公开(公告)号：US20240301088A1

公开(公告)日：2024-09-12

申请号：US18515458

申请日：2023-11-21

Applicant: AUTOLUS LIMITED

Inventor： Martin Pulé ,  John Anderson ,  Simon Thomas

IPC: C07K16/30 ,  A61K35/17 ,  A61K38/17 ,  A61K39/00 ,  A61K39/395 ,  C07K14/705 ,  C07K14/725 ,  C12N15/85

CPC classification number: C07K16/3084 ,  A61K35/17 ,  A61K38/1774 ,  A61K38/179 ,  A61K39/39558 ,  C07K14/7051 ,  C07K14/70517 ,  C07K14/70521 ,  C07K14/70578 ,  C12N15/85 ,  A61K2039/505 ,  C07K2317/21 ,  C07K2317/24 ,  C07K2317/52 ,  C07K2317/53 ,  C07K2317/565 ,  C07K2317/622 ,  C07K2317/73 ,  C07K2319/00 ,  C07K2319/03 ,  C07K2319/20 ,  C07K2319/30 ,  C12N2510/00

Abstract: Provision of a chimeric antigen receptor (CAR) comprising a disialoganglioside (GD2)-binding domain which comprises⋅a) a heavy chain variable region (VH) having complementarity determining regions (CDRs) with the following sequences:⋅b) a light chain variable region (VL) having CDRs with the following sequences: T cells expressing such a CAR are useful in the treatment of some cancers.

公开(公告)号：US12054708B2

公开(公告)日：2024-08-06

申请号：US16345895

申请日：2017-10-30

Applicant: Universität Zürich

Inventor： Markus Seeger ,  Pascal Egloff ,  Iwan Zimmermann

IPC: C12N15/10 ,  C07K16/12 ,  G01N33/68

CPC classification number: C12N15/1065 ,  C07K16/12 ,  C12N15/1034 ,  C12N15/1093 ,  G01N33/6845 ,  G01N33/6848 ,  C07K2317/22 ,  C07K2317/569 ,  C07K2319/20 ,  C07K2319/21 ,  C07K2319/22 ,  C07K2319/23 ,  C07K2319/24 ,  C07K2319/41 ,  C07K2319/43 ,  C12Q2563/119 ,  C12Q2563/185 ,  C12N15/1034 ,  C12Q2563/119 ,  C12Q2563/185 ,  C12N15/1065 ,  C12Q2563/119 ,  C12Q2563/185

Abstract: The invention relates to a method for identifying and quantifying a polypeptide from a library of polypeptides. The method comprises the steps of: 1—providing a polypeptide library and a detection tag library, 2—generating a nested library comprising the polypeptides and the detection tags, 3—sequencing the nested library, 4—selecting a member of the nested library in one or several selection steps that are independent of a physical genotype-phenotype linkage, 5—isolating the detection tag from the selected polypeptide, 6—identifying and quantifying the detection tag by mass spectrometry, 7—obtaining the sequence of the selected polypeptide. The invention also relates to a collection of polypeptides, a collection of detection tags, and a collection of plasmid vectors.

公开(公告)号：US20240209046A1

公开(公告)日：2024-06-27

申请号：US18453221

申请日：2023-08-21

Applicant: Korea Institute of Ceramic Engineering and Technology ,  ToolBio. Co

Inventor： Sung Hyun Kim ,  Jin Ho Bang ,  Mi Hyun Choi ,  Seho Park

IPC: C07K14/47 ,  C07K1/30 ,  C07K14/475 ,  C07K14/485 ,  C07K14/50 ,  C07K14/51 ,  C07K14/605 ,  C07K14/745 ,  C07K14/78 ,  C12N9/08 ,  C12N9/50 ,  C12N15/70 ,  C12P21/02

CPC classification number: C07K14/4728 ,  C07K1/303 ,  C07K14/475 ,  C07K14/485 ,  C07K14/50 ,  C07K14/51 ,  C07K14/605 ,  C07K14/745 ,  C07K14/78 ,  C12N9/0065 ,  C12N9/506 ,  C12N15/70 ,  C12P21/02 ,  C12Y111/01007 ,  C12Y304/22044 ,  C07K2319/20

Abstract: The present disclosure relates to a method for expressing, water-solubilizing, and purifying protein by using a calsequestrin-tag (CSQ-tag). Also provided are: a recombinant expression vector containing a CSQ-tag and a target protein; a host cell transformed using the recombinant expression vector; and a method for expressing and purifying a target protein by using a CSQ tag.

