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公开(公告)号：US12116582B2
公开(公告)日：2024-10-15
申请号：US18045447
申请日：2022-10-10
发明人： Stanislaw Flasinski
IPC分类号： C12N15/82
CPC分类号： C12N15/8216 , C12N15/8218 , C12N15/8225 , C12N15/8227 , C12N15/8274 , C12N15/8279
摘要： The invention provides recombinant DNA molecules and constructs, as well as their nucleotide sequences, useful for modulating gene expression in plants. The invention also provides transgenic plants, plant cells, plant parts, and seeds comprising the recombinant DNA molecules operably linked to heterologous transcribable DNA molecules, as are methods of their use.
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公开(公告)号：US20240301437A1
公开(公告)日：2024-09-12
申请号：US18679743
申请日：2024-05-31
发明人： LING LI , EVE SYRKIN WURTELE
IPC分类号： C12N15/82 , C07K14/415
CPC分类号： C12N15/8251 , C07K14/415 , C12N15/8205 , C12N15/8216 , C12N15/8241 , C12N15/8247 , C12N15/8279
摘要： Method of increasing protein content in a eukaryotic cell comprising an NF-YC4 gene comprising modifying the transcriptional repressor binding site; method of producing a plant with increased protein content comprising crossing and selecting for increased protein content; method of increasing resistance to a pathogen or a pest in a plant cell or plant comprising an NF-YC4 gene comprising modifying the transcriptional repressor binding site, alone or in further combination with expressing QQS in the plant cell or plant; method for producing a plant with increased resistance to a pathogen or a pest comprising crossing and selecting for increased resistance to the pathogen or the pest; a cell, collection of cells, tissue, organ, or organism, such as a plant, in which the NF-YC4 gene comprises a promoter comprising a transcriptional repressor binding site that has been modified so that the transcriptional repressor cannot prevent transcription of the NF-YC4; plants and hybrids thereof; and seeds.
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公开(公告)号：US12084670B2
公开(公告)日：2024-09-10
申请号：US18093784
申请日：2023-01-05
发明人： Takashi Hashimoto , Akira Kato
CPC分类号： C12N15/8218 , A24B13/00 , A24B15/10 , A61K31/455 , C12N9/0004 , C12N9/0012 , C12N9/0022 , C12N15/8216 , C12N15/8243 , C12N15/8279 , C12N15/8286 , C12Y104/03006 , Y02A40/146
摘要： MPO1 and MPO2 can be regulated for either decreasing or increasing alkaloid levels in plants, in particular in Nicotiana plants. In particular, suppressing or overexpressing one or more of MPO1 and MPO2 may be used to decrease or increase nicotine and nicotinic alkaloid levels in tobacco plants. Suppression or overexpression of one or more of MPO1 and MPO2 may be used in combination with modification of expression of other genes encoding enzymes on the nicotinic alkaloid biosynthetic pathway such as A622, NBB1, PMT, and QPT.
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公开(公告)号：US20240287532A1
公开(公告)日：2024-08-29
申请号：US18008935
申请日：2021-06-11
发明人： Yanshun LIU , Brody John DEYOUNG , Shirong ZHANG , Laura SCHOUTEN , Peifeng REN , Joerg BAUER , Evan M. ZHAO , James J. COLLINS , Xiao TAN , Fei RAN , Angelo S. MAO , Helena DE PUIG GUIXE , Emma J. CHORY
CPC分类号： C12N15/8203 , C12N5/04 , C12N15/8279 , C12N2770/32043
摘要： The present disclosure provides genetic constructs comprising a recombinant internal ribosome entry site (IRES), which may be used as riboswitches to modulate translation of an operably-mRNA sequence encoding a protein of interest in a plant. In other aspects, the disclosure provides recombinant plant cells, methods, kits and systems that utilize the same, e.g., to provide a platform for modulating the expression of essentially any protein of interest in a plant cell.
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公开(公告)号：US12048280B2
公开(公告)日：2024-07-30
申请号：US17390526
申请日：2021-07-30
发明人： Ellen L. Evans , Kenneth Kmiecik , Chad Kramer , Arie Oppelaar
CPC分类号： A01H5/10 , A01H6/545 , C12N15/8274 , C12N15/8279
摘要： Green bean plants exhibiting resistance to Sclerotinia sclerotiorum are provided, together with methods of producing, identifying, or selecting plants or germplasm with a Sclerotinia sclerotiorum resistance phenotype and lacking an undesirable seed color trait. Such plants include green bean plants comprising introgressed genomic regions conferring disease resistance. Compositions, including novel polymorphic markers for detecting plants comprising introgressed disease resistance alleles, are further provided.
