公开(公告)号：US07169554B2

公开(公告)日：2007-01-30

申请号：US10149146

申请日：2000-12-11

申请人： Andrew James Rutter ,  Ian Weeks ,  Zhaoqiang Li ,  Keith Smith

发明人： Andrew James Rutter ,  Ian Weeks ,  Zhaoqiang Li ,  Keith Smith

IPC分类号： C12Q1/68 ,  C07H21/02 ,  C07H21/04

CPC分类号： C12Q1/6818 ,  C12Q2565/101

摘要： Oligonucleotide building processes are monitored by means of an oligonucleotide probe which in one embodiment is labelled at one end with a chemiluminescent label and at the other end with a quencher molecule. The conformation of the oligonucleotide probe changes according to whether the probe hybridises with a substantially complementary nucleic acid sequence. In the non-hybridised state the chemiluminescent label is sufficiently close proximity to the quencher that the chemiluminescent emission is substantially attenuated, but in the hybridised state the separation is such that there is little or no attenuation. Particular probes and emitter/quencher pairs are disclosed.

摘要翻译： 通过寡核苷酸探针监测寡核苷酸构建过程，寡核苷酸探针在一个实施方案中在一端用化学发光标记物标记，另一端用猝灭剂分子标记。 寡核苷酸探针的构象根据探针是否与基本互补的核酸序列杂交而变化。 在非杂交状态下，化学发光标记与猝灭剂充分接近，化学发光发射基本上被衰减，但在杂交状态下，分离使得几乎没有或没有衰减。 公开了特定的探针和发射/猝灭剂对。