公开(公告)号：US20120272348A1

公开(公告)日：2012-10-25

申请号：US13384468

申请日：2010-07-16

申请人： Olivier Danos ,  Araksya Izmiryan ,  Alix Bourdel

发明人： Olivier Danos ,  Araksya Izmiryan ,  Alix Bourdel

IPC分类号： C12N9/96 ,  A01K67/00 ,  C12N5/10 ,  C12N15/62 ,  C12N15/867 ,  C12N15/85

CPC分类号： C12N9/22 ,  A01K2217/00 ,  A61K48/00 ,  C07K14/005 ,  C07K2319/00 ,  C07K2319/01 ,  C12N15/86 ,  C12N2740/16043 ,  C12N2740/16322

摘要： The present invention relates to a fusion protein which comprises at least a functional meganuclease and a viral protein and in particular to fusion protein comprising at least a meganuclease, which recognises and cleaves a specific DNA target sequence and a viral peptide selected from the group Vpr and Vpx or a fragment or derivative thereof; wherein said fusion protein is able to associate with Lentivirus vector particles and following transduction into a host cell recognise and cleave said specific DNA target in vivo. The present Patent Application also relates to a viral particle comprising such a fusion protein and to the use of such fusion proteins and viral particles for gene targeting.

摘要翻译： 本发明涉及融合蛋白，其至少包含功能性大范围核酸酶和病毒蛋白，特别是至少包含识别和切割特异性DNA靶序列的大范围核酸酶的融合蛋白和选自Vpr和 Vpx或其片段或衍生物; 其中所述融合蛋白能够与慢病毒载体颗粒结合，并且在转导入宿主细胞后，在体内识别和切割所述特异性DNA靶标。 本专利申请还涉及包含这种融合蛋白的病毒颗粒以及这种融合蛋白和病毒颗粒用于基因靶向的用途。