公开(公告)号：US4204041A

公开(公告)日：1980-05-20

申请号：US838544

申请日：1977-10-03

申请人： James E. Bailey ,  Yong K. Cho

发明人： James E. Bailey ,  Yong K. Cho

IPC分类号： C12N11/06 ,  C12N11/14 ,  C07G7/02

CPC分类号： C12N11/06 ,  C12N11/14

摘要： Enzymes are immobilized on activated carbon supports at high load levels and with high stability by a procedure whereby a carbon support is first activated with a water-soluble carbodiimide derivative which forms a highly reactive intermediate with carboxyl and other active organic radicals on the surface of the carbon support; thereafter the complex of the carbon and carbodiimide is treated with an enzyme solution whereby the enzyme displaces the carbodiimide and forms a carbon-enzyme complex wherein the enzyme is immobilized and yet the carbon retains its surface activity. Preferred immobilizing agents are quaternary ammonium forms of aminocarbodiimides or hydrochloric acid salts of water-soluble carbodiimides. The immobilization of the enzymes on activated carbon provides materials which are easily handled and which are stabilized against denaturation by hydrogen peroxide.

摘要翻译： 酶以高负载水平固定在活性炭载体上，并具有高稳定性，其中碳载体首先用水溶性碳二亚胺衍生物活化，该衍生物在羧基和其它活性有机基团的表面上形成高反应性中间体 碳支持; 此后用酶溶液处理碳和碳二亚胺的络合物，由此酶置换碳二亚胺并形成酶固定化的碳 - 酶复合物，而碳保留其表面活性。 优选的固定剂是氨基碳二亚胺的季铵形式或水溶性碳二亚胺的盐酸盐。 将酶固定在活性炭上提供容易处理的物质，并且其被稳定以防止过氧化氢变性。