公开(公告)号：US06665072B2

公开(公告)日：2003-12-16

申请号：US09793853

申请日：2001-02-26

申请人： Clifford C. Hoyt

发明人： Clifford C. Hoyt

IPC分类号： G01N2125

CPC分类号： G01N21/6445 ,  G01N21/6428 ,  G01N21/6452 ,  G01N2021/6421 ,  G01N2021/6441 ,  G01N2021/6471

摘要： An apparatus for performing fluorescence detection of two or more biochemical probes and/or fluorescence measurement of fluorescence intensity at two or more spectral bands of light emitted from at least one sample spot is disclosed. The apparatus simultaneously directs emitted fluorescent light from multiple probes and/or at multiple spectral bands to different spots on a single pixelated detector.

摘要翻译： 公开了一种用于在从至少一个采样点发射的光的两个或更多个光谱带处进行两个或更多个生物化学探针的荧光检测和/或荧光测量的荧光测量的装置。 该装置同时将来自多个探针和/或多个光谱带的发出的荧光指向单个像素化检测器上的不同点。

公开(公告)号：US5953087A

公开(公告)日：1999-09-14

申请号：US838625

申请日：1997-04-11

申请人： Clifford C. Hoyt

发明人： Clifford C. Hoyt

IPC分类号： G02F1/13 ,  G02F1/1333

CPC分类号： G02F1/133308 ,  G02F2001/133311

摘要： A liquid crystal cell is described which includes adaptive means to relieve hydrostatic pressure and means for restraining the aperture portion of the cell, and which may be assembled together with thick members such as precision windows, or may be built into optical assemblies incorporating several cells placed optically in series, such as tunable filters or multiple-cell optical switches, without defeating the pressure-relief mechanism.

摘要翻译： 描述了一种液晶单元，其包括用于缓解静水压力的自适应装置和用于限制电池的开口部分的装置，并且其可以与诸如精密窗口的厚构件组装在一起，或者可以内置在并入几个电池的光学组件中 光学上串联的，例如可调谐滤波器或多单元光学开关，而不会破坏减压机构。

公开(公告)号：US5943129A

公开(公告)日：1999-08-24

申请号：US906665

申请日：1997-08-07

申请人： Clifford C. Hoyt ,  Peter J. Miller

发明人： Clifford C. Hoyt ,  Peter J. Miller

IPC分类号： G01N21/64

CPC分类号： G01N21/6456 ,  G01N21/6445

摘要： A fluorescence imaging system for epi-illumination wherein the usual dichroic beamsplitter is replaced by a polarizing beamsplitter (PBS). The sample is excited with light that is linearly polarized to a significant degree, and fluorescent emission light is collected in the orthogonal linear polarization state. Excitation light that is scattered or reflected by the sample is rejected by the PBS, while the desired emission light is captured for imaging by a detector. By eliminating the usual dichroic beamsplitter member, the imaging system removes the barriers to multi-spectral imaging that such members conventionally impose. A wavelength-selective birefringent network may also be interposed between the beamsplitter and the sample for converting the polarization of either the excitation or the emission light to the orthogonal state without defeating this desirable rejection property, thus permitting measurement of the sample emissions in either or both linear polarization states for assessing fluorescence polarization.

摘要翻译： 用于外照明的荧光成像系统，其中通常的二向色分束器被偏振分束器（PBS）代替。 用明显线性极化的光激发样品，并以正交的线偏振状态收集荧光发射光。 被样品散射或反射的激发光被PBS拒绝，同时捕获所需的发射光以通过检测器进行成像。 通过消除通常的二向色分束器部件，成像系统消除了这些部件通常施加的多光谱成像的障碍。 波长选择性双折射网络也可以插在分束器和样品之间，用于将激发或发射光的极化转换为正交状态，而不会消除该期望的抑制特性，从而允许在任一或两者中测量样品发射 用于评估荧光偏振的线性极化状态。