公开(公告)号：US12024545B2

公开(公告)日：2024-07-02

申请号：US17336183

申请日：2021-06-01

申请人： HCW Biologics, Inc.

发明人： Hing C. Wong ,  Xiaoyun Zhu ,  Bai Liu ,  Pallavi Chaturvedi ,  Varghese George

IPC分类号： A61K38/17 ,  A61K39/395 ,  A61K45/06 ,  C07K14/47 ,  A61K38/00

CPC分类号： C07K14/4703 ,  A61K38/179 ,  A61K39/3955 ,  A61K45/06 ,  A61K38/00 ,  C07K2317/24 ,  C07K2317/73

摘要： Provided herein are methods of killing or reducing the number of naturally-occurring and/or treatment-induced senescent cells and diseased cells in a subject in need thereof, decreasing the accumulation of naturally-occurring and/or treatment-induced senescent cells and diseased cells in a subject in need thereof, that include administering to the subject a therapeutically effective amount of one or more common gamma-chain family cytokine receptor activating agent(s) and/or one or more agent(s) that result(s) in a decrease in the activation of a TGF-β receptor.

公开(公告)号：US20230372444A1

公开(公告)日：2023-11-23

申请号：US18300354

申请日：2023-04-13

申请人： HCW Biologics, Inc.

发明人： Hing C. Wong ,  Varghese George ,  Niraj Shrestha ,  Pallavi Chaturvedi

IPC分类号： A61K38/17 ,  A61K38/20 ,  A61P25/16

CPC分类号： A61K38/1793 ,  A61K38/20 ,  A61P25/16

摘要： Provided herein are multi-chain chimeric polypeptides and use thereof in reducing neuroinflammation in a subject.

公开(公告)号：US20210070826A1

公开(公告)日：2021-03-11

申请号：US16952861

申请日：2020-11-19

申请人： HCW Biologics, Inc.

发明人： Hing Wong

IPC分类号： C07K14/54 ,  C07K14/71 ,  C07K14/715 ,  C07K14/745 ,  C07K16/28

摘要： Provided herein are multi-chain chimeric polypeptides that include:
(a) a first chimeric polypeptide including a first target-binding domain, a soluble tissue factor domain, and a first domain of a pair of affinity domains; and (b) a second chimeric polypeptide including a second domain of a pair of affinity domains and a second target-binding domain, where the first chimeric polypeptide and the second chimeric polypeptide associate through the binding of the first domain and the second domain of the pair of affinity domains. Also provided here are methods of using these multi-chain chimeric polypeptides and nucleic acids encoding these multi-chain chimeric polypeptides.

公开(公告)号：US20240132561A1

公开(公告)日：2024-04-25

申请号：US18497163

申请日：2023-10-30

申请人： HCW Biologics, Inc.

发明人： Hing Wong

IPC分类号： C07K14/54 ,  C07K14/71 ,  C07K14/715 ,  C07K14/745 ,  C07K16/28

CPC分类号： C07K14/5434 ,  C07K14/54 ,  C07K14/5418 ,  C07K14/71 ,  C07K14/7155 ,  C07K14/745 ,  C07K16/283 ,  C07K2317/622 ,  C07K2319/02 ,  C07K2319/03 ,  C07K2319/30

摘要： Provided herein are multi-chain chimeric polypeptides that include:



(a) a first chimeric polypeptide including a first target-binding domain, a soluble tissue factor domain, and a first domain of a pair of affinity domains; and (b) a second chimeric polypeptide including a second domain of a pair of affinity domains and a second target-binding domain, where the first chimeric polypeptide and the second chimeric polypeptide associate through the binding of the first domain and the second domain of the pair of affinity domains. Also provided here are methods of using these multi-chain chimeric polypeptides and nucleic acids encoding these multi-chain chimeric polypeptides.

公开(公告)号：US20230372399A1

公开(公告)日：2023-11-23

申请号：US18308894

申请日：2023-04-28

申请人： HCW Biologics, Inc.

发明人： Hing Wong

IPC分类号： A61K35/17 ,  C07K14/705 ,  C07K14/715 ,  C07K16/22 ,  C07K16/24 ,  C07K16/28 ,  C07K16/46

CPC分类号： A61K35/17 ,  C07K14/70503 ,  C07K14/7155 ,  C07K16/22 ,  C07K16/244 ,  C07K16/2863 ,  C07K16/468 ,  C07K2317/24 ,  C07K2317/52 ,  C07K2317/569 ,  C07K2317/622

摘要： Provided herein are methods of treating an aging-related disease or condition in a subject in need thereof, killing or reducing the number of senescent cells in a subject in need thereof, improving the texture and/or appearance of skin and/or hair in a subject in need thereof, and assisting in the treatment of obesity in a subject in need thereof, that include administering to the subject a therapeutically effective amount of one or more natural killer (NK) cell activating agent(s) and/or a therapeutically effective number of activated NK cells.

公开(公告)号：US20230272027A1

公开(公告)日：2023-08-31

申请号：US18047754

申请日：2022-10-19

申请人： HCW Biologics, Inc.

