公开(公告)号：US20050136411A1

公开(公告)日：2005-06-23

申请号：US10744434

申请日：2003-12-22

申请人： Patrick Collins ,  Khanh Nguyen ,  Diane Ilsley

发明人： Patrick Collins ,  Khanh Nguyen ,  Diane Ilsley

IPC分类号： C12Q1/68 ,  C12P19/34 ,  G01N33/48 ,  G01N33/50 ,  G06F19/00

CPC分类号： C12Q1/6809 ,  C12Q1/6865 ,  C12Q2531/101 ,  C12Q2527/125 ,  C12Q2525/143

摘要： Methods and compositions for linearly amplifying mRNA from small RNA samples to produce antisense RNA are provided In the subject methods, mRNA is converted to a transcription template product by using a promoter primer, where the resultant product at least includes a first strand cDNA template domain and a double-stranded RNA polymerase promoter. A feature of the subject methods is that the ratio of the promoter-primer to the input RNA is chosen to provide for a high yield of selective RNA product. Additional optional features include the use of a thermostabilizing agent, e.g., raffinose, trehalose, etc., in the reverse transcription step and/or the use of a polyalkylene oxide, e.g., a PEG, in the in vitro transcription step. Also provided are compositions and kits that find use in practicing the subject methods. The subject invention finds use a variety of different applications in which the preparation of linearly amplified amounts of cRNA is desired.

摘要翻译： 提供从小RNA样品线性扩增mRNA以产生反义RNA的方法和组合物在本主题方法中，通过使用启动子引物将mRNA转化为转录模板产物，其中所得产物至少包含第一链cDNA模板结构域和 双链RNA聚合酶启动子。 本发明方法的特征是选择启动子 - 引物与输入RNA的比例以提供高产率的选择性RNA产物。 另外的任选特征包括在逆转录步骤中使用热稳定剂，例如棉子糖，海藻糖等，和/或在体外转录步骤中使用聚环氧烷，例如PEG。 还提供了用于实践主题方法的组合物和试剂盒。 本发明发现使用其中需要制备线性扩增量的cRNA的多种不同应用。

公开(公告)号：US5040734A

公开(公告)日：1991-08-20

申请号：US492261

申请日：1990-02-28

申请人： Ronald M. Belchamber ,  Patrick Collins

发明人： Ronald M. Belchamber ,  Patrick Collins

IPC分类号： B02C25/00 ,  G01N29/04

CPC分类号： B02C25/00 ,  G01N29/046 ,  G01N2291/02408

摘要： A method for the determination of the value of a property of the material within a mill during the comminution of solid material within the mill comprises the steps of:(a) detecting the noise intensity of milling by means of an analog electrical signal, the intensity of which is proportional to the intensity of the noise in the audio frequency range,(b) converting the analog signal to a digital signal,(c) passing the digital signal through a digital band-pass filter to select at least two frequency bands, and(d) analyzing the data from the bands by means of a multivariate statistical technique to obtain the value of the property. The method is particularly suitable for determining the particle size distribution of material being ground in a ball mill.

摘要翻译： 在磨机中固体材料粉碎期间确定磨机内材料性质值的方法包括以下步骤：（a）通过模拟电信号检测铣削的噪声强度，强度 （b）将模拟信号转换为数字信号，（c）使数字信号通过数字带通滤波器以选择至少两个频带，其中， 和（d）通过多元统计技术分析来自频带的数据以获得该属性的值。 该方法特别适用于确定在球磨机中被研磨的材料的粒度分布。