公开(公告)号：US12117398B2

公开(公告)日：2024-10-15

申请号：US17923792

申请日：2021-05-04

申请人： ETH Zurich

发明人： David J. Norris ,  Daniel Gisler ,  Carin Rae Lightner

IPC分类号： G01N21/64

CPC分类号： G01N21/6445

摘要： A system (1) for measuring the optical activity of a sample (2) comprises at least one frequency modulation device (3), at least one synchronization device (4), and at least one detection device (5). The frequency modulation device (3) is configured to modulate a frequency of incident electromagnetic radiation being emitted from a sample (2) and/or being irradiated on to a sample (2) with at least one frequency modulation signal (Sf). The synchronization device (4) is configured to receive the at least one frequency modulation signal (Sf) and to emit at least one detection modulation signal (Sd) being synchronized with the at least one frequency modulation signal (Sf). The system (1) is configured such that the detection device (5) detects the electromagnetic radiation (EMs) in synchronization with the detection modulation signal (Sd).

公开(公告)号：US20240053269A1

公开(公告)日：2024-02-15

申请号：US17766192

申请日：2020-10-06

申请人： AQUILA BIOLABS GMBH

发明人： Konrad Herzog ,  David Frank

IPC分类号： G01N21/64 ,  G01N21/94

CPC分类号： G01N21/6486 ,  G01N21/6445 ,  G01N21/94 ,  G01N2021/6441 ,  G01N2021/646

摘要： Methods and devices for evaluating viability and/or degree of contamination of cells cultured in a reactor, which includes providing luminescent dye in the reactor whose luminescence anisotropy is influenced by the viability or the degree of contamination of the cells, exciting the luminescent dye to luminesce by irradiating polarized light into the medium. polarizing light emitted by the luminescent dye that leaves the medium, and detecting the polarized emitted light with a light sensor, where the polarized emitted light is detected as at least two luminescence values in at least two different polarization angles, and determining at least one anisotropy value from the at least two luminescence values by means of mathematical methods, which correlates with the viability or the degree of contamination of the cells.

公开(公告)号：US11761896B1

公开(公告)日：2023-09-19

申请号：US18077421

申请日：2022-12-08

申请人： PALO ALTO RESEARCH CENTER INCORPORATED

发明人： Qiushu Chen ,  Peter Kiesel ,  Jacob N. Chamoun ,  Norman Nan Shi ,  Noble M. Johnson

IPC分类号： G01N21/64

CPC分类号： G01N21/645 ,  G01N21/6445 ,  G01N21/6486 ,  G01N2021/6463

摘要： An analyzing system includes a detection area with a transparent window past which an analyte moves and emits or transmits light. One or more polarizing elements receive and polarize the light into respective two or more different polarization components. One or more optical detectors receive the respective two or more polarization components and generate respective at least two signals in response. A processor is coupled to the optical detectors and configured to determine a polarization status of the light from the analyte based on the at least two signals.

公开(公告)号：US20190187162A1

公开(公告)日：2019-06-20

申请号：US16223096

申请日：2018-12-17

申请人： Evanostics LLC

发明人： Ashutosh SHASTRY ,  David PIEHLER ,  Hardeep SANGHERA ,  Michael GLUZCZACK ,  Pranav CHOPRA ,  Yun-Pei CHANG ,  Ameya KANTAK

IPC分类号： G01N33/94 ,  G01N33/487 ,  G01N21/64

CPC分类号： G01N33/94 ,  G01N21/6445 ,  G01N33/487 ,  G01N2021/6439 ,  G01N2201/0221 ,  G01N2201/0612

摘要： Analyte collection and testing systems and methods, and more particularly to disposable oral fluid collection and testing systems and methods. Described herein are methods and apparatuses to achieve significant improvements in the detection of fluorescence signals in the reader.

公开(公告)号：US20180355350A1

公开(公告)日：2018-12-13

申请号：US15996222

申请日：2018-06-01

申请人： Raindance Technologies, Inc.

