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公开(公告)号：US08008459B2
公开(公告)日：2011-08-30
申请号：US10466959
申请日：2002-01-25
CPC分类号： C12N15/85 , C12N15/66 , C12N15/70 , C12N15/81 , C12N2800/20 , C12N2800/206
摘要： In the present invention are disclosed concatemers of concatenated expression cassettes and vectors that enable the synthesis of such concatemers. The concatemer comprises in the 5′→3′ direction a cassette of nucleotide sequence of the general formula [rs2-SP-PR-X-TR-SP-rs1]n wherein rs1 and rs2 together denote a functional restriction site, SP individually denotes a spacer of at least two nucleotide bases, PR denotes a promoter, capable of functioning in a cell, X denotes an expressible nucleotide sequence, TR denotes a terminator, and SP individually denotes a spacer of at least two nucleotide bases, and n>/=2, and wherein at least a first cassette is different from a second cassette. The main purpose of these concatemers is the controllable and co-ordinated expression of large numbers of heterologous genes in a single host. Furthermore, the invention relates to a concatemer of cassettes of nucleotide sequences and a method for preparing the concatemers. In a further aspect, the invention relates to transgenic host cells comprising at least one concatemer according to the invention, as well as to a method for preparing the transgenic host cells. Finally, the invention relates to a vector comprising a cassette of nucleotides, a method for preparing said vector, a nucleotide library comprising at least two primary vectors each comprising a cassette of nucleotides, a method for preparing the library.
摘要翻译： 在本发明中公开了连接的表达盒和能够合成这种并发物的载体的并列物质。 连接体在5'→3'方向上包含通式[rs2-SP-PR-X-TR-SP-rs1] n的核苷酸序列的盒，其中rs1和rs2一起表示功能性限制性位点，SP分别表示 至少两个核苷酸碱基的间隔区，PR表示能够在细胞中起作用的启动子，X表示可表达的核苷酸序列，TR表示终止子，SP表示至少两个核苷酸碱基的间隔区，n> = 2，并且其中至少第一盒不同于第二盒。 这些结合物的主要目的是在单个宿主中可控和协调的大量异源基因的表达。 此外，本发明涉及核苷酸序列的盒的连接体和制备该并列物质的方法。 在另一方面，本发明涉及包含根据本发明的至少一种连接体的转基因宿主细胞，以及制备转基因宿主细胞的方法。 最后，本发明涉及包含核苷酸盒的载体，制备所述载体的方法，包含至少两个主要载体的核苷酸文库，每个载体包含核苷酸盒，制备文库的方法。
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