公开(公告)号：US20060078552A1

公开(公告)日：2006-04-13

申请号：US10507736

申请日：2003-03-14

申请人： Sylvain Arnould ,  Patrick Chames ,  Andre Choulika ,  Jean-Charles Epinat ,  Emmanuel Lacroix

发明人： Sylvain Arnould ,  Patrick Chames ,  Andre Choulika ,  Jean-Charles Epinat ,  Emmanuel Lacroix

IPC分类号： C12Q1/70 ,  A61K38/46 ,  C12Q1/68 ,  C07H21/04 ,  C12N9/22

CPC分类号： C12N9/22 ,  C07K2319/00

摘要： This patent application relates to hybrid and/or single-chain rare-cutting endonucleases, called meganucleases, which recognized and cleave a specific nucleotide sequence, to polynucleotide sequences encoding for said rare-cutting endonucleases, to a vector comprising one of said polynucleotide sequences, to a cell or animal non-human comprising one of said polynucleotide sequences or said rare-cutting endonucleases, to a process for producing one of said rare-cutting endonucleases and any use of the disclosed products and methods. More particularly, this invention contemplates any use of such rare-cutting endonuclease for genetic engineering and gene therapy.

摘要翻译： 该专利申请涉及称为大范围核酸酶的杂交和/或单链稀释内切核酸酶，其将编码所述稀少切割内切核酸酶的多核苷酸序列识别并切割特定核苷酸序列，所述多核苷酸序列包含所述多核苷酸序列之一， 涉及包含所述多核苷酸序列之一或所述稀少切割内切核酸酶的细胞或动物非人，涉及产生所述稀有切割内切核酸酶之一的方法和所公开的产品和方法的任何用途。 更具体地，本发明考虑了这种稀释内切核酸酶用于遗传工程和基因治疗的任何用途。