公开(公告)号：US20090087858A1

公开(公告)日：2009-04-02

申请号：US12330460

申请日：2008-12-08

申请人： Andrew K. Finn ,  Caifu Chen

发明人： Andrew K. Finn ,  Caifu Chen

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6818 ,  C12Q1/6851 ,  C12Q2565/1025 ,  C12Q2537/143 ,  C12Q2525/161 ,  C12Q2525/301

摘要： The present invention is directed to methods, reagents, kits, and compositions for detecting target polynucleotide sequences, especially small target polynucleotides such as miRNAs, between two samples. A pair of linker probes can be employed in two different reactions to query a particular species of target polynucleotide. A pair of detector probes, a single forward primer specific for the target polynucleotide, and a reverse primer can be employed in an amplification reaction to query the difference in expression level of the target polynucleotide between the two samples. In some embodiments a plurality of small miRNAs are queried with a plurality of linker probes. The plurality of queried miRNAs can then be decoded in a plurality of amplification reactions.

摘要翻译： 本发明涉及用于在两个样品之间检测靶多核苷酸序列，特别是小目标多核苷酸如miRNA的方法，试剂，试剂盒和组合物。 可以在两个不同的反应中使用一对接头探针来查询特定种类的靶多核苷酸。 可以在扩增反应中使用一对检测器探针，针对靶多核苷酸特异性的单一正向引物和反向引物来查询两个样品之间的靶多核苷酸表达水平的差异。 在一些实施方案中，使用多个接头探针查询多个小miRNA。 然后可以在多个扩增反应中解码多个查询的miRNA。

公开(公告)号：US07642055B2

公开(公告)日：2010-01-05

申请号：US11232475

申请日：2005-09-21

申请人： Andrew K. Finn ,  Caifu Chen

发明人： Andrew K. Finn ,  Caifu Chen

IPC分类号： C12P19/34 ,  C07H21/04

CPC分类号： C12Q1/6818 ,  C12Q1/6851 ,  C12Q2565/1025 ,  C12Q2537/143 ,  C12Q2525/161 ,  C12Q2525/301

摘要： The present invention is directed to methods, reagents, kits, and compositions for detecting target polynucleotide sequences, especially small target polynucleotides such as miRNAs, between two samples. A pair of linker probes can be employed in two different reactions to query a particular species of target polynucleotide. A pair of detector probes, a single forward primer specific for the target polynucleotide, and a reverse primer can be employed in an amplification reaction to query the difference in expression level of the target polynucleotide between the two samples. In some embodiments a plurality of small miRNAs are queried with a plurality of linker probes. The plurality of queried miRNAs can then be decoded in a plurality of amplification reactions.

摘要翻译： 本发明涉及用于在两个样品之间检测靶多核苷酸序列，特别是小目标多核苷酸如miRNA的方法，试剂，试剂盒和组合物。 可以在两个不同的反应中使用一对接头探针来查询特定种类的靶多核苷酸。 可以在扩增反应中使用一对检测器探针，针对靶多核苷酸特异性的单一正向引物和反向引物来查询两个样品之间的靶多核苷酸表达水平的差异。 在一些实施方案中，使用多个接头探针查询多个小miRNA。 然后可以在多个扩增反应中解码多个查询的miRNA。

公开(公告)号：US20100203545A1

公开(公告)日：2010-08-12

申请号：US12760524

申请日：2010-04-14

申请人： Andrew K. Finn ,  Caifu Chen

发明人： Andrew K. Finn ,  Caifu Chen

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6818 ,  C12Q1/6851 ,  C12Q2565/1025 ,  C12Q2537/143 ,  C12Q2525/161 ,  C12Q2525/301

摘要： The present invention is directed to methods, reagents, kits, and compositions for detecting target polynucleotide sequences, especially small target polynucleotides such as miRNAs, between two samples. A pair of linker probes can be employed in two different reactions to query a particular species of target polynucleotide. A pair of detector probes, a single forward primer specific for the target polynucleotide, and a reverse primer can be employed in an amplification reaction to query the difference in expression level of the target polynucleotide between the two samples. In some embodiments a plurality of small miRNAs are queried with a plurality of linker probes. The plurality of queried miRNAs can then be decoded in a plurality of amplification reactions.

摘要翻译： 本发明涉及用于在两个样品之间检测靶多核苷酸序列，特别是小目标多核苷酸如miRNA的方法，试剂，试剂盒和组合物。 可以在两个不同的反应中使用一对接头探针来查询特定种类的靶多核苷酸。 可以在扩增反应中使用一对检测器探针，针对靶多核苷酸特异性的单一正向引物和反向引物来查询两个样品之间的靶多核苷酸表达水平的差异。 在一些实施方案中，使用多个接头探针查询多个小miRNA。 然后可以在多个扩增反应中解码多个查询的miRNA。