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1.发明申请Methods and compositions for linear mRNA amplification from small RNA samples 审中-公开来自小RNA样品的线性mRNA扩增的方法和组合物公开(公告)号：US20050136411A1
公开(公告)日：2005-06-23
申请号：US10744434
申请日：2003-12-22
申请人： Patrick Collins , Khanh Nguyen , Diane Ilsley
发明人： Patrick Collins , Khanh Nguyen , Diane Ilsley
CPC分类号： C12Q1/6809 , C12Q1/6865 , C12Q2531/101 , C12Q2527/125 , C12Q2525/143
摘要： Methods and compositions for linearly amplifying mRNA from small RNA samples to produce antisense RNA are provided In the subject methods, mRNA is converted to a transcription template product by using a promoter primer, where the resultant product at least includes a first strand cDNA template domain and a double-stranded RNA polymerase promoter. A feature of the subject methods is that the ratio of the promoter-primer to the input RNA is chosen to provide for a high yield of selective RNA product. Additional optional features include the use of a thermostabilizing agent, e.g., raffinose, trehalose, etc., in the reverse transcription step and/or the use of a polyalkylene oxide, e.g., a PEG, in the in vitro transcription step. Also provided are compositions and kits that find use in practicing the subject methods. The subject invention finds use a variety of different applications in which the preparation of linearly amplified amounts of cRNA is desired.
摘要翻译： 提供从小RNA样品线性扩增mRNA以产生反义RNA的方法和组合物在本主题方法中，通过使用启动子引物将mRNA转化为转录模板产物，其中所得产物至少包含第一链cDNA模板结构域和 双链RNA聚合酶启动子。 本发明方法的特征是选择启动子 - 引物与输入RNA的比例以提供高产率的选择性RNA产物。 另外的任选特征包括在逆转录步骤中使用热稳定剂，例如棉子糖，海藻糖等，和/或在体外转录步骤中使用聚环氧烷，例如PEG。 还提供了用于实践主题方法的组合物和试剂盒。 本发明发现使用其中需要制备线性扩增量的cRNA的多种不同应用。
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公开(公告)号：US08723427B2
公开(公告)日：2014-05-13
申请号：US13439975
申请日：2012-04-05
IPC分类号： H05B37/02
CPC分类号： H05B33/0851 , H05B33/0824
摘要： Board level conditions associated with the operation of multiple LEDs are sensed and used to control a driver that powers the LEDs. The driver is controlled via a 0-10V control interface. The board-level conditions include, but are not limited to, temperature, ambient light, light intensity, operating time, time of day, current, and voltage. An on-board intelligent (OBI) controller processes the 0-10V control signal before it is provided to the driver to better control the LEDs. In some systems the OBI controller works in conjunction with a separate 0-10V controller that controls one or more luminaires.
摘要翻译： 检测与多个LED的操作相关联的电路板电平状况，并用于控制为LED供电的驱动器。 驱动器通过0-10V控制接口进行控制。 板级条件包括但不限于温度，环境光，光强度，工作时间，时间，电流和电压。 车载智能（OBI）控制器在提供给驾驶员以更好地控制LED之前处理0-10V控制信号。 在某些系统中，OBI控制器与控制一个或多个照明灯具的独立0-10V控制器配合使用。
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公开(公告)号：US20130098069A1
公开(公告)日：2013-04-25
申请号：US13278428
申请日：2011-10-21
申请人： Patrick Collins
发明人： Patrick Collins
IPC分类号： F25D5/00
CPC分类号： F25D5/02
摘要： A self-cooling beverage container comprising a space between outer and inner walls, the space containing a liquid that is mixed with a chemical contained within a compartment within the base of the beverage container. The compartment within the base of the beverage container has a rupture portion located within the space between the outer and inner walls, and pressure on the base of the beverage container causes the rupture portion to open, allowing mixing of the chemical and the liquid. The chemical and the liquid react in an endothermic reaction, cooling the contents of the beverage container.
