公开(公告)号：US07022287B2

公开(公告)日：2006-04-04

申请号：US10141806

申请日：2002-05-08

申请人： Joseph Schoeniger ,  Albert W. Flounders ,  Robert C. Hughes ,  Antonio J. Ricco ,  Karl Wally ,  Stanley H. Kravitz ,  Richard P. Janek

发明人： Joseph Schoeniger ,  Albert W. Flounders ,  Robert C. Hughes ,  Antonio J. Ricco ,  Karl Wally ,  Stanley H. Kravitz ,  Richard P. Janek

IPC分类号： G01N15/06

CPC分类号： G01N33/5438 ,  G01N27/22

摘要： The present invention discloses an electrochemical device for detecting single particles, and methods for using such a device to achieve high sensitivity for detecting particles such as bacteria, viruses, aggregates, immuno-complexes, molecules, or ionic species. The device provides for affinity-based electrochemical detection of particles with single-particle sensitivity. The disclosed device and methods are based on microelectrodes with surface-attached, affinity ligands (e.g., antibodies, combinatorial peptides, glycolipids) that bind selectively to some target particle species. The electrodes electrolyze chemical species present in the particle-containing solution, and particle interaction with a sensor element modulates its electrolytic activity. The devices may be used individually, employed as sensors, used in arrays for a single specific type of particle or for a range of particle types, or configured into arrays of sensors having both these attributes.