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公开(公告)号:US4252902A
公开(公告)日:1981-02-24
申请号:US58292
申请日:1979-07-17
申请人: Setsuro Fujii , Kiyoaki Noda , Akio Yokoshima
发明人: Setsuro Fujii , Kiyoaki Noda , Akio Yokoshima
CPC分类号: C12N9/6445 , Y10S435/815
摘要: A process for purification of crude kallikrein utilizing a specific affinity of kallikrein to p-aminobenzamidine, which comprises bringing a solution, containing kallikrein and contaminated by other undesirable enzymes and proteins, into contact with a water-insoluble carrier combined with p-aminobenzamidine, exclusively eluting out the other enzymes and proteins from the carrier by the use of a buffer solution of lower NaCl concentration and then exclusively eluting out kallikrein by the use of a buffer solution of higher NaCl concentration.
摘要翻译: 一种利用激肽释放酶特异性亲和力纯化对氨基苯甲脒的粗提拉曲林的方法,其包括将含有激肽释放酶并被其他不需要的酶和蛋白质污染的溶液与不对称氨基脒组合的水不溶性载体接触, 通过使用较低NaCl浓度的缓冲溶液从载体中洗脱出其它酶和蛋白质,然后通过使用较高NaCl浓度的缓冲溶液专门洗脱激肽释放酶。