公开(公告)号：US20230111586A1

公开(公告)日：2023-04-13

申请号：US17964071

申请日：2022-10-12

Applicant: BIO-RAD LABORATORIES, INC. ,  ALIGHT SCIENCES INC.

Inventor： WILLIAM BRADLEY STRONG ,  ALEXANDER EDMUND JOHNSON-BUCK

IPC: B01L3/00 ,  G01N21/64

Abstract: This disclosure pertains to a consumable product (microplate) suitable for use in assays utilizing single-molecule recognition through equilibrium Poisson sampling (SiMREPS) and in other assays employing total internal reflection fluorescence (TIRF) or HiLo microscopy. The disclosed microplate is also suitable for use in other high throughput assay systems, such as single-molecule FRET, ligand-receptor binding studies, membrane biology assays, cell-based TIRF and near-TIRF assays. The disclosure further pertains to the use of the microfluidic microplate for the detection of analytes, including nucleic acids, polypeptides, carbohydrates, lipids, post-translational modifications, amino acids, metabolites, and small molecules.