公开(公告)号：US06166183A

公开(公告)日：2000-12-26

申请号：US957719

申请日：1997-10-27

申请人： Rika Ishikawa ,  Yuji Okada ,  Makoto Kakitani

发明人： Rika Ishikawa ,  Yuji Okada ,  Makoto Kakitani

IPC分类号： C07K14/535

CPC分类号： C07K14/535

摘要： The present invention provides a chemically-modified protein prepared by binding polyethylene glycol to a polypeptide characterized by being the product of expression by a host cell of an exogenous DNA sequence and substantially having the following amino acid sequence: (Het)n - Thr Pro Leu Gly Pro Ala Ser Ser Leu Pro Gln - Ser Phe Leu Leu Lys Cys Leu Glu Gln Val Arg - Lys Ile Gln Gly Asp Gly Ala Ala Leu Gln Glu - Lys Leu Cys Ala Thr Tyr Lys Leu Cys His Pro - Glu Glu Leu Val Leu Leu Gly His Ser Leu Gly - Ile Pro Trp Ala Pro Leu Ser Ser Cys Pro Ser - Gln Ala Leu Gln Leu Ala Cly Cys Leu Ser Gln - Leu His Ser Gly Leu Phe Leu Tyr Gln GIY Leu - Leu Gln Ala Leu Glu Gly Ile Ser Pro Glu Leu - Gly Pro Thr Leu Asp Thr Leu Gln Leu Asp Val - Ala Asp Phe Ala Thr Tbr Ile Trp Gln Gln Het - Glu Glu Leu Gly Het Ala Pro Ala Leu Gln Pro - Thr Gln Gly Ala Het Pro Ala Phe Ala Ser Ala - Phe Gln Arg Arg Ala Gly Gly Val Leu Val Ala - Ser His Leu Gln Ser Phe Leu Glu Val Scr Tyr - Arg Val Leu Arg His Leu Ala Gln Pro (n = 0 or 1) The chemically-modified protein according to the present invention has a neutrophils-increasing activity much more lasted than that of the intact human G-CSF, enabling fewer numbers of administration with a lower dose.

摘要翻译： 本发明提供了通过将聚乙二醇与多肽结合而制备的化学修饰的蛋白质，其特征在于宿主细胞表达外源DNA序列并且基本上具有以下氨基酸序列的产物： - （Het）n - Thr Pro Leu Gly Pro Ala Ser Ser Leu Pro Gln - Ser Phe Leu Leu Lys Cys Leu Glu Gln Val Arg - - Lys Ile Gln Gly Asp Gly Ala Ala Leu Gln Glu - - Lys Leu Cys Ala Thr Tyr Lys Leu Cys His Pro - - Glu Glu Leu Val Leu Leu Gly His Ser Leu Gly - - Ile Pro Trp Ala Pro Leu Ser Ser Cys Pro Ser - - Gln Ala Leu Gln Leu Ala Cly Cys Leu Ser Gln - - Leu His Ser Gly Leu Phe Leu Tyr Gln GIY Leu - - Leu Gln Ala Leu Glu Gly Ile Ser Pro Glu Leu - - Gly Pro Thr Leu Asp Thr Leu Gln Leu Asp Val - - Ala Asp Phe Ala Thr Tbr Ile Trp Gln Gln Het - Glu Glu Leu Gly Het Ala Pro Ala Leu Gln Pro - - Thr Gln Gly Ala Het Pro Ala Phe Ala Ser Ala - - Phe Gln Arg Arg Ala Gly Gly Val Leu Val Ala - - Ser His Leu Gln Ser Phe Leu Glu Val Scr Ty r - Arg Val Leu Arg His Leu Ala Gln Pro - （n = 0或1） - 根据本发明的化学修饰的蛋白具有比完整的人G-CSF的中性粒细胞增加活性更多的持续时间， 使用更少剂量的较少数量的给药。

公开(公告)号：US4778913A

公开(公告)日：1988-10-18

申请号：US930395

申请日：1986-11-14

申请人： Yuji Okada ,  Kenji Kato ,  Motoyuki Sugiura

发明人： Yuji Okada ,  Kenji Kato ,  Motoyuki Sugiura

IPC分类号： C08F4/36 ,  C07C67/00 ,  C07C407/00 ,  C07C409/32 ,  C07C409/34 ,  C07C179/14

CPC分类号： C07C409/32

摘要： A novel peroxydicarbonate containing a non-conjugate type unsaturated bond, represented by the general formula: ##STR1## (wherein R.sub.1 stands for a hydrogen atom or a methyl group and n for an integer of the value of 1 or 2) is particularly useful as a polymerization initiator.

摘要翻译： 含有非结合型不饱和键的新型过氧化二碳酸酯特别适用于下述通式：其中R1表示氢原子或甲基，n表示1或2的整数） 聚合引发剂。

公开(公告)号：US4665132A

公开(公告)日：1987-05-12

申请号：US871913

申请日：1986-06-09

申请人： Yasuo Moriya ,  Nobuyoshi Suzuki ,  Yuji Okada

发明人： Yasuo Moriya ,  Nobuyoshi Suzuki ,  Yuji Okada

IPC分类号： C08F257/02 ,  C08F293/00

CPC分类号： C08F257/02 ,  C08F293/00

摘要： A block copolymer consisting of segments of copolymer A, which is obtained by copolymerizing an aromatic vinyl monomer with a radically copolymerizable peroxycarbonate and has peroxy groups in its main polymer chain, and segments of (co)polymer B, which is different from copolymer A in the monomer composition and does not contain peroxy groups, can modify matrix resin without deteriorating the mechanical property of the resin.

摘要翻译： 由芳族乙烯基单体与可自由基共聚的过氧碳酸酯共聚并在其主聚合物链中具有过氧基团的共聚物A的链段和不同于共聚物A的（共）聚合物B的链段组成的嵌段共聚物， 单体组成并且不含有过氧基团，可以改性基体树脂而不降低树脂的机械性能。