公开(公告)号：US20090220520A1

公开(公告)日：2009-09-03

申请号：US11914752

申请日：2006-05-24

申请人： Villoo Morawala Patell

发明人： Villoo Morawala Patell

IPC分类号： A61K39/395 ,  C07K16/28 ,  C12P21/04

CPC分类号： C07K16/2893 ,  C07K2317/24

摘要： The present invention relates to the recombinant method used for the production of soluble form monoclonal antibody that binds to CD52. The procedure describes the de novo synthesis of the nucleic acid sequence encoding anti-CD 52, transformation of the constructed nucleic acid sequences into competent bacteria and the sub-cloning of the same into mammalian expression vectors for expression of the desired protein. DNA constructs comprising the control elements associated with the gene of interest has been disclosed. The nucleic acid sequence of interest has been codon optimized to permit expression in the suitable mammalian host cells.

摘要翻译： 本发明涉及用于生产结合CD52的可溶形式单克隆抗体的重组方法。 该方法描述了编码抗-CD52的核酸序列的从头合成，将构建的核酸序列转化为感受态细菌，并将其亚克隆至用于表达所需蛋白质的哺乳动物表达载体中。 已经公开了包含与感兴趣的基因相关的对照元件的DNA构建体。 感兴趣的核酸序列已被密码子优化以允许在合适的哺乳动物宿主细胞中表达。