公开(公告)号：US20140004553A1

公开(公告)日：2014-01-02

申请号：US13922432

申请日：2013-06-20

Applicant: President and Fellows of Harvard College

Inventor： Kevin Kit Parker ,  Adam W. Feinberg ,  George M. Whitesides ,  Sergey S. Shevkoplyas ,  Alexander Feigel

IPC: G01N33/50

CPC classification number: G01N33/5061 ,  C12N5/0062 ,  C12N5/0657 ,  C12N2533/30 ,  C12N2533/52 ,  C12N2539/10 ,  G01N2500/10

Abstract: A free-standing thin film is fabricated from a structure comprising a base layer coated with a sacrificial polymer layer, which is in turn coated with a flexible polymer layer. Cells are then seeded onto the flexible polymer layer and cultured to form a tissue. The flexible polymer layer is then released from the base layer to produce a free-standing thin film comprising the tissue on the flexible polymer layer. In one embodiment, the cells are myocytes, which can be actuated to propel or displace the free-standing film. In another embodiment, the free-standing film is used to treat injured human tissue.

Abstract translation: 独立的薄膜由包括涂覆有牺牲聚合物层的基层的结构制成，牺牲聚合物层又被柔性聚合物层涂覆。 然后将细胞接种到柔性聚合物层上并进行培养以形成组织。 然后将柔性聚合物层从基底层释放出来，以产生包含柔性聚合物层上的组织的自立薄膜。 在一个实施方案中，细胞是肌细胞，其可被致动以推动或置换自立膜。 在另一个实施方案中，自立膜用于治疗受损的人体组织。