公开(公告)号：US5888369A

公开(公告)日：1999-03-30

申请号：US828398

申请日：1997-03-28

申请人： Barbara L. Tippins ,  Roumen Bogoev ,  Douglas R. Levy

发明人： Barbara L. Tippins ,  Roumen Bogoev ,  Douglas R. Levy

IPC分类号： G01N27/447 ,  G01N27/22

CPC分类号： G01N27/44708

摘要： An apparatus of conducting electrophoresis experiments including a container for receiving an electrophoresis buffer and a buffer core assembly for holding gel-containing cassettes that are immersed in the buffer for molecular separation of an electrophoresis sample. The buffer core assembly defines a flow path for the electrophoresis buffer which can be circulated by means of a heat exchanger and a pump to effect controlled heat exchange between the buffer and the cassette surfaces thereby maintaining the gel and the electrophoresis process at a desirable temperature uniformly over the surfaces of the gel cassette. The buffer core assembly also has upper well for receiving a second electrically chargeable buffer in contact with the gel so as to impose an electric field on the gel for the electrophoresis in conjunction with the first buffer. The well is isolated from the first buffer to prevent mixing of the buffers and to reduce the risk of an electrical short.

摘要翻译： 一种进行电泳实验的装置，包括用于接收电泳缓冲液的容器和用于保持浸入缓冲液中用于分离分离电泳样品的凝胶含有盒的缓冲芯组件。 缓冲芯组件限定了用于电泳缓冲液的流动路径，其可以通过热交换器和泵循环，以实现缓冲器和盒表面之间的受控热交换，从而将凝胶和电泳过程保持在期望的温度下均匀 在凝胶盒的表面上。 缓冲芯组件还具有用于接收与凝胶接触的第二可充电缓冲液的上孔，以便在与第一缓冲液结合的电泳上在凝胶上施加电场。 该井与第一缓冲液隔离，以防止缓冲液混合并降低电气短路的风险。