发明人： Hing Wong

IPC分类号： C07K14/54 ,  C07K14/71 ,  C07K14/715 ,  C07K14/745 ,  C07K16/28

CPC分类号： C07K14/5434 ,  C07K14/54 ,  C07K14/5418 ,  C07K14/71 ,  C07K14/7155 ,  C07K14/745 ,  C07K16/283 ,  C07K2317/622 ,  C07K2319/02 ,  C07K2319/03 ,  C07K2319/30

摘要： Provided herein are multi-chain chimeric polypeptides that include:



(a) a first chimeric polypeptide including a first target-binding domain, a soluble tissue factor domain, and a first domain of a pair of affinity domains; and (b) a second chimeric polypeptide including a second domain of a pair of affinity domains and a second target-binding domain, where the first chimeric polypeptide and the second chimeric polypeptide associate through the binding of the first domain and the second domain of the pair of affinity domains. Also provided here are methods of using these multi-chain chimeric polypeptides and nucleic acids encoding these multi-chain chimeric polypeptides.

公开(公告)号：US11730762B2

公开(公告)日：2023-08-22

申请号：US16557822

申请日：2019-08-30

申请人： HCW Biologies, Inc.

发明人： Hing Wong

IPC分类号： C07K14/715 ,  C07K14/54 ,  A61K35/17 ,  C07K14/705 ,  C07K16/22 ,  C07K16/24 ,  C07K16/28 ,  C07K16/46

CPC分类号： A61K35/17 ,  C07K14/70503 ,  C07K14/7155 ,  C07K16/22 ,  C07K16/244 ,  C07K16/2863 ,  C07K16/468 ,  C07K2317/24 ,  C07K2317/52 ,  C07K2317/569 ,  C07K2317/622

摘要： The present disclosure relates to the field of biotechnology, and more specifically, to single-chain and multi-chain chimeric polypeptides having a linker domain positioned between two target-binding domains that are useful for a variety of applications including, without limitation, stimulating an immune cell, inducing or increasing proliferation of an immune cell, inducing differentiation of an immune cell, or treating a subject in need thereof (e.g., a subject having cancer or an aging-related disease or condition).

公开(公告)号：US12018071B2

公开(公告)日：2024-06-25

申请号：US17871211

申请日：2022-07-22

申请人： HCW Biologics, Inc.

发明人： Hing Wong

IPC分类号： C07K16/18 ,  A61P3/10 ,  C07K16/28 ,  C12N5/0783 ,  A61K39/00

CPC分类号： C07K16/18 ,  A61P3/10 ,  C07K16/2809 ,  C07K16/2818 ,  C07K16/2866 ,  C12N5/0637 ,  A61K39/00 ,  C07K2317/24 ,  C07K2317/56 ,  C07K2317/622 ,  C07K2319/00 ,  C12N2501/998

摘要： Provided herein are single-chain chimeric polypeptides that include: (i) a first target-binding domain; (ii) a soluble tissue factor domain; and (iii) a second target-binding domain. Also provided here are methods of using these single-chain chimeric polypeptides and nucleic acids encoding these single-chain chimeric polypeptides.

公开(公告)号：US11884712B2

公开(公告)日：2024-01-30

申请号：US16952861

申请日：2020-11-19

申请人： HCW Biologics, Inc.

发明人： Hing Wong

IPC分类号： C07K14/54 ,  C07K14/71 ,  C07K14/715 ,  C07K14/745 ,  C07K16/28

CPC分类号： C07K14/5434 ,  C07K14/54 ,  C07K14/5418 ,  C07K14/71 ,  C07K14/7155 ,  C07K14/745 ,  C07K16/283 ,  C07K2317/622 ,  C07K2319/02 ,  C07K2319/03 ,  C07K2319/30

摘要： Provided herein are multi-chain chimeric polypeptides that include:



(a) a first chimeric polypeptide including a first target-binding domain, a soluble tissue factor domain, and a first domain of a pair of affinity domains; and (b) a second chimeric polypeptide including a second domain of a pair of affinity domains and a second target-binding domain, where the first chimeric polypeptide and the second chimeric polypeptide associate through the binding of the first domain and the second domain of the pair of affinity domains. Also provided here are methods of using these multi-chain chimeric polypeptides and nucleic acids encoding these multi-chain chimeric polypeptides.

公开(公告)号：US20230381238A1

公开(公告)日：2023-11-30

申请号：US18354866

申请日：2023-07-19

申请人： HCW Biologics, Inc.

发明人： Hing C. Wong

IPC分类号： A61K35/17 ,  C07K14/54 ,  C07K16/42

CPC分类号： A61K35/17 ,  C07K14/5418 ,  C07K14/5434 ,  C07K14/5443 ,  C07K16/4283 ,  C07K2319/74

摘要： The present disclosure relates to methods that include the use of a first multi-chain chimeric polypeptide and a second multi-chain chimeric polypeptide for stimulating the NK cells, inducing or increasing proliferation of the NK cells, inducing differentiation of the NK cells, and treating a subject in need thereof using activated NK cells.