Adaptive baseline algorithm for quantitative PCR
    1.
    发明授权
    Adaptive baseline algorithm for quantitative PCR 有权
    定量PCR的自适应基线算法

    公开(公告)号:US07363168B2

    公开(公告)日:2008-04-22

    申请号:US10262474

    申请日:2002-10-01

    IPC分类号: G06F7/00

    摘要: The invention relates to baseline subtraction algorithms developed to reduce tube-to-tube and cycle-to-cycle variabilities during real time PCR amplification. Particularly, the invention relates to algorithms for determining the threshold cycle for the first reliable detection of the amplified nucleic acid product. The invention also relates to computer programs comprising the algorithm and methods and/systems implementing the algorithm.

    摘要翻译: 本发明涉及在实时PCR扩增期间开发的用于减少管对管和循环到周期变化的基线减法算法。 特别地,本发明涉及用于确定扩增的核酸产物的第一次可靠检测的阈值循环的算法。 本发明还涉及包括实现算法的算法和方法和/系统的计算机程序。