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公开(公告)号：US12037594B2
公开(公告)日：2024-07-16
申请号：US18057439
申请日：2022-11-21
发明人： Ling Li , Eve Syrkin Wurtele
IPC分类号： C12N15/82 , C07K14/415
CPC分类号： C12N15/8251 , C07K14/415 , C12N15/8205 , C12N15/8216 , C12N15/8241 , C12N15/8247 , C12N15/8279
摘要： Method of increasing protein content in a eukaryotic cell comprising an NF-YC4 gene comprising modifying the transcriptional repressor binding site; method of producing a plant with increased protein content comprising crossing and selecting for increased protein content; method of increasing resistance to a pathogen or a pest in a plant cell or plant comprising an NF-YC4 gene comprising modifying the transcriptional repressor binding site, alone or in further combination with expressing QQS in the plant cell or plant; method for producing a plant with increased resistance to a pathogen or a pest comprising crossing and selecting for increased resistance to the pathogen or the pest; a cell, collection of cells, tissue, organ, or organism, such as a plant, in which the NF-YC4 gene comprises a promoter comprising a transcriptional repressor binding site that has been modified so that the transcriptional repressor cannot prevent transcription of the NF-YC4; plants and hybrids thereof; and seeds.
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7.发明公开公开(公告)号：US20240218356A1
公开(公告)日：2024-07-04
申请号：US18558574
申请日：2022-05-11
CPC分类号： C12N15/1093 , C12N15/8279
摘要： Methods are provided for preparing a library of candidate plant disease resistance (R) genes against a plant pathogen of interest. The methods involve selecting from each of one or more plants of interest a subpopulation of highly expressed nucleotide-binding leucine rich repeat genes (NLRs) from among the population of NLRs that are constitutively expressed in an organ or other part of the one or more plants to produce a library of candidate R genes. Further provided are related methods for identifying R genes against a plant pathogen of interest using a library of candidate R genes and compositions comprising the identified R genes.
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8.发明公开公开(公告)号：US20240132418A1
公开(公告)日：2024-04-25
申请号：US18476259
申请日：2023-09-26
申请人： Spogen Biotech Inc.
发明人： Brian M. Thompson , Jorg Augustin
IPC分类号： C05F11/08 , A01N63/50 , C05B15/00 , C05C9/00 , C05F17/20 , C05G3/60 , C07K14/415 , C12N1/20 , C12N9/78 , C12N15/82
CPC分类号： C05F11/08 , A01N63/50 , C05B15/00 , C05C9/00 , C05F17/20 , C05G3/60 , C07K14/415 , C12N1/20 , C12N9/78 , C12N15/8237 , C12N15/8249 , C12N15/8279 , C12Y302/01 , C12Y305/99007 , C12Y402/0202 , Y02A40/22 , Y02P60/21
摘要： Methods for stimulating plant growth and/or promoting plant health using free enzymes or recombinant microorganisms that overexpress enzymes are provided. Plant seeds coated with free enzymes or recombinant microorganisms that overexpress enzymes are also provided. Compositions comprising a fertilizer and an enzyme or a recombinant microorganism that overexpresses an enzyme are provided. Modified enzymes having ACC deaminase activity, recombinant microorganisms expressing the modified enzymes, plant seeds treated with the modified enzymes or recombinant microorganisms, and methods for stimulating plant growth and/or promoting plant health using the modified enzymes or recombinant microorganisms are also provided.
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9.发明公开公开(公告)号：US20240132417A1
公开(公告)日：2024-04-25
申请号：US18476256
申请日：2023-09-26
申请人： Spogen Biotech Inc.
发明人： Brian M. Thompson , Jorg Augustin
IPC分类号： C05F11/08 , A01N63/50 , C05B15/00 , C05C9/00 , C05F17/20 , C05G3/60 , C07K14/415 , C12N1/20 , C12N9/78 , C12N15/82
CPC分类号： C05F11/08 , A01N63/50 , C05B15/00 , C05C9/00 , C05F17/20 , C05G3/60 , C07K14/415 , C12N1/20 , C12N9/78 , C12N15/8237 , C12N15/8249 , C12N15/8279 , C12Y302/01 , C12Y305/99007 , C12Y402/0202 , Y02A40/22 , Y02P60/21
摘要： Methods for stimulating plant growth and/or promoting plant health using free enzymes or recombinant microorganisms that overexpress enzymes are provided. Plant seeds coated with free enzymes or recombinant microorganisms that overexpress enzymes are also provided. Compositions comprising a fertilizer and an enzyme or a recombinant microorganism that overexpresses an enzyme are provided. Modified enzymes having ACC deaminase activity, recombinant microorganisms expressing the modified enzymes, plant seeds treated with the modified enzymes or recombinant microorganisms, and methods for stimulating plant growth and/or promoting plant health using the modified enzymes or recombinant microorganisms are also provided.
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公开(公告)号：US20240124884A1
公开(公告)日：2024-04-18
申请号：US18538893
申请日：2023-12-13
IPC分类号： C12N15/82
CPC分类号： C12N15/8213 , C12N15/8241 , C12N15/8243 , C12N15/8245 , C12N15/8247 , C12N15/8251 , C12N15/8257 , C12N15/8261 , C12N15/827 , C12N15/8271 , C12N15/8274 , C12N15/8279 , C12N15/8286 , C12N15/8289 , C12N15/829
摘要： The invention provides novel uses of sequence-specific or sequence-directed endonucleases for molecular plant breeding. The invention also provides novel plant transformation vectors and expression cassettes, which include novel combinations of an endonuclease with plant expression and transformation elements. Plants and derivatives thereof produced by such methods are also provided.
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