PROCESS FOR DIFFERENTIATION OF VASCULAR ENDOTHELIAL PROGENITOR CELLS FROM EMBRYOID BODIES DERIVED FROM EMBRYONIC STEM CELLS USING HYPOXIC MEDIA CONDITION
    32.
    发明申请
    PROCESS FOR DIFFERENTIATION OF VASCULAR ENDOTHELIAL PROGENITOR CELLS FROM EMBRYOID BODIES DERIVED FROM EMBRYONIC STEM CELLS USING HYPOXIC MEDIA CONDITION 审中-公开
    使用HYPOXIC MEDIA CONDITION从胚胎干细胞衍生的EMBRYOID体中分离血管内皮祖细胞的方法

    公开(公告)号:US20100221833A1

    公开(公告)日:2010-09-02

    申请号:US12733661

    申请日:2008-08-14

    IPC分类号: C12N5/0735

    摘要: The present invention provides a process for differentiation of vascular endothelial progenitor cells from embryoid bodies derived from embryonic stem cells, the process comprising: (a) treating a culture medium comprising embryoid bodies derived from embryonic stem cells such that the concentration of oxygen dissolved in the culture medium is in the range of about 1 ppm to about 5 ppm; (b) culturing the culture medium prepared in step (a) in an incubator in which the oxygen (O2) tension is equal to or less than about 15% to differentiate the embryoid bodies into vascular endothelial progenitor cells; and (c) isolating the vascular endothelial progenitor cells from the culture medium obtained in step (b).

    摘要翻译: 本发明提供从源自胚胎干细胞的胚状体分化血管内皮祖细胞的方法,该方法包括:(a)处理包含来源于胚胎干细胞的胚状体的培养基,使得溶于 培养基在约1ppm至约5ppm的范围内; (b)在氧(O 2)张力等于或小于约15%的培养箱中培养步骤(a)中制备的培养基,以将胚状体分化成血管内皮祖细胞; 和(c)从步骤(b)中获得的培养基中分离血管内皮祖细胞。

    Anti-doming compositions for a shadow-mask and processes for preparing the same
    34.
    发明授权
    Anti-doming compositions for a shadow-mask and processes for preparing the same 失效
    用于荫罩的防穹顶组合物及其制备方法

    公开(公告)号:US06342756B1

    公开(公告)日:2002-01-29

    申请号:US09504127

    申请日:2000-02-15

    IPC分类号: H01J2980

    摘要: An anti-doming composition including a mixture of electron reflecting material such as bismuth oxide, and a zeolite, and a shadow-mask coated with the composition restrict temperature increase by preventing heat transfer, an anti-doming composition including lead and a ZnO, B2O3, Bi2O3 or a mixture thereof, and a shadow-mask coated with the composition protect mechanical expansion of a shadow-mask, and an anti-doming composition including ferroelectrics such as PZT, PT, PZ or PLZT, and a shadow-mask coated with the composition restrict temperature increase by changing the energy of electron beams into non-thermal energy. The shadow-mask of the present invention reduces about 30 to 50% of the anti-doming ratio and has a low production cost and easiness during processes.

    摘要翻译: 包含氧化铋等电子反射材料和沸石的混合物的反拱顶组合物和通过防止热传导而涂覆有该组合物而限制温度升高的荫罩,包含铅和ZnO的抗拱顶组合物B 2 O 3 ,Bi2O3或它们的混合物,以及涂有组合物的荫罩防止荫罩的机械膨胀,以及包含铁电体如PZT,PT,PZ或PLZT的抗拱顶组合物和涂有 该组合物通过将电子束的能量改变为非热能来限制温度升高。 本发明的阴罩减少了约30-50%的抗拱起率,并且在生产成本和加工过程中容易度低。

    Process for isolating vascular endothelial cells from embryoid bodies differentiated from embryonc stem cells
    35.
    发明授权
    Process for isolating vascular endothelial cells from embryoid bodies differentiated from embryonc stem cells 有权
    从胚胎干细胞分化的胚状体分离血管内皮细胞的方法

    公开(公告)号:US07919318B2

    公开(公告)日:2011-04-05

    申请号:US11913295

    申请日:2007-06-13

    IPC分类号: C12N5/02

    摘要: The present invention provides a process for isolating vascular endothelial cells from embryoid bodies differentiated from embryonic stem cells, which comprises: (a) treating embryoid bodies differentiated from embryonic stem cells with 0.005-0.015% trypsin and 0.05-0.15 mM ethylenediaminetetraacetate (EDTA) to obtain vascular endothelial cell clusters; and (b) treating the vascular endothelial cell clusters with 0.1-0.5% trypsin and 0.5-2 mM EDTA so as to separate the vascular endothelial cell clusters into single cells.

    摘要翻译: 本发明提供了从胚胎干细胞分化的胚状体分离血管内皮细胞的方法,其包括:(a)用0.005-0.015%胰蛋白酶和0.05-0.15mM乙二胺四乙酸(EDTA)将胚胎干细胞分化为胚胎干细胞, 获得血管内皮细胞簇; 和(b)用0.1-0.5%胰蛋白酶和0.5-2mM EDTA处理血管内皮细胞簇,以将血管内皮细胞簇分离成单个细胞。

    Anti-doming composition for a shadow-mask and processes for preparing
the same
    36.
    发明授权
    Anti-doming composition for a shadow-mask and processes for preparing the same 失效
    用于阴影掩模的反拱形组合物及其制备方法

    公开(公告)号:US6094003A

    公开(公告)日:2000-07-25

    申请号:US955164

    申请日:1997-10-21

    IPC分类号: H01J9/14 H01J29/07 H01J31/20

    摘要: An anti-doming composition including a mixture of electron reflecting material such as bismuth oxide, and a zeolite, and a shadow-mask coated with the composition restrict temperature increase by preventing heat transfer, an anti-doming composition including lead and a ZnO, B.sub.2 O.sub.3, Bi.sub.2 O.sub.3 or a mixture thereof, and a shadow-mask coated with the composition protect mechanical expansion of a shadow-mask, and an anti-doming composition including ferroelectrics such as PZT, PT, PZ or PLZT, and a shadow-mask coated with the composition restrict temperature increase by changing the energy of electron beams into non-thermal energy. The shadow-mask of the present invention reduces about 30 to 50% of the anti-doming ratio and has a low production cost and easiness during processes.

    摘要翻译: 包含氧化铋等电子反射材料和沸石的混合物的反拱顶组合物和通过防止热传导而涂覆有该组合物而限制温度升高的荫罩,包含铅和ZnO的抗拱顶组合物B 2 O 3 ,Bi2O3或它们的混合物,以及涂有组合物的荫罩防止荫罩的机械膨胀,以及包含铁电体如PZT,PT,PZ或PLZT的抗拱顶组合物和涂有 该组合物通过将电子束的能量改变为非热能来限制温度升高。 本发明的阴罩减少了约30-50%的抗拱起率,并且在生产成本和加工过程中容易度低。