公开(公告)号：US6100447A

公开(公告)日：2000-08-08

申请号：US022586

申请日：1998-02-12

申请人： Liying Wu ,  Raymond L. Rodriguez

发明人： Liying Wu ,  Raymond L. Rodriguez

IPC分类号： C12N15/82 ,  A01H1/00 ,  A01H5/00 ,  C12N15/87 ,  A01H4/100

CPC分类号： C12N15/8207 ,  C12N15/8205

摘要： A method for stably transforming barley from mature barley seeds as starting material is disclosed. The method involves germinating mature barley seeds until early shoot development occurs, exposing scutellar or embryo tissue cells on the embryo side of germinated seeds, and introducing foreign DNA into the cells. The cells are initially grown under conditions that allow expression of a selectable marker introduced with the foreign DNA, then on a callus-growth medium effective to suppress callus formation in the absence of the selectable marker. Successfully transformed calli can be cultured in suspension to obtain a desired foreign protein, or regenerated into plants, to obtain the foreign protein from the transformed plants, e.g., germinated seeds.

摘要翻译： 公开了从成熟大麦种子稳定转化大麦作为起始原料的方法。 该方法包括发芽成熟的大麦种子，直到发生早期的芽发育，在发芽种子的胚胎侧暴露出黄芩或胚胎组织细胞，并将外源DNA引入细胞。 细胞最初在允许表达外源DNA引入的选择性标记的条件下生长，然后在不存在可选择标记的情况下在有效抑制愈伤组织形成的愈伤组织生长培养基上生长。 成功转化的愈伤组织可以在悬浮液中培养以获得期望的外源蛋白质，或再生成植物，从转化的植物例如发芽种子获得外源蛋白质。

公开(公告)号：US6066781A

公开(公告)日：2000-05-23

申请号：US23173

申请日：1998-02-13

申请人： Thomas D. Sutliff ,  Raymond L. Rodriguez

发明人： Thomas D. Sutliff ,  Raymond L. Rodriguez

IPC分类号： C07K14/81 ,  C12N15/29 ,  C12N15/82 ,  A01H4/00 ,  C12N5/04

CPC分类号： C12N15/8221 ,  C07K14/8125 ,  C12N15/8234 ,  C12N15/8235 ,  C12N15/8242 ,  C12N15/8257

摘要： A chimeric gene for use in producing a mature protein in secreted form by stably transformed plant cells is disclosed. The gene includes a DNA coding sequence encoding a fusion protein having an (i) N-terminal moiety corresponding to the portion of the rice .alpha.-amylase signal sequence peptide identified by SEQ ID: 1 and, (ii) immediately adjacent the C-terminal amino acid of said portion, a protein moiety corresponding to the protein to be produced. Also disclosed are a fusion protein encoded by the gene, and a method of producing a mature protein in secreted form by plant cells.

摘要翻译： 公开了一种用于通过稳定转化的植物细胞产生分泌形式的成熟蛋白质的嵌合基因。 该基因包括编码融合蛋白的DNA编码序列，其具有对应于由SEQ ID：1鉴定的水稻α-淀粉酶信号序列肽的部分的（i）N末端部分，和（ii）紧邻C末端 所述部分的氨基酸，对应于待产生蛋白质的蛋白质部分。 还公开了由该基因编码的融合蛋白质，以及由植物细胞分泌形成的成熟蛋白质的方法。

公开(公告)号：US5994628A

公开(公告)日：1999-11-30

申请号：US460507

申请日：1995-06-02

申请人： Raymond L. Rodriguez

发明人： Raymond L. Rodriguez

IPC分类号： A01H5/00 ,  C12N5/10 ,  C12N15/09 ,  C12N15/29 ,  C12N15/82 ,  C12P21/02 ,  C12N15/12

CPC分类号： C12N15/8222 ,  C12N15/8234 ,  C12N15/8242 ,  C12N15/8257

摘要： This invention provides for the secretion of heterologous protein in plant systems. In particular, this invention provides for the production of heterologous proteins by malting of monocot plant seeds. The heterologous genes are expressed during germination of the seeds and isolated from a malt. Also disclosed are chimeric genes, vectors and methods relating to the present invention. Protein production by cell culture techniques is also described.