公开(公告)号：US06180773B2

公开(公告)日：2001-01-30

申请号：US09180077

申请日：1998-12-30

申请人： Wolf-Georg Forssmann ,  Andreas Pardigol ,  Hans-J{umlaut over (u)}rgen M{umlaut over (a)}gert ,  Peter Schulz-Knappe

发明人： Wolf-Georg Forssmann ,  Andreas Pardigol ,  Hans-J{umlaut over (u)}rgen M{umlaut over (a)}gert ,  Peter Schulz-Knappe

IPC分类号： C12N1519

CPC分类号： C07K14/523 ,  A61K38/00

摘要： The CC type chemokines belong to a family of polypeptides which have proven to be mediators of immune reactions, and they have recently attracted attention due to their antiviral activity with respect to HIV. The cloning and molecular characterization of a human tandem gene is disclosed which contains the closely linked coding regions for two new CC type chemokines the sequences of which are highly homologous with that of MIP-1&agr;. The transcription of the tandem gene leads to a bicistronic mature transcript which contains the non-overlapping open reading frames for the recently described factor HCC-1 and an as yet unknown CC type chemokine, designated as CC-2. Moreover, alternative splicing of the primary transcript yields at least one additional CC type chemokine, cytokine CC-3. Two functional promoter regions were identified within the tandem gene. The disclosed data provide some basic knowledge about the structure and expression of the new human CC-2/HCC-1 tandem gene and describe a mechanism according to which the coexpression of closely linked genes could be regulated in higher eucaryotes.

摘要翻译： CC型趋化因子属于被证明是免疫反应介质的多肽家族，并且由于它们对于HIV的抗病毒活性，它们最近引起关注。 公开了人串联基因的克隆和分子表征，其包含两个新的CC型趋化因子的紧密连锁的编码区，其序列与MIP-1α的序列高度同源。 串联基因的转录导致二顺反子成熟转录物，其包含最近描述的因子HCC-1的非重叠开放阅读框和称为CC-2的尚未知的CC型趋化因子。 此外，主要转录物的选择性剪接产生至少一种另外的CC型趋化因子，细胞因子CC-3。 在串联基因内鉴定了两个功能启动子区域。 所公开的数据提供了关于新型人CC-2 / HCC-1串联基因的结构和表达的一些基本知识，并描述了一种机制，根据该机制可以在较高的真核生物中调节紧密连锁的基因的共表达。