发明人： Darren Roy Link ,  Laurent Boitard ,  Jeffrey Branciforte ,  Yves Charles ,  Gilbert Feke ,  John Q. Lu ,  David Marran ,  Ahmadali Tabatabai ,  Michael Weiner ,  Wolfgang Hinz ,  Jonathan M. Rothberg

IPC分类号： C12N15/10 ,  G01N33/68 ,  B01F13/00 ,  G01N33/543 ,  G01N33/536 ,  C40B70/00 ,  C40B60/12 ,  C40B60/08 ,  C40B50/08 ,  C40B40/04 ,  C40B30/04 ,  C12Q1/6876 ,  C12Q1/6874 ,  C12Q1/686 ,  C07K1/04 ,  B82Y30/00 ,  B82Y5/00 ,  B01L3/00 ,  B01J19/00 ,  G01N33/542 ,  G01N21/64 ,  C12Q1/6869 ,  C12Q1/6834 ,  C12Q1/6825 ,  C12Q1/6816 ,  C12Q1/6804 ,  B01L7/00 ,  B01F3/08

CPC分类号： C12N15/1075 ,  B01F3/0807 ,  B01F13/0071 ,  B01F13/0076 ,  B01J19/0046 ,  B01J2219/00286 ,  B01J2219/00459 ,  B01J2219/00466 ,  B01J2219/00468 ,  B01J2219/00479 ,  B01J2219/005 ,  B01J2219/00576 ,  B01J2219/00599 ,  B01J2219/00702 ,  B01J2219/0072 ,  B01J2219/00743 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502746 ,  B01L3/502761 ,  B01L3/502784 ,  B01L3/502792 ,  B01L3/565 ,  B01L7/52 ,  B01L2200/027 ,  B01L2200/0621 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2200/0673 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0896 ,  B01L2400/0415 ,  B01L2400/0424 ,  B01L2400/0487 ,  B01L2400/084 ,  B82Y5/00 ,  B82Y30/00 ,  C07K1/047 ,  C12Q1/6804 ,  C12Q1/6816 ,  C12Q1/6825 ,  C12Q1/6834 ,  C12Q1/686 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q2525/161 ,  C12Q2561/119 ,  C12Q2563/103 ,  C12Q2563/107 ,  C12Q2563/159 ,  C12Q2565/537 ,  C12Q2565/629 ,  C12Q2600/16 ,  C40B30/04 ,  C40B40/04 ,  C40B50/08 ,  C40B60/08 ,  C40B60/12 ,  C40B70/00 ,  G01N21/6428 ,  G01N21/6445 ,  G01N21/6458 ,  G01N33/536 ,  G01N33/542 ,  G01N33/543 ,  G01N33/6845 ,  G01N33/6854 ,  G01N2500/10

摘要： The present invention provides novel microfluidic devices and methods that are useful for performing high-throughput screening assays and combinatorial chemistry. The invention provides for aqueous based emulsions containing uniquely labeled cells, enzymes, nucleic acids, etc., wherein the emulsions further comprise primers, labels, probes, and other reactants. An oil based carrier-fluid envelopes the emulsion library on a microfluidic device, such that a continuous channel provides for flow of the immiscible fluids, to accomplish pooling, coalescing, mixing, sorting, detection, etc., of the emulsion library.

公开(公告)号：US10018608B2

公开(公告)日：2018-07-10

申请号：US14634591

申请日：2015-02-27

申请人： Vitaly Talyansky ,  Edward Talyansky ,  Jose Gasque

发明人： Vitaly Talyansky ,  Edward Talyansky ,  Jose Gasque

IPC分类号： G01N21/64 ,  G01N33/14 ,  G01N21/84

CPC分类号： G01N33/14 ,  G01N21/6428 ,  G01N21/6445 ,  G01N2021/6419 ,  G01N2021/6441 ,  G01N2021/8416

摘要： A system to authenticate and identify items is described herein. An example apparatus includes an illuminating source to illuminate a sample with a first emission of light having a peak wavelength at a first wavelength and a second emission of light having a peak wavelength at a second wavelength, a photo element to detect a first optical response emitted by the sample after the first emission of light and to detect a second optical response emitted by the sample after the second emission of light, and an identifier to identify the sample based on the first optical response and the second optical response.

