuru Sasamoto ,  Ryuhei Hayashi

IPC: C12N5/071 ,  C07K14/47 ,  C12N15/86

Abstract: The present invention relates to a method for inducing a keratin 12-positive corneal epithelioid cell from a surface ectoderm-derived cell. More specifically, the present invention relates to a method for inducing a keratin 12-positive corneal epithelioid cell, comprising introducing PAX6, KLF4, and OCT4 into a surface ectoderm-derived cell, such as an oral mucosal epithelial cell, and a corneal epithelioid cell induced by the method.

公开(公告)号：US20180010093A1

公开(公告)日：2018-01-11

申请号：US15542200

申请日：2016-01-13

Applicant: Osaka University

Inventor： Kohji Nishida ,  Ryuhei HAYASHI ,  Yuki ISHIKAWA

IPC: C12N5/079 ,  C12N5/074

CPC classification number: C12N5/0621 ,  C12N5/0607 ,  C12N5/10 ,  C12N2500/90 ,  C12N2501/117 ,  C12N2501/727 ,  C12N2506/45

Abstract: The present invention relates to a method for inducing the differentiation of corneal epithelial cells from pluripotent stem cells. More specifically, the present invention relates to a method for autonomously differentiating pluripotent stem cells, such as human iPS cells, into ectodermal cell lineage in a serum-free medium without using feeder cells and inducing the differentiation of the resultant ocular surface ectodermal lineage cells into corneal epithelial cells.