公开(公告)号：US20090325312A1

公开(公告)日：2009-12-31

申请号：US12227320

申请日：2007-05-11

申请人： Ailan Guo ,  Ting-Lei Gu ,  Peter Hornbeck ,  Jeffrey Mitchell ,  Yu Li

发明人： Ailan Guo ,  Ting-Lei Gu ,  Peter Hornbeck ,  Jeffrey Mitchell ,  Yu Li

IPC分类号： G01N33/566 ,  C07K16/18

CPC分类号： G01N33/6842 ,  C07K16/44

摘要： The invention discloses 426 novel acetylation sites identified in signal transduction proteins and pathways underlying human protein acetylation signaling pathways, and provides acetylation-site specific antibodies and heavy-isotope labeled peptides (AQUA peptides) for the selective detection and quantification of these acetylated sites/proteins, as well as methods of using the reagents for such purpose. Among the acetylation sites identified are sites occurring in the following protein types: Methyltransferases, Transcription factors, Transcription coactivators, Translation initiation complex proteins, Oxireductases, Protein kinases, RNA binding proteins, Secreted proteins, Transferases, Transporter proteins, Ubiquitin conjugating system proteins, Motor proteins, Phosphotases, Proteases, Phospholipases, Receptor proteins and Vesicle proteins.

摘要翻译： 本发明公开了在信号转导蛋白中鉴定的426个新的乙酰化位点和人蛋白质乙酰化信号通路的途径，并提供乙酰化位点特异性抗体和重同位素标记肽（AQUA肽），用于选择性检测和定量这些乙酰化位点/蛋白质 ，以及使用试剂用于此目的的方法。 确定的乙酰化位点之间的位点是发生在以下蛋白质类型中的位点：甲基转移酶，转录因子，转录共激活因子，翻译起始复合蛋白​​，氧化还原酶，蛋白激酶，RNA结合蛋白，分泌蛋白，转移酶，转运蛋白，泛素缀合系统蛋白，马达 蛋白质，磷酸酶，蛋白酶，磷脂酶，受体蛋白和囊泡蛋白。

公开(公告)号：US20090081659A1

公开(公告)日：2009-03-26

申请号：US12075065

申请日：2008-03-07

申请人： Peter Hornbeck ,  Ailan Guo ,  Albrecht Moritz ,  John Rush ,  Charles Farnsworth ,  Matthew Stokes ,  Anthony Passemato

发明人： Peter Hornbeck ,  Ailan Guo ,  Albrecht Moritz ,  John Rush ,  Charles Farnsworth ,  Matthew Stokes ,  Anthony Passemato

IPC分类号： G01N33/574 ,  C07K16/18 ,  C12Q1/68

CPC分类号： C07K16/18 ,  C07K16/22 ,  C07K16/30 ,  C07K16/40 ,  C07K2317/34

摘要： The invention discloses 364 novel phosphorylation sites identified in carcinoma, peptides (including AQUA peptides) comprising a phosphorylation site of the invention, antibodies specifically bind to a novel phosphorylation site of the invention, and diagnostic and therapeutic uses of the above.

摘要翻译： 本发明公开了在癌中鉴定的364个新的磷酸化位点，包含本发明的磷酸化位点的肽（包括AQUA肽），特异性结合本发明的新的磷酸化位点的抗体，以及上述的诊断和治疗用途。

公开(公告)号：US06495737B1

公开(公告)日：2002-12-17

申请号：US08909125

申请日：1997-08-11

申请人： Daniel F. Klessig ,  Ailan Guo

发明人： Daniel F. Klessig ,  Ailan Guo

IPC分类号： C12N1582

CPC分类号： C12N15/8279 ,  C12N9/14

摘要： The present invention provides methods and materials that enhance a plant's resistance to certain pathogens. A novel pathway is described and has been designated with the acronym, SI-SAR pathway, for salicylic acid-independent systemic acquired resistance. DNA constructs and methodologies are provided that facilitate the identification of compounds that activate this pathway. Methods are provided to enable the identification of novel genes and signaling components that are expressed when the SI-SAR pathway is activated. Transgenic plants with altered expression of these novel genes or signaling components of the pathway are expected to have enhanced resistance to plant pathogens. Also provided is a novel, pathogen-induced epoxide hydrolase that is inducible in the absence of SA.

摘要翻译： 本发明提供增强植物对某些病原体的抗性的方法和材料。 描述了一条新途径，并且已经用缩写SI-SAR途径指定了水杨酸独立系统获得性抗性。 提供DNA构建体和方法，其有助于鉴定激活该途径的化合物。 提供了方法来确定当SI-SAR途径被激活时表达的新基因和信号传导成分。 预期这些新基因或该途径的信号成分表达改变的转基因植物对植物病原体具有增强的抗性。 还提供了一种新型的病原体诱导的环氧化物水解酶，其在不存在SA时可诱导。