摘要翻译： 一种自冷饮料容器，其包括在外壁和内壁之间的空间，所述空间包含与容纳在饮料容器的基部内的隔室内的化学品混合的液体。 饮料容器基部内的隔室具有位于外壁和内壁之间的空间内的破裂部分，并且饮料容器底部的压力导致破裂部分打开，允许化学品和液体混合。 化学品和液体在吸热反应中反应，冷却饮料容器的内容物。
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公开(公告)号：US20120063850A1
公开(公告)日：2012-03-15
申请号：US13229920
申请日：2011-09-12
申请人： Patrick Collins
发明人： Patrick Collins
CPC分类号： E02B17/02 , E02B17/027 , E02B2017/0039 , E02B2017/0043 , E02B2017/0047 , E02B2017/0091 , F03D13/10 , F05B2240/95 , Y02E10/727
摘要： A method of installing marine equipment, the method including providing a marine apparatus comprising the marine equipment and a buoyancy device including at least a first and a second tank. The first tank containing air and the second tank containing a buoyant liquid having a specific gravity of less than 0.8 g/cm3. The method including providing a template on the seabed and guiding the marine apparatus down onto the template. Also a buoyancy device comprising at least a first and a second tank, the first tank containing a first fluid and the second tank containing a second fluid.
摘要翻译： 一种安装船用设备的方法，所述方法包括提供包括所述船用设备的船用设备和至少包括第一和第二箱的浮力设备。 含有空气的第一罐和第二罐含有比重小于0.8g / cm 3的浮力液体。 该方法包括在海床上提供模板并将船舶装置引导到模板上。 还有一种浮力装置，包括至少第一和第二罐，第一罐包含第一流体，第二罐包含第二流体。
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公开(公告)号：US20070070209A1
公开(公告)日：2007-03-29
申请号：US11516249
申请日：2005-10-28
IPC分类号： H04N5/232
CPC分类号： H04N7/181 , H04N21/21805 , H04N21/25435 , H04N21/2668 , H04N21/2747 , H04N21/4728 , H04N21/6125 , H04N21/6587
摘要： An apparatus for viewing an event at an event site includes a plurality of cameras disposed about an event site, the cameras providing views of the event site from at least two different camera angles, and a viewer in communication with the plurality of cameras, the viewer enabling a user to selectively view the event by means of at least two of the plurality of cameras.
摘要翻译： 用于在事件现场观看事件的装置包括围绕事件站点设置的多个摄像机，所述摄像机从至少两个不同的摄像机角度提供事件站点的视图，以及与多个摄像机通信的观看者，观看者 使得用户能够通过所述多个相机中的至少两个来选择性地查看所述事件。
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6.发明申请公开(公告)号：US20050282174A1
公开(公告)日：2005-12-22
申请号：US10871303
申请日：2004-06-19
摘要： Methods and systems for identifying and selecting nucleic acid probes for detecting a target with a nucleic acid probe array or microarray, comprising selecting a plurality of candidate probes, forming a plurality of clusters from the plurality of candidate probes according to hybridization characteristics of the candidate probes, forming at least one SuperCluster from the clusters; and selecting at least one probe from each SuperCluster for the probe array.
摘要翻译： 用于鉴定和选择用于用核酸探针阵列或微阵列检测靶的核酸探针的方法和系统，包括根据候选探针的杂交特征选择多个候选探针，从多个候选探针形成多个簇 ，从簇中形成至少一个超级簇; 并从每个SuperCluster中为探针阵列选择至少一个探针。
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7.发明申请Method and system for quantifying and removing spatial-intensity trends in microarray data 有权用于量化和消除微阵列数据中的空间强度趋势的方法和系统公开(公告)号：US20050273268A1
公开(公告)日：2005-12-08
申请号：US10859868
申请日：2004-06-02
申请人： Jayati Ghosh , Bill Peck , Eric Leproust , Charles Troup , Glenda Delenstarr , Patrick Collins , John Corson , Paul Wolber , Xiangyang Zhou
发明人： Jayati Ghosh , Bill Peck , Eric Leproust , Charles Troup , Glenda Delenstarr , Patrick Collins , John Corson , Paul Wolber , Xiangyang Zhou
摘要： A method and system for quantifying and correcting spatial-intensity trends for each channel of a microarray data set having one or more channels. The method and system of one embodiment of the present invention selects a set of features from each channel of the microarray data set. Based on the selected set of features, a surface is used to determine the intensities for all features in each channel of the microarray data set. Spatial-intensity trends within the microarray data set are quantified, based on the surface to the intensities for each channel of the microarray data set. After the surface has been determined, the spatial-intensity trend can be removed from the microarray data set.
