公开(公告)号：US07015339B2

公开(公告)日：2006-03-21

申请号：US10201191

申请日：2002-07-23

申请人： Anil Bhagwan Khare ,  Brent Howard Hilbert

发明人： Anil Bhagwan Khare ,  Brent Howard Hilbert

IPC分类号： C07D319/14

CPC分类号： A61K36/00 ,  C07D311/24 ,  C07D311/30 ,  A61K2300/00

摘要： The invention provides compositions and methods for isolating phenolic compounds, particularly isoflavones, from aqueous extracts of plant materials that contain such compounds. The process comprising: (a) providing an aqueous plant extract at a first pH less than 10, the aqueous plant extract comprising a plurality of phenolic compounds; (b) extracting the aqueous plant extract with a first organic solvent to yield a first organic extract; (c) extracting the first organic extract with an aqueous phase of pH greater than 10 to yield a phenol rich aqueous phase; (d) adjusting the pH of the phenol rich aqueous phase to a pH of less than 9; and (e) isolating the phenolic compounds from the phenol rich aqueous phase.

公开(公告)号：US07888081B2

公开(公告)日：2011-02-15

申请号：US11752492

申请日：2007-05-23

申请人： Anil Bhagwan Khare ,  Brent H. Hilbert ,  Christopher Solheid ,  Sara C. McFarlan ,  Fernando A. Sanchez-Riera ,  Kent Floy ,  Paula M. Hicks

发明人： Anil Bhagwan Khare ,  Brent H. Hilbert ,  Christopher Solheid ,  Sara C. McFarlan ,  Fernando A. Sanchez-Riera ,  Kent Floy ,  Paula M. Hicks

IPC分类号： C12P13/22 ,  C12N1/21

CPC分类号： C07D209/20 ,  B01D15/34 ,  B01D15/362 ,  B01D15/363 ,  B01D15/3804 ,  C12P17/10

摘要： Methods and systems for increasing the production of equilibrium reactions are described. In some embodiments, a method comprises removing enzymes from a reaction mixture once equilibrium is achieved, selectively re-adding enzymes and reactants for driving the product-forming reaction to product, and optionally recycling stabilized intermediates, by-products and/or co-products back into the reaction mixture. In some embodiments, a method comprises purifying the desired product from a reaction mixture and selectively recycling one or more components of the reaction mixture, such as original reactants, intermediates, co-products or by-products back into the reaction mixture as desired to improve titer. Systems for implementing the methods are also provided. In some embodiments the methods and systems are implemented for increasing the production of monatin and monatin derivatives, produced in multi-step equilibrium pathways.

摘要翻译： 描述了用于增加平衡反应生成的方法和系统。 在一些实施方案中，一种方法包括一旦达到平衡即从反应混合物中除去酶，选择性地重新加入用于将产物形成反应驱动到产物中的酶和反应物，以及任选地再循环稳定的中间体，副产物和/或副产物 回到反应混合物中。 在一些实施方案中，方法包括从反应混合物中纯化所需产物，并根据需要选择性地将反应混合物中的一种或多种组分（例如原始反应物，中间体，副产物或副产物）循环回到反应混合物中，以改善 滴度。 还提供了实现方法的系统。 在一些实施方案中，实施方法和系统用于增加在多步平衡途径中产生的莫纳甜和莫纳甜衍生物的产生。