公开(公告)号：US20140377819A1

公开(公告)日：2014-12-25

申请号：US14358911

申请日：2011-11-17

申请人： Jian Yu

发明人： Jian Yu

IPC分类号： C12P7/62

CPC分类号： C12P7/625

摘要： A process for producing hydroxyalkanoate copolymers, which comprises: (i) pre-treating a sucrose-containing feedstock in an acidic solution; (ii) feeding the pre-treated feedstock into a bioreactor containing polyhydroxyalkanoate producing microbial cells; (iii) cultivating the polyhydroxyalkanoate producing microbial cells to form a cell mass containing the hydroxyalkanoate copolymers; (iv) recoverying the hydroxyalkanoate copolymers from the cell mass. The pre-treating step has the main function of hydrolyzing sucrose into glucose and fructose, which in turn are converted into 4-ketovaleric acid to give a mixture of mono-saccharides and organic precursors for microbial synthesis of hydroxyalkanoate copolymers, and particularly of PHBVV ter-polymers. Complex and expensive purification processes of the substrates obtained from the pre-treating step are not needed. The solutions can be directly used as the feeding solutions for microbial PHA biosynthesis.

摘要翻译： 一种生产羟基链烷酸酯共聚物的方法，其包括：（i）在酸性溶液中预处理含蔗糖的原料; （ii）将预处理的原料进料到含有聚羟基链烷酸酯生产微生物细胞的生物反应器中; （iii）培养产生聚羟基链烷酸酯的微生物细胞以形成含有羟基链烷酸酯共聚物的细胞团; （iv）从细胞团中回收羟基链烷酸酯共聚物。 预处理步骤具有将蔗糖水解成葡萄糖和果糖的主要功能，其又转化为4-酮戊酸，得到单糖和有机前体的混合物，用于微生物合成羟基链烷酸酯共聚物，特别是PHBVV ter 聚合物。 不需要从预处理步骤获得的底物的复杂且昂贵的纯化方法。 该溶液可直接用作微生物PHA生物合成的饲料溶液。

公开(公告)号：US20120252081A1

公开(公告)日：2012-10-04

申请号：US13502323

申请日：2009-10-16

申请人： Jian Yu

发明人： Jian Yu

IPC分类号： C12P7/62

CPC分类号： C12P7/625

摘要： The present invention relates to a process for producing biodegradable polymeric materials including polyhydroxyalkanoates (PHAs) by using the cell debris left from PHA recovery and purification. The process comprises: (a) cultivating PHA-producing microbial cells in a medium solution containing an organic carbon source to form PHAs that are accumulated in the cells as inclusion bodies; (b) harvesting the cells from the spent medium and solubilizing the non-PHA cell mass to obtain a PHA solid and a cell debris solution; (c) separating the PHA solid from the cell debris solution; (d) feeding the cell debris solution to the cultivation step (a). By reusing the cell debris generated from PHA recovery, the invention avoids disposal of a large amount of aqueous waste. In addition, a remarkable increase of cell growth and PHA synthesis is achieved, because the cell debris can be readily assimilated by the microbial cells as the nutrients.

摘要翻译： 本发明涉及通过使用从PHA回收和纯化留下的细胞碎片来生产包括聚羟基链烷酸酯（PHA）的生物可降解聚合物材料的方法。 该方法包括：（a）在含有机碳源的培养基溶液中培养产生PHA的微生物细胞，形成作为包涵体积聚在细胞中的PHA; （b）从废培养基中收获细胞并溶解非PHA细胞块以获得PHA固体和细胞碎片溶液; （c）将PHA固体与细胞碎片溶液分离; （d）将细胞碎片溶液送入培养步骤（a）。 通过重新利用由PHA回收产生的细胞碎片，本发明避免了大量的含水废物的处置。 此外，实现了细胞生长和PHA合成的显着增加，因为细胞碎片可以容易地被微生物细胞作为营养物质吸收。