公开(公告)号：US11993652B2

公开(公告)日：2024-05-28

申请号：US17329115

申请日：2021-05-24

Applicant: Fred Hutchinson Cancer Center

Inventor： Stanley R. Riddell ,  Lingfeng Liu

IPC: A61K38/17 ,  A61K35/17 ,  A61K35/545 ,  A61K38/20 ,  C07K14/705 ,  C07K14/725 ,  C07K16/28 ,  C07K19/00 ,  C12N5/0783 ,  G01N33/569 ,  A61K35/12 ,  A61K39/00

CPC classification number: C07K16/2803 ,  A61K35/17 ,  A61K35/545 ,  A61K38/1774 ,  A61K38/2013 ,  A61K38/2086 ,  C07K14/705 ,  C07K14/7051 ,  C07K14/70521 ,  C07K14/70578 ,  C07K16/2818 ,  C07K16/2878 ,  C12N5/0637 ,  C12N5/0638 ,  G01N33/56972 ,  A61K2035/124 ,  A61K2039/505 ,  A61K2039/507 ,  C07K2317/622 ,  C07K2317/64 ,  C07K2319/03 ,  C07K2319/20 ,  C07K2319/22 ,  C07K2319/33 ,  C07K2319/95 ,  C12N2501/2302 ,  C12N2510/00 ,  A61K38/2013 ,  A61K2300/00 ,  A61K38/2086 ,  A61K2300/00

Abstract: The present disclosure relates to tagged chimeric effector molecules and receptor molecules thereof for genetically engineering a host cell, wherein the recombinant host cell can be identified, isolated, sorted, induced to proliferate, tracked or eliminated. For example, a T cell may be recombinantly modified for use in adoptive immunotherapy.

公开(公告)号：US20240168032A1

公开(公告)日：2024-05-23

申请号：US18340009

申请日：2023-06-22

Applicant: Duke University

Inventor： Homme W. Hellinga ,  Malin J. Allert

IPC: G01N33/66 ,  C07K14/245 ,  C12Q1/54

CPC classification number: G01N33/66 ,  C07K14/245 ,  C12Q1/54 ,  C07K2319/20 ,  G01N2333/195 ,  G01N2333/245

Abstract: The present subject matter provides glucose biosensors as well as compositions, devices, and methods comprising such biosensors.

公开(公告)号：US11951186B2

公开(公告)日：2024-04-09

申请号：US17078843

申请日：2020-10-23

Applicant: Cercacor Laboratories, Inc.

Inventor： Venkatramanan Krishnamani ,  Sergei Petrovich Balashov ,  Kevin Hughes Pauley ,  Ruiqi Long ,  Hung The Vo

IPC: A61B5/1455 ,  A61B5/1459 ,  A61B5/1473 ,  A61B5/1477 ,  A61K49/00 ,  C07K14/245 ,  C07K14/435 ,  A61B5/145

CPC classification number: A61K49/0056 ,  A61B5/1455 ,  A61B5/1459 ,  A61B5/14735 ,  A61B5/1477 ,  A61K49/0045 ,  C07K14/245 ,  C07K14/43595 ,  A61B5/14532 ,  C07K2319/20 ,  C07K2319/60

Abstract: Some embodiments disclosed herein pertain to indicator compounds used to detect the presence of and/or an amount of an analyte. In some embodiments, the indicator compounds are fusion proteins. In some embodiments, when the analyte binds to the indicator compound, the indicator compound undergoes a conformational change. In some embodiments, the conformational change results in a luminescent signal that allows quantification of the amount of analyte present.

公开(公告)号：US20240018501A1

公开(公告)日：2024-01-18

申请号：US18070554

申请日：2022-11-29

Applicant: The Board of Trustees of the University of Illinois

Inventor： Wilfred A. van der Donk ,  Weixin Tang

IPC: C12N9/52 ,  C07K14/195 ,  C07K14/315 ,  C07K14/32 ,  C12P21/06 ,  C12P21/02 ,  C07K14/31 ,  C12N15/62

CPC classification number: C12N9/52 ,  C07K14/195 ,  C07K14/315 ,  C07K14/32 ,  C12P21/06 ,  C12P21/02 ,  C07K14/31 ,  C12N15/62 ,  C12Y304/21 ,  C07K2319/50 ,  C07K2319/20 ,  C07K2319/01 ,  C07K2319/40 ,  C07K2319/70 ,  C07K2319/21 ,  C07K2319/24

