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    Fluorescent labeling complexes with large stokes shift formed by coupling together cyanine and other flourochromes capable of resonance energy transfer
    31.
    发明申请
    Fluorescent labeling complexes with large stokes shift formed by coupling together cyanine and other flourochromes capable of resonance energy transfer 有权
    通过将能够共振能量转移的花青和其它荧光色素耦合在一起而形成具有大的斯托克斯位移的荧光标记复合物

    公开(公告)号：US20030220502A1

    公开(公告)日：2003-11-27

    申请号：US10300459

    申请日：2002-11-20

    发明人： Alan S. Waggoner ,  Swati R. Mujumdar ,  Ratnakar B. Mujumdar

    IPC分类号： C07F005/02 ,  C07D419/02 ,  C07D417/02 ,  C07D413/02 ,  C07D43/02

    CPC分类号： G01N33/582 ,  C09B11/22 ,  C09B23/06 ,  C09B23/083 ,  C09B23/086 ,  C09B23/10 ,  G01N33/533 ,  Y10S436/80

    摘要： The present invention provides low molecular weight fluorescent labeling complexes with large wavelength shifts between absorption of one dye in the complex and emission from another dye in the complex. These complexes can be used, for example, for multiparameter fluorescence cell analysis using a single excitation wavelength. The low molecular weight of the complex permits materials labeled with the complex to penetrate cell structures for use as probes. The labeling complexes are synthesized by covalently attaching through linkers at least one cyanine fluorochrome to another low molecular weight fluorochrome to form energy donor-acceptor complexes. Resonance energy transfer from an excited donor to fluorescent acceptor provides wavelength shifts up to 300 nm. The fluorescent labeling complexes preferably contain reactive groups for the labeling of functional groups on target compounds, such as derivatized oxy and deoxy polynucleic acids, antibodies, enzymes, proteins and other materials. The complexes may also contain functional groups permitting covalent reaction with materials containing reactive groups.

    摘要翻译： 本发明提供了在复合物中的一种染料的吸收和复合物中另一种染料的发射之间具有大的波长偏移的低分子量荧光标记复合物。 这些复合物可用于例如使用单个激发波长的多参数荧光细胞分析。 复合物的低分子量允许用复合物标记的材料穿透细胞结构用作探针。 通过将至少一个花青荧光染料共价连接到另一个低分子量荧光染料上以形成能量供体 - 受体复合物来合成标记复合物。 从受激供体到荧光受体的共振能量转移提供高达300nm的波长漂移。 荧光标记复合物优选含有用于标记靶化合物上的官能团的反应性基团，例如衍生的氧基和脱氧多核酸，抗体，酶，蛋白质和其它材料。 络合物还可以含有允许与含有反应性基团的材料共价反应的官能团。

    Triaryl-methane dyestuffs
    34.
    发明授权
    Triaryl-methane dyestuffs 失效
    三芳基甲烷染料

    公开(公告)号：US4115413A

    公开(公告)日：1978-09-19

    申请号：US878952

    申请日：1969-11-21

    申请人： Roderich Raue ,  Willi Eifler ,  Hans-Peter Kuhlthau

    发明人： Roderich Raue ,  Willi Eifler ,  Hans-Peter Kuhlthau

    IPC分类号： C09B11/22 ,  C07C93/14

    CPC分类号： C09B11/22

    摘要： Triaryl-methane dyestuffs which are free from sulfonic acid and carboxylic acid groups and are selected from the group consisting of dyestuffs having one of the following four formulae: ##STR1## wherein R is alkyl, aralkyl, cycloalkyl or aryl; R.sub.1 is hydrogen, alkyl, aralkyl or cycloalkyl; R.sub.2 is alkyl, aralkyl, cycloalkyl or aryl; R.sub.1 and R.sub.2 additionally including divalent alkylene when joined together with the nitrogen or with a carbon atom in the o-position in the ring A to form a heterocyclic ring; R.sub.3 is hydrogen, alkyl, aralkyl, aryl, aralkoxy, aryloxy, halogen, carboxylic acid ester radical, carbonamido, sulfonamido, cyano, nitro, alkylsulfonyl, aryalkylsulfonyl, arylsulfonyl or acyl; and X is the radical of an anion; ##STR2## wherein R is alkyl or cycloalkyl; R.sub.1 is hydrogen, alkyl, aralkyl, cycloalkyl or aryl; R.sub.2 is alkyl, cycloalkyl, aralkyl or aryl, halogen or cyano; R.sub.1 and R.sub.2 additionally include divalent alkylene when joined together with the nitrogen atom or with a carbon atom in the o-position in the ring A to form a heterocyclic ring; R.sub.3 is hydrogen or alkyl; R.sub.4 is alkyl or cycloalkyl; and X is the radical of an anion; ##STR3## wherein R is alkyl or cycloalkyl; R.sub.1 is hydrogen or methyl; R.sub.2 is methyl, aryl, or cycloalkyl; R.sub.3 is hydrogen or alkyl; R.sub.4 is alkyl or cycloalkyl; R.sub.3 and R.sub.4 additionally include divalent alkylene when joined together to form a carbocyclic ring; and X is the radical of an anion; and ##STR4## wherein at least one R is halogen, alkyl, or alkoxy and the other R's are hydrogen, halogen, alkyl, or alkoxy; R.sub.1 is hydrogen or methyl; R.sub.2 is methyl, aryl, or cycloalkyl; and X is the radical of an anion.Process of preparing the above dyestuffs by condensation of an appropriate benzophenone with an aromatic amine or by the oxidation of the corresponding leuco compound. These dyestuffs are particularly useful in dyeing or printing materials made of polymers of acrylonitrile, polymers of as.-dicyanoethylene, and acid-modified aromatic polyesters.

    摘要翻译： 不含磺酸和羧酸基团的三芳基甲烷染料，选自具有以下四种式之一的染料：

    Oscar bally
    40.
    发明授权
    Oscar bally 失效

    公开(公告)号：US524235A

    公开(公告)日：1894-08-07

    申请号：US524235D

    CPC分类号： C09B11/22