公开(公告)号：US06198024B1

公开(公告)日：2001-03-06

申请号：US09067800

申请日：1998-04-28

申请人： Martin F. Yanofsky ,  Cristina Ferrandiz

发明人： Martin F. Yanofsky ,  Cristina Ferrandiz

IPC分类号： C12N1582

CPC分类号： C07K14/415 ,  C12N15/8222 ,  C12N15/8234 ,  C12N15/8261 ,  C12N15/8266 ,  Y02A40/146

摘要： The present invention provides a non-naturally occurring seed plant that is characterized by delayed seed dispersal due to ectopic expression of a nucleic acid molecule encoding an AGL8-like gene product. Further provided herein is a non-naturally occurring seed plant, such as an agl1 agl5 double mutant, that is characterized by delayed seed dispersal due to suppression of AGL1 and AGL5 expression in the seed plant. The invention also provides a substantially purified dehiscence zone-selective regulatory element, which includes a nucleotide sequence that confers selective expression upon an operatively linked nucleic acid molecule in the valve margin or dehiscence zone of a seed plant. Also provided by the invention are kits for producing a transgenic seed plant characterized by delayed seed dispersal, such kits containing a dehiscence zone-selective regulatory element.

摘要翻译： 本发明提供一种非天然存在的种子植物，其特征在于由于编码AGL8样基因产物的核酸分子的异位表达而延迟的种子分散。 本文进一步提供的是非天然存在的种子植物，例如agl1 agl5双突变体，其特征在于由于抑制种子植物中AGL1和AGL5表达而延迟种子扩散。 本发明还提供了基本上纯化的开裂带选择性调节元件，其包括赋予种子植物的瓣膜边缘或开裂带中的可操作连接的核酸分子上的选择性表达的核苷酸序列。 本发明还提供了用于生产以延迟种子分散为特征的转基因种子植物的试剂盒，该试剂盒含有开裂带选择性调节元件。

公开(公告)号：US5811536A

公开(公告)日：1998-09-22

申请号：US592214

申请日：1996-01-26

申请人： Martin F. Yanofsky

发明人： Martin F. Yanofsky

IPC分类号： C07K14/415 ,  C12N15/29 ,  C12N15/82 ,  C12Q1/68 ,  C12N5/04

CPC分类号： C12Q1/6895 ,  C07K14/415 ,  C12N15/827 ,  C12Q2600/13 ,  C12Q2600/156

摘要： The present invention provides a nucleic acid molecule encoding a CAULIFLOWER (CAL) gene product such as a nucleic acid molecule encoding Arabidopsis thaliana CAL and a nucleic acid molecule encoding Brassica oleracea CAL (BoCAL). The invention also provides a nucleic acid molecule encoding a truncated CAL gene product such as a nucleic acid molecule encoding Brassica oleracea var. botrytis CAL (BobCAL). The invention also provides a nucleic acid containing the Arabidopsis thaliana CAL gene, a nucleic acid molecule containing the Brassica oleracea CAL gene and a nucleic acid molecule containing the Brassica oleracea var. botrytis CAL gene. The invention further provides a kit for converting shoot meristem to floral meristem and a kit for promoting early flowering in an angiosperm. The invention provides a CAL polypeptide and an antibody that specifically binds CAL polypeptide. In addition, the invention provides the truncated BobCAL polypeptide and an antibody that specifically binds truncated BobCAL polypeptide. The invention further provides a method of identifying a Brassica having a modified CAL allele by detecting a polymorphism associated with a CAL locus, where the CAL locus comprises a modified CAL allele that does not encode an active CAL gene product.

公开(公告)号：US5744693A

公开(公告)日：1998-04-28

申请号：US460512

申请日：1995-06-02

申请人： Elliot M. Meyerowitz ,  Martin F. Yanofsky ,  Hong Ma

发明人： Elliot M. Meyerowitz ,  Martin F. Yanofsky ,  Hong Ma

IPC分类号： C07K14/415 ,  C12N15/82 ,  A01H4/00 ,  C12N5/14

CPC分类号： C07K14/415 ,  C12N15/827 ,  C12N15/8289

摘要： The invention includes plants having at least one cell transformed with a vector comprising at least a portion of an agamous nucleic acid. Such plants have a phenotype characterized by altered floral development such as an AG or AP2 phenotype. The invention also includes vectors comprising at least a portion of an agamous nucleic acid operably linked to a promoter other than the promoter naturally associated with the agamous nucleic acid. In an alternate embodiment, the vector comprises at least a portion of an agamous nucleic acid operably linked in an antisense orientation to a promoter. The invention also includes methods using such vectors for producing plants having altered floral development.

摘要翻译： 本发明包括具有至少一个细胞转化的植物，所述细胞用包含抗体核酸的至少一部分的载体转化。 这样的植物具有以改变的花卉发育为特征的表型，例如AG或AP2表型。 本发明还包括含有至少一部分可操作地连接到除与天然与核酸相关的启动子之外的启动子的抗体核酸的载体的载体。 在替代实施方案中，载体包含以反义方向可操作地连接至启动子的抗体核酸的至少一部分。 本发明还包括使用这种载体生产具有改变的花卉发育的植物的方法。