Abstract: An isolated nucleic acid that includes an open reading frame encoding a lanthipeptide protease polypeptide for scarless tag removal from a polypeptide is presented. Reagents, expression constructs and methods are also provided for preparing a scarless tag polypeptide product from a tagged polypeptide precursor containing a lanthipeptide protease cleavage site. The reagents are directed to novel lanthipeptide proteases and expression constructs and polypeptide precursors that include highly specific lanthipeptide protease substrate recognition sequence. Methods are provided that enable scarless tag removal from a cognate lanthipeptide, a non-cognate lanthipeptide or a heterologous polypeptide that includes extraneous amino acid sequences, such as leader peptides and tags.

公开(公告)号：US11873504B2

公开(公告)日：2024-01-16

申请号：US16335516

申请日：2017-09-28

Applicant: The Regents of the University of California

Inventor： Jennifer A Doudna ,  Jillian F. Banfield ,  David Burstein ,  Lucas Benjamin Harrington ,  Steven C. Strutt

IPC: C12N15/85 ,  C12N9/22 ,  C12N15/113 ,  C12N15/10 ,  C12N15/86 ,  C12N15/90 ,  C07K19/00 ,  C12N9/10 ,  C12N15/52 ,  C12N15/62 ,  C12N15/74 ,  C12N15/88

CPC classification number: C12N15/8509 ,  C07K19/00 ,  C12N9/1007 ,  C12N9/1025 ,  C12N9/22 ,  C12N15/102 ,  C12N15/113 ,  C12N15/52 ,  C12N15/62 ,  C12N15/74 ,  C12N15/85 ,  C12N15/86 ,  C12N15/88 ,  C12N15/902 ,  C07K2319/02 ,  C07K2319/03 ,  C07K2319/06 ,  C07K2319/09 ,  C07K2319/20 ,  C12N2310/20 ,  C12N2740/13043 ,  C12N2740/16043 ,  C12N2750/14143

Abstract: The present disclosure provides CasX proteins, nucleic acids encoding the CasX proteins, and modified host cells comprising the CasX proteins and/or nucleic acids encoding same. CasX proteins are useful in a variety of applications, which are provided. The present disclosure provides CasX guide RNAs that bind to and provide sequence specificity to the CasX proteins, nucleic acids encoding the CasX guide RNAs, and modified host cells comprising the CasX guide RNAs and/or nucleic acids encoding same. CasX guide RNAs are useful in a variety of applications, which are provided. The present disclosure provides archaeal Cas9 polypeptides and nucleic acids encoding same, as well as their associated archaeal Cas9 guide RNAs and nucleic acids encoding same.

公开(公告)号：US20230408515A1

公开(公告)日：2023-12-21

申请号：US18035809

申请日：2021-11-10

Applicant: Massachusetts Institute of Technology

Inventor： Hadley Sikes Johnson ,  Eric Alexander Miller ,  Seunghyeon Kim

IPC: G01N33/569 ,  G01N33/58 ,  C07K14/005

CPC classification number: G01N33/56983 ,  G01N33/581 ,  C07K14/005 ,  G01N2333/165 ,  G01N2469/20 ,  G01N2400/26 ,  C12N2770/20022 ,  C07K2319/20

Abstract: The present disclosure relates to proteins comprising a target-binding domain for detection of anti-SARS-CoV-2 antibodies, methods, compositions and kits thereof.

公开(公告)号：US20230288408A1

公开(公告)日：2023-09-14

申请号：US18015906

申请日：2021-07-15

Applicant: Technische Universität Müncher ,  Karlsruher Institut für Technologie

Inventor： Sonja Berensmeier ,  Sebastian Schwaminger ,  Silvia Blank-shim ,  Paula Fraga García ,  Yasmin Kaveh-Baghbaderani ,  Alexander Zanker ,  Stefan Rauwolf ,  Wolfgang Wenzel

IPC: G01N33/543 ,  C07K7/06

CPC classification number: G01N33/54326 ,  C07K7/06 ,  C07K2319/20

Abstract: The present invention relates to a peptide consisting of a sequence of 5 or 6 to 30, preferably 6 to 12, most preferably 10 to 12 amino acids, wherein (a) at least ⅓, preferably at least ½ of said amino acids are amino acids having a functional group or a side chain which is positively charged at neutral pH; and (b) at least two amino acids, preferably at least three are histidines.