摘要翻译： 一种用于量化和校正具有一个或多个通道的微阵列数据集的每个通道的空间强度趋势的方法和系统。 本发明的一个实施例的方法和系统从微阵列数据集的每个通道中选择一组特征。 基于所选择的特征集合，使用表面来确定微阵列数据集的每个通道中的所有特征的强度。 基于微阵列数据集的每个通道的表面到强度，量化微阵列数据集内的空间强度趋势。 在确定表面之后，可以从微阵列数据集中去除空间强度趋势。
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公开(公告)号：US08784011B2
公开(公告)日：2014-07-22
申请号：US13229920
申请日：2011-09-12
申请人： Patrick Collins
发明人： Patrick Collins
CPC分类号： E02B17/02 , E02B17/027 , E02B2017/0039 , E02B2017/0043 , E02B2017/0047 , E02B2017/0091 , F03D13/10 , F05B2240/95 , Y02E10/727
摘要： A method of installing marine equipment, the method including providing a marine apparatus comprising the marine equipment and a buoyancy device including at least a first and a second tank. The first tank containing air and the second tank containing a buoyant liquid having a specific gravity of less than 0.8 g/cm3. The method including providing a template on the seabed and guiding the marine apparatus down onto the template. Also a buoyancy device comprising at least a first and a second tank, the first tank containing a first fluid and the second tank containing a second fluid.
摘要翻译： 一种安装船用设备的方法，所述方法包括提供包括所述船用设备的船用设备和至少包括第一和第二箱的浮力设备。 含有空气的第一罐和第二罐含有比重小于0.8g / cm 3的浮力液体。 该方法包括在海床上提供模板并将船舶装置引导到模板上。 还有一种浮力装置，包括至少第一和第二罐，第一罐包含第一流体，第二罐包含第二流体。
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公开(公告)号：US20070070210A1
公开(公告)日：2007-03-29
申请号：US11516250
申请日：2005-10-28
CPC分类号： H04N7/181 , H04N5/222 , H04N5/247 , H04N5/28 , H04N21/21805 , H04N21/25435 , H04N21/2668 , H04N21/2747 , H04N21/4728 , H04N21/6125 , H04N21/6587
摘要： A method of viewing an event at an event site includes the steps of: providing an apparatus for viewing the event at the event site, the apparatus including a plurality of cameras disposed about the event site, the cameras providing views of the event site from at least two different camera angles, and a viewer in communication with the plurality of cameras, the viewer enabling a user to selectively view the event by means of at least two of the plurality of cameras; and viewing the event using the viewer.
摘要翻译： 在事件现场观看事件的方法包括以下步骤：提供用于在事件现场观看事件的装置，该装置包括围绕事件现场布置的多个照相机，照相机提供事件现场的视图， 至少两个不同的相机角度，以及与所述多个相机通信的观看者，所述观看者使得用户能够通过所述多个照相机中的至少两个来选择性地观看所述事件; 并使用查看器查看事件。
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公开(公告)号：US20060147273A1
公开(公告)日：2006-07-06
申请号：US10559712
申请日：2004-06-03
申请人： Patrick Collins
发明人： Patrick Collins
摘要： A method of insulating pipeline bundles used in recovering hydrocarbons from wells is disclosed. A polymeric substance, such as an orthphosphate ester, is injected into the annulus between a carrier pipe and the hydrocarbon conveying tubulars. A ferric salt, such as ferric sulphate, may be added as a gelling agent. The mixture results in a gel having a dynamic viscosity of greater than (1000) PaS. The gel insulates the inner hydrocarbon-conveying tubulars from the surrounding seawater thus helping to maintain the relatively high temperature therewithin. This in turn reduces the likelihood for chemicals, such as hydrates, to be precipitated out of the oil phase.
摘要翻译： 公开了一种用于从井中回收碳氢化合物的绝缘管道束的方法。 将聚合物质如磷酸酯酯注入载体管和烃输送管之间的环中。 可以加入铁盐如硫酸铁，作为胶凝剂。 混合物产生具有大于（1000）PaS的动态粘度的凝胶。 凝胶将内部烃输送管与周围的海水隔离，从而有助于保持其中较高的温度。 这又降低了化学物质（例如水合物）从油相中沉淀出来的可能性。
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