公开(公告)号：US20140088317A1

公开(公告)日：2014-03-27

申请号：US14091496

申请日：2013-11-27

Applicant: Virginia Polytechnic Institute and State University

Inventor： Zhiyou Wen

IPC: C11C1/00

CPC classification number: C11C1/002 ,  C12N1/12 ,  C12N1/32 ,  C12P7/6427 ,  C12P7/6472

Abstract: The present invention relates to various methods to produce a variety of omega-3 fatty acids from various species of alga using crude glycerol as a substrate for algal growth. In one embodiment, the present invention relates to various methods to produced docosahexaenoic acid (DHA) from a Schizochytrium, Phaeodactylum, Thraustochytrid, Ulkenia, and/or Labyrinthulea species of alga. In another embodiment, the present invention relates to various methods to produced eicosapentaenoic acid (EPA) from a Schizochytrium, Phaeodactylum, Thraustochytrid, Ulkenia, and/or Labyrinthulea species of alga. In one instance, the methods of the present invention utilize crude glycerol as at least a portion of the culture medium for the various micro-organisms disclosed herein to enable the production of one or more omega-3 fatty acids. In one embodiment, the crude glycerol of the present invention can be generated from a biodiesel process as a substrate for the production of either docosahexaenoic acid (DHA) or eicosapentaenoic acid (EPA).

Abstract translation: 本发明涉及使用粗甘油作为藻类生长的底物从各种藻类生产各种ω-3脂肪酸的各种方法。 在一个实施方案中，本发明涉及从裂殖酵母，Phaeodactylum，Thraustochytrid，Ulkenia和/或藻类Labyrinthulea物种生产二十二碳六烯酸（DHA）的各种方法。 在另一个实施方案中，本发明涉及从裂殖酵母，Phaeodactylum，Thraustochytrid，Ulkenia和/或藻类Labyrinthulea物种生产二十碳五烯酸（EPA）的各种方法。 在一个实例中，本发明的方法利用粗甘油作为本文公开的各种微生物的培养基的至少一部分，以使得能够生产一种或多种ω-3脂肪酸。 在一个实施方案中，本发明的粗甘油可以从作为生产二十二碳六烯酸（DHA）或二十碳五烯酸（EPA））的底物的生物柴油方法产生。