公开(公告)号：US20160185828A1

公开(公告)日：2016-06-30

申请号：US14786304

申请日：2014-04-23

Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE

Inventor： Neel Satish Joshi ,  Peter Quoc Nguyen ,  Zsofia Botyanszki

IPC: C07K14/245 ,  C07K14/21

CPC classification number: C07K14/245 ,  C07K14/21 ,  C07K2319/20 ,  C07K2319/735

Abstract: Described herein are methods and compositions relating to engineered curii fibers, e.g. CsgA polypeptide. In some embodiments, the methods and compositions described herein relate to functionalized biofilms. In one aspect, described herein is an engineered CsgA polypeptide, comprising a CsgA polypeptide with a C-terminal display tag flanking the CsgA polypeptide; wherein the display tag comprises an activity polypeptide and a linker sequence. In some embodiments, the display tag and/or the activity polypeptide comprises a polypeptide selected from the group consisting of metal binding domain (MBD); Spy Tag; graphene binding (GBP); carbon nanotube binding (CBP); gold binding (A3); CT43; FLAG; Z8; E14; QBP1; CLP12; and AFP8.

Abstract translation: 本文描述了与工程化纤维纤维有关的方法和组合，例如 CsgA多肽。 在一些实施方案中，本文所述的方法和组合物涉及官能化生物膜。 在一个方面，本文描述的是工程化的CsgA多肽，其包含具有位于CsgA多肽侧翼的C末端显示标签的CsgA多肽; 其中所述显示标签包含活性多肽和接头序列。 在一些实施方案中，显示标签和/或活性多肽包含选自金属结合结构域（MBD）的多肽; 间谍标签 石墨烯结合（GBP）; 碳纳米管结合（CBP）; 金装订（A3）; CT43; 旗; Z8; E14; QBP1; CLP12; 和AFP8。