公开(公告)号：US06833246B2

公开(公告)日：2004-12-21

申请号：US10113221

申请日：2002-03-29

Applicant: Shankar Balasubramanian

Inventor： Shankar Balasubramanian

IPC: C12G168

CPC classification number: C12Q1/6869 ,  C12Q1/6823 ,  C12Q2521/319

Abstract: The invention relates to the sequencing of a target polynucleotide sequence, immobilized on a solid support, using the polymerase reaction to extend a suitable primer and characterizing the sequential addition of labelled bases. The present invention further relates to the presence of a polymerase enzyme that retains a 3′ to 5′ exonuclease function, which is induced to remove an incorporated labelled base after detection of incorporation. A corresponding non-labelled base may then be incorporated into the complementary strand to allow further sequence determinations to be made. Repeating the procedure allows the sequence of the complement to be identified, and thereby the target sequence.

Abstract translation: 本发明涉及使用聚合酶反应扩增合适的引物并表征序列添加标记碱基的固定在固体支持物上的靶多核苷酸序列的测序。 本发明还涉及保留3'至5'外切核酸酶功能的聚合酶的存在，其在检测结合后被诱导去除掺入的标记碱基。 然后可以将相应的未标记的碱基并入互补链中以允许进行进一步的序列测定。 重复该过程允许识别补体序列，从而确定靶序列。