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公开(公告)号:US07363168B2
公开(公告)日:2008-04-22
申请号:US10262474
申请日:2002-10-01
申请人: Roger Taylor , Alan Ridgeway Orr
发明人: Roger Taylor , Alan Ridgeway Orr
IPC分类号: G06F7/00
CPC分类号: G01N21/6428 , C12Q1/6851 , C12Q2537/165
摘要: The invention relates to baseline subtraction algorithms developed to reduce tube-to-tube and cycle-to-cycle variabilities during real time PCR amplification. Particularly, the invention relates to algorithms for determining the threshold cycle for the first reliable detection of the amplified nucleic acid product. The invention also relates to computer programs comprising the algorithm and methods and/systems implementing the algorithm.
摘要翻译: 本发明涉及在实时PCR扩增期间开发的用于减少管对管和循环到周期变化的基线减法算法。 特别地,本发明涉及用于确定扩增的核酸产物的第一次可靠检测的阈值循环的算法。 本发明还涉及包括实现算法的算法和方法和/系统的计算机程序。