公开(公告)号：US10004401B2

公开(公告)日：2018-06-26

申请号：US15288840

申请日：2016-10-07

申请人： Ivana Ledesma

发明人： Ralph Zuckerman

IPC分类号： G01N1/00 ,  A61B5/00 ,  A61B5/145 ,  A61B5/1455 ,  G01N21/64

CPC分类号： A61B5/0071 ,  A61B5/14546 ,  A61B5/1455 ,  A61B5/14555 ,  A61B5/14556 ,  A61B5/4842 ,  A61B5/7246 ,  A61B5/7278 ,  G01N21/6445 ,  G01N21/645 ,  G01N2021/6484 ,  G01N2201/0683 ,  G01N2201/12

摘要： A non-invasive measurement of biological tissue reveals information about the function of that tissue. Polarized light is directed onto the tissue, stimulating the emission of fluorescence, due to one or more endogenous fluorophors in the tissue. Fluorescence anisotropy is then calculated. Such measurements of fluorescence anisotropy are then used to assess the functional status of the tissue, and to identify the existence and severity of disease states. Such assessment can be made by comparing a fluorescence anisotropy profile with a known profile of a control.

公开(公告)号：US09874737B2

公开(公告)日：2018-01-23

申请号：US14890243

申请日：2014-05-07

申请人： Forschungszentrum Mikroskopie (FZM), LuciaOptics gemeinnützige UG

发明人： Gerrit Best ,  Christoph Cremer ,  Sabrina Rossberger ,  Stefan Dithmar

IPC分类号： G02B21/36 ,  G01N21/64 ,  G02B21/00 ,  G02B21/16 ,  G02B27/58

CPC分类号： G02B21/367 ,  G01N21/6445 ,  G01N21/6458 ,  G01N2201/0675 ,  G02B21/0076 ,  G02B21/16 ,  G02B21/361 ,  G02B27/58

摘要： A fluorescence microscope for obtaining super-resolution images of a sample labeled with at least one fluorescent label by combining localization microscopy and structured illumination microscopy is provided. The fluorescence microscope includes one or more light sources, an illumination system having a structured illumination path for illuminating the sample with structured illumination light and a localization illumination path for illuminating the sample with localization illumination light.

公开(公告)号：US20170329122A1

公开(公告)日：2017-11-16

申请号：US15666842

申请日：2017-08-02

申请人： NIKON CORPORATION

发明人： Hisao OSAWA

IPC分类号： G02B21/36 ,  G02B21/06 ,  G02B27/58 ,  G01N21/64 ,  G02B21/16

CPC分类号： G02B21/365 ,  G01N21/6428 ,  G01N21/6445 ,  G01N21/6458 ,  G01N2021/6439 ,  G01N2021/6478 ,  G01N2201/0612 ,  G01N2201/062 ,  G01N2201/0635 ,  G02B21/06 ,  G02B21/10 ,  G02B21/125 ,  G02B21/16 ,  G02B21/367 ,  G02B27/58

摘要： A structured illumination microscope includes: a first illumination optical system configured to irradiate, from a first direction, a sample with activating light for activating a fluorescent substance included in the sample; a second illumination optical system configured to irradiate, from a second direction that is different from the first direction, the sample with interference fringes of exciting light for exciting the fluorescent substance; a control unit configured to control a direction and a phase of the interference fringes; an imaging optical system configured to form an image of the sample irradiated with the interference fringes; an imaging element configured to take the image formed by the imaging optical system to generate a first image; and a demodulation unit configured to generate a second image by using a plurality of the first images generated by the imaging element.

公开(公告)号：US20170322155A1

公开(公告)日：2017-11-09

申请号：US15496011

申请日：2017-04-25

申请人： OSRAM GmbH

发明人： Jan Oliver Drumm ,  Jens Richter

IPC分类号： G01N21/64 ,  B60Q11/00 ,  F21V9/16 ,  F21S8/10 ,  F21Y2115/30

CPC分类号： G01N21/6445 ,  B60Q11/005 ,  F21S41/14 ,  F21S41/16 ,  F21S41/295 ,  F21V9/30 ,  F21Y2115/30

摘要： In various embodiments, a lighting device is provided. The lighting device includes a phosphor volume for at least partial wavelength conversion of primary light into secondary light, a primary light semiconductor light source for irradiating the phosphor volume with primary light, a measurement light generating arrangement for generating measurement light having a spectral composition outside the primary light and the secondary light, a measurement light detector sensitive to the measurement light, and a measurement light filter, which is fixedly connected to the phosphor volume and is optically arranged between the measurement light generating arrangement and